Development Time Variability: Adaptation of Régnière’s Method to the Intrinsic Variability of Belgian Lucilia Sericata (Diptera, Calliphoridae) Population

Gosselin, Matthias; Charabidzé, Damien; Frippiat, Christophe; Bourel, Benjamin; Gosset, D.

doi:10.4172/2157-7145.1000109

Cited by 7 publications

(9 citation statements)

References 22 publications

(30 reference statements)

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“…In the control experiments, the survival rate was close to 90%. This value is consistent with previous observations on L. sericata or other Calliphoridae species . Furthermore, the remaining 10% baseline mortality may be due to injuries during the transfer of the first instar from the hatching media to rearing boxes.…”

Section: Discussionsupporting

confidence: 92%

In Vitro Effects of Household Products on Calliphoridae Larvae Development: Implication for Forensic Entomology

Aubernon

Devigne

Hédouin

et al. 2014

Journal of Forensic Sciences

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Several parameters can delay the first arrival of flies on a corpse and the subsequent development of the larvae. This study focuses on the development of Lucilia sericata (Diptera: Calliphoridae) (Meigen, 1826) on household chemical-contaminated substrates. bleach, perfume, hydrochloric acid, caustic soda, insecticide, mosquito repellent, and gasoline in quantities consistent with an amount that could possibly be spilled on a corpse were mixed with beef liver to simulate contaminated fleshes. Larvae were bred at 25 °C on these media until emergence. Four developmental parameters were followed: survival rates, development times, sex ratios, and adult sizes. Hydrochloric acid, insecticide, and gasoline killed all larvae. In low quantities, caustic soda and mosquito repellent increased the development time and decreased the adult size. However, high quantities of these chemicals killed all larvae. Lastly, bleach and perfume did not affect the survival rate and barely impacted the development time or adult size. These results demonstrate common household products spilled on a corpse can strongly affect the development of Calliphoridae larvae. The effects of such products should be considered in forensic entomology cases.

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Section: Discussionsupporting

confidence: 92%

In Vitro Effects of Household Products on Calliphoridae Larvae Development: Implication for Forensic Entomology

Aubernon

Devigne

Hédouin

et al. 2014

Journal of Forensic Sciences

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“…Our result showed a Ts between 8.3 °C and 8.9 °C for ADD i between 223 and 243 d. These thermal constants are in the same range as those obtained with development data by Grassberger (ADD i : 214 d; Ts : 9.14 °C) [ 9 ] and Gosselin et al. [ 17 ] (ADD i : 217.97 d; Ts : 9.55 °C) but are very different from the thermal constant found by Anderson [ 12 ] (ADD i : 352.97 d; Ts : 4.48 °C) and Greenberg [ 10 ] (ADD i : 485 d; Ts : 11.3 °C). The conditions of rearing, photoperiod and examination interval can explain these differences.…”

Section: Discussionsupporting

confidence: 88%

Effect of low temperature in the development cycle ofLucilia sericata(Meigen) (Diptera, Calliphoridae): implications for the minimum postmortem interval estimation

Cervantès

Dourel

Gaudry

et al. 2017

Forensic Sciences Research

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Knowledge of necrophagous insects’ developmental data is necessary for the forensic entomologist to estimate a reliable minimum postmortem interval (PMImin). Among the most represented necrophagous species, Lucilia sericata (Diptera, Calliphoridae) is particularly interesting. It is regularly identified in samples, with a predominance in summer, and is commonly used by analysts of our entomology department (Institut de Recherche Criminelle de la Gendarmerie Nationale) to estimate the PMImin with the accumulated degree days (ADD) method. This method requires the mathematical lower thermal threshold to be known. This value dictates the quality of the applied ADD method but cannot be considered as fixed, especially when insect development occurs at temperatures close to the biological threshold. In such conditions, it is necessary to study the influence of such temperatures on development rate, as well as the consequences of estimating the period of first oviposition on cadavers, when using the ADD method. Seven replicate rearings were conducted at six different temperatures: 30 °C, 24 °C, 18 °C, 15 °C, 12 °C and 10 °C. Time of development and time of emergence were recorded. The effect of low temperature on the development cycle and the reliability of the ADD method under this entire temperature spectrum were studied using different linear regression models. Calculated durations of total insect time development and experimental rearing duration were then compared. A global linear model cannot be used on the whole temperature spectrum experienced by L. sericata without resulting in an overestimation at some temperatures. We found a combination of two linear regression models to be suitable for the estimation of the total development time, depending on the temperature experienced by L. sericata. This approach allowed us to obtain a variation lower than 2% at 12 °C and 10 °C between the calculated duration and experimental duration of development. In comparison, the results obtained with a global model show a variation higher than 3% at 12 °C and 10% at 10 °C.

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“…Most of the published data are focused on the middle temperature range (10–30 °C), and only a few studies have investigated the effect of high temperatures on L. sericata development (Grassberger & Reiter, ; Roe & Highley, ; Aubernon et al ., ). Based on the few available data, at high temperatures of 30–35 °C, the development rate slows or even decreases (Grassberger & Reiter, ; Gosselin et al ., ; Roe & Higley, ). Additionally, larvae bred at high temperature are usually smaller with lower probability to successfully develop into an adult fly (Grassberger & Reiter, ; Gosselin et al ., ).…”

Section: Discussionmentioning

confidence: 99%

“…Based on the few available data, at high temperatures of 30-35°C, the development rate slows or even decreases (Grassberger & Reiter, 2001;Gosselin et al, 2010;Roe & Higley, 2015). Additionally, larvae bred at high temperature are usually smaller with lower probability to successfully develop into an adult fly (Grassberger & Reiter, 2001;Gosselin et al, 2010). These high temperature effects are dependent on the critical thermal maximum of each species (Marchenko, 2001;Aubernon et al, 2016).…”

Section: Figmentioning

confidence: 99%

To eat or get heat: Behavioral trade‐offs between thermoregulation and feeding in gregarious necrophagous larvae

et al. 2017

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The thermoregulation behavior of Lucilia sericata larvae (Diptera: Calliphoridae), a necrophagous species that feeds on vertebrate cadavers, was investigated. These larvae require high heat incomes to develop, and can elevate temperatures by forming large aggregates. We hypothesized that L. sericata larvae should continue to feed at temperatures up to 38 °C, which can be reached inside larval masses. Thermal regulation behavior such as movement between a hot food spot and colder areas was also postulated. The hypotheses were tested by tracking for 1 h the activity of single, starved third instar larvae in a Petri dish containing 1 food spot (FS) that was heated to a constant temperature of 25 °C, 34 °C or 38 °C with an ambient temperature of 25 °C. The influence of previous conspecific activity in the food on larval behavior was also tested. The crops of larvae were dissected to monitor food content in the digestive systems. Based on relative crop measurements, larvae fed at all food temperatures, but temperature strongly affected larval behavior and kinematics. The total time spent by larvae in FS and the duration of each stay decreased at high FS temperature. Previous activity of conspecifics in the food slightly increased the time spent by larvae in FS and also decreased the average distance to FS. Therefore, necrophagous L. sericata larvae likely thermoregulate during normal feeding activities by adjusting to local fluctuations in temperature, particularly inside maggot masses. By maintaining a steady internal body temperature, larvae likely reduce their development time.

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Development Time Variability: Adaptation of Régnière’s Method to the Intrinsic Variability of Belgian Lucilia Sericata (Diptera, Calliphoridae) Population

Cited by 7 publications

References 22 publications

In Vitro Effects of Household Products on Calliphoridae Larvae Development: Implication for Forensic Entomology

In Vitro Effects of Household Products on Calliphoridae Larvae Development: Implication for Forensic Entomology

Effect of low temperature in the development cycle ofLucilia sericata(Meigen) (Diptera, Calliphoridae): implications for the minimum postmortem interval estimation

To eat or get heat: Behavioral trade‐offs between thermoregulation and feeding in gregarious necrophagous larvae

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