Effect of low temperature in the development cycle ofLucilia sericata(Meigen) (Diptera, Calliphoridae): implications for the minimum postmortem interval estimation

Abstract

Knowledge of necrophagous insects’ developmental data is necessary for the forensic entomologist to estimate a reliable minimum postmortem interval (PMImin). Among the most represented necrophagous species, Lucilia sericata (Diptera, Calliphoridae) is particularly interesting. It is regularly identified in samples, with a predominance in summer, and is commonly used by analysts of our entomology department (Institut de Recherche Criminelle de la Gendarmerie Nationale) to estimate the PMImin with the accumulated degree days (ADD) method. This method requires the mathematical lower thermal threshold to be known. This value dictates the quality of the applied ADD method but cannot be considered as fixed, especially when insect development occurs at temperatures close to the biological threshold. In such conditions, it is necessary to study the influence of such temperatures on development rate, as well as the consequences of estimating the period of first oviposition on cadavers, when using the ADD method. Seven replicate rearings were conducted at six different temperatures: 30 °C, 24 °C, 18 °C, 15 °C, 12 °C and 10 °C. Time of development and time of emergence were recorded. The effect of low temperature on the development cycle and the reliability of the ADD method under this entire temperature spectrum were studied using different linear regression models. Calculated durations of total insect time development and experimental rearing duration were then compared. A global linear model cannot be used on the whole temperature spectrum experienced by L. sericata without resulting in an overestimation at some temperatures. We found a combination of two linear regression models to be suitable for the estimation of the total development time, depending on the temperature experienced by L. sericata. This approach allowed us to obtain a variation lower than 2% at 12 °C and 10 °C between the calculated duration and experimental duration of development. In comparison, the results obtained with a global model show a variation higher than 3% at 12 °C and 10% at 10 °C.

Effect of increased ambient temperature on seasonal generation number in Lucilia sericata (Diptera, Calliphoridae)

“…It is based on the most fundamental studies and is geographically closest to the ecosystem we modeled -the vicinity of the Gubinikha meteorological station (Dnepropetrovsk region, Ukraine, 48.8096°N, 35.2591°E). We used 3 ADD and ADH values presented in the literature to calculate our assessment of ADH and ADD values changing throughout 2005-2020 (Table 1) (Marchenko, 2001;Cervantès et al, 2017;Wang et al, 2020). However, in the process of modelling the influence of increased temperature on the number of generations we have used ADH value provided by Wang et al, (2020).…”

New Insights Into Culturable and Unculturable Bacteria Across the Life History of Medicinal Maggots Lucilia sericata (Meigen) (Diptera: Calliphoridae)

“…Because of aforesaid nutritional ecology, L. sericata is at the forefront of applied biological sciences. The immature stages are ripened within the decomposing carrion or animal remains, which their developmental data can be exploited for the estimation of the time elapsed since death, denoted as postmortem interval (PMI) in the forensic entomology (Cervantès et al, 2018). Likewise, it is one of the facultative parasites of animals and humans causing wound myiasis worldwide (Francesconi and Lupi, 2012).…”

Estimation of accumulated degree hours-based post-mortem intervals in mammalian and avian model

“…The ADH was calculated for L. sericata life cycle in both cases by using the following formula: ADH=Time (Hrs. )×(Average temperature-Minimum development threshold temperature) and the reference developmental durations for L. sericata used in the study were obtained from Cervantes et al, (2017).…”