Development of wound healing scaffolds with precisely-triggered sequential release of therapeutic nanoparticles

Ahmad, Tauseef; McGrath, Sean; Sirafim, Catherine; Amaral, Rui; Soong, Shin-Loong; Sitram, Renuka; Turkistani, Shifa'a; Santarella, Francesco; Kearney, Cathal J.

doi:10.1039/d0bm01277g

Cited by 26 publications

(20 citation statements)

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“…Collagen wound dressings contain collagen blended with natural and synthetic polymers such as polyethylene oxide, poly (L-lactic acid), hyaluronic acid, elastin and silk fibroin, alginate, chitosan, etc [ 73 , 81 , 82 , 83 , 84 , 85 ]. These blended fabrications have incorporated other additives such as insulin [ 86 ], antibiotics [ 87 , 88 , 89 , 90 , 91 , 92 ] or gold nanoparticles [ 93 , 94 , 95 ] and have been tested mostly in in vitro studies or small animal models of wound healing. An evidence-based review of clinical studies on antibacterial integrated collagen wound dressings indicated that most studies were limited by small sample sizes and mixed chronic wound etiologies [ 46 ].…”

Section: Collagen Formats and Applications In Wound Healingmentioning

confidence: 99%

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Collagen in Wound Healing

2021

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Normal wound healing progresses through inflammatory, proliferative and remodeling phases in response to tissue injury. Collagen, a key component of the extracellular matrix, plays critical roles in the regulation of the phases of wound healing either in its native, fibrillar conformation or as soluble components in the wound milieu. Impairments in any of these phases stall the wound in a chronic, non-healing state that typically requires some form of intervention to guide the process back to completion. Key factors in the hostile environment of a chronic wound are persistent inflammation, increased destruction of ECM components caused by elevated metalloproteinases and other enzymes and improper activation of soluble mediators of the wound healing process. Collagen, being central in the regulation of several of these processes, has been utilized as an adjunct wound therapy to promote healing. In this work the significance of collagen in different biological processes relevant to wound healing are reviewed and a summary of the current literature on the use of collagen-based products in wound care is provided.

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Section: Collagen Formats and Applications In Wound Healingmentioning

confidence: 99%

“…Collagen is also used as a surface coating to enhance moisture retention and promote cell adhesion within scaffolds/matrices [ 73 , 81 , 82 , 83 , 84 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 ]. Water retention is important to keep the wound bed moist.…”

Section: Collagen Formats and Applications In Wound Healingmentioning

confidence: 99%

Collagen in Wound Healing

2021

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“…[23a,33b,38] Briefly, control collagen-glycosaminoglycan (CG) slurry was produced by blending bovine tendon collagen I (5 mg mL À1 with chondroitin-6-sulphate (GAG ¼ 0.04%) (Sigma-Aldrich, Germany) in 0.05 M acetic acid. [39] DFU and iDFU ECM was generated in T175 flasks, by seeding the cells at a concentration of 64 k cells cm À2 in differentiation media containing 100 μg mL À1 ascorbic acid (changed every 72 h) for 3 weeks. After washing in PBS, the 2D cell monolayer was scraped off the flask, centrifuged, and after supernatant removal, snap frozen in liquid nitrogen.…”

Section: Methodsmentioning

confidence: 99%

Personalized Scaffolds for Diabetic Foot Ulcer Healing Using Extracellular Matrix from Induced Pluripotent Stem‐Reprogrammed Patient Cells

Santarella

Amaral

Lemoine

et al. 2022

Advanced NanoBiomed Research

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Diabetic foot ulcers (DFUs) are chronic wounds sustained by pathological fibroblasts and aberrant extracellular matrix (ECM). Porous collagen‐based scaffolds (CS) have shown clinical promise for treating DFUs but may benefit from functional enhancements. Our previous work showed fibroblasts differentiated from induced pluripotent stem cells are an effective source of new ECM mimicking fetal matrix, which notably promotes scar‐free healing. Likewise, functionalizing CS with this rejuvenated ECM shows potential for DFU healing. Herein, an approach to DFU healing is demonstrated for the first time using biopsied cells from DFU patients, reprogramming those cells, and functionalizing CS with patient‐specific ECM as a personalized acellular tissue‐engineered scaffold. A two‐pronged approach is taken: 1) direct ECM blending into scaffold fabrication, and 2) seeding scaffolds with reprogrammed fibroblasts for ECM deposition followed by decellularization. The decellularization approach reduces cell number requirements and maintains naturally deposited ECM proteins. Both approaches show enhanced ECM deposition from DFU fibroblasts. Decellularized scaffolds additionally enhance glycosaminoglycan deposition and subsequent vascularization. Finally, reprogrammed ECM scaffolds from patient‐matched DFU fibroblasts outperform those from healthy fibroblasts in several metrics, suggesting ECM is in fact able to redirect resident pathological fibroblasts in DFUs toward healing, and a patient‐specific ECM signature may be beneficial.

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“…Antimicrobial agents may even be able to be spun into scaffolds or be delivered ondemand via nanoparticles. 81,82 Researchers are also interested in combining the regenerative potential of stem cells and 3D bioprinting to construct vascularized skin grafts which would ensure the perfusion necessary for the rapid take and long-term survival of grafts. 83,84…”

Section: New Frontiers In Researchmentioning

confidence: 99%

Comparison of Skin Substitutes for Acute and Chronic Wound Management

et al. 2021

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Chronic and acute wounds, such as diabetic foot ulcers and burns, respectively, can be difficult to treat, especially when autologous skin transplantations are unavailable. Skin substitutes can be used as a treatment alternative by providing the structural elements and growth factors necessary for reepithelialization and revascularization from a nonautologous source. As of 2020, there are 76 commercially available skin substitute products; this article provides a review of the relevant literature related to the major categories of skin substitutes available.

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Development of wound healing scaffolds with precisely-triggered sequential release of therapeutic nanoparticles

Abstract: Natural bioactive cue profiles are generally transient with cues switching on/off to coordinate successful outcomes. Dysregulation of these sequences typically leads to disease. Successful wound healing, for example, should progress...

Cited by 26 publications

References 40 publications

Collagen in Wound Healing

Collagen in Wound Healing

Personalized Scaffolds for Diabetic Foot Ulcer Healing Using Extracellular Matrix from Induced Pluripotent Stem‐Reprogrammed Patient Cells

Comparison of Skin Substitutes for Acute and Chronic Wound Management

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