Development of vitrified-warmed mouse embryos co-cultured with polarized or non-polarized uterine epithelial cells using sequential culture media

Azadbakht, Mehri; Valojerdi, Mojtaba Rezazadeh

doi:10.1007/s10815-008-9231-4

Cited by 7 publications

(5 citation statements)

References 49 publications

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“…Sperm suspensions were capacitated for 1.5 h in T6 medium supplemented with 5 mg/ml BSA. T6 medium composition was as described previously [11]. MII oocytes were collected from different groups and MII oocytes (n 010) were inseminated into 50 uL droplets of T6 medium supplemented with 15 mg/ ml BSA and a final motile sperm concentration of 1-2×10 6 / ml then incubated for 4-6 h. At the end of this period, inseminated oocytes (n010) were cultured in 50 uL microdroplets of T6 medium supplemented with 5 mg/ml BSA for 120 h. The percentage of embryos developing to the blastocyte stages was determined.…”

Section: In Vitro Fertilizationmentioning

confidence: 99%

The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

Talebi

Zavareh

Kashani

et al. 2012

J Assist Reprod Genet

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Purpose This study was designed to investigate the effect of alpha-lipoic acid (ALA) on reactive oxygen species (ROS) production, total antioxidant capacity (TAC) and developmental competence of cultured pre-antral follicles derived from mouse ovarian tissue. Methods Pre-antral follicles were isolated from immature mouse ovaries and were cultured in α-minimal essential medium supplemented with different concentrations (0, 50, 100, 250 and 500 uM) of ALA. Follicular growth, oocyte maturation and embryo development were evaluated. Separately, ROS and TAC were measured after 0, 24, 48, 72 and 96 h of culture with spectrofluorometery and ferric reducing/antioxidant power (FRAP) assay, respectively. Results In the presence of 100 uM ALA, developmental rates of follicles, oocytes and embryos were significantly higher than other groups (p<0.05). At 96 h after culture, a decrease in ROS and an increase in TAC were observed in ALA group compared to control group (p<0.05). Conclusion ALA (100 uM) improves the in vitro development of follicles. This effect may be mediated by decreasing ROS concentration and increasing follicular TAC level during the culture period.

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Section: In Vitro Fertilizationmentioning

confidence: 99%

The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

Talebi

Zavareh

Kashani

et al. 2012

J Assist Reprod Genet

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“…The high success rate of vitrification minimizes the period of embryo exposure to cryoprotectants such as ethylene glycol (EG) and dimethyl sulfoxide (DMSO); these reduce osmotic stress and prevent formation of ice crystals [3]. However, vitrification also has harmful effects on survival and development of embryos [4,5]. Thus, low rate of preimplantation embryo development is the main problem due to creation of reactive oxygen species (ROS) during the process [6].…”

Section: Introductionmentioning

confidence: 99%

Melatonin modulates the expression of BCL-xl and improve the development of vitrified embryos obtained by IVF in mice

Dehghani-Mohammadabadi

Salehi

Farifteh

et al. 2014

J Assist Reprod Genet

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“…Embryos were defined "morphologically survived", if they possessed an intact zona pellucida, blastomeres, and refractive cytoplasm (Boonkusol et al, 2006;Mamo et al, 2006;Azadbakht and Rezazadeh Valojerdi, 2008). Following the thawing and cryoprotectant removal steps, four-cell embryos in 100 μl of sterilized KSOM +AA (Millipore, Bedford, USA) supplemented with 4 mg/ml BSA were incubated under mineral oil with the availability of 5% (v/v) CO 2 , 5% (v/v) O 2 , and 90% (v/v) N 2 for 1 h at 37°C.…”

Section: Definition Of Morphological Survivalmentioning

confidence: 99%

Transcript analysis of Heat shock protein 72 and protein 53 of 4-cell mouse embryos following Cryotop vitrification

Habibi¹,

Farrokhi²,

Silva³

et al. 2013

CZECH J ANIM SCI

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ABSTRACT:The effects of two different concentrations of cryoprotectants on survival and developmental capacity of four-cell mouse embryos were compared by Cryotop vitrification to demonstrate that lower concentrations provide the same results as higher previously reported concentrations with lesser negative molecular impact on embryo cells. For this latest, embryos were compared via transcript analyses of Heat shock protein 72 (Hsp72) and protein 53 (p53). Four-cell embryos were obtained from superovulated female mice and randomly assigned to one of three following groups: (i) control (non-vitrified), (ii) vit 1 (15% v/v: 7.5% ethylene glycol (EG) and 7.5% dimethyl sulfoxide (DMSO), and (iii) vit 2 (30% v/v: 15% EG + 15% DMSO). The cells vitrified by Cryotop were thawed and side-by-side to the control group divided into two parts: one part was used to analyze the morphological traits, survival rate, and embryo cleavage ability to form blastocysts, and the other part was examined for changes in transcript levels of Hsp72 (Hspa1a + Hspa1b), p53, and Hprt1 (reference gene) by quantitative Real-Time polymerase chain reaction (qPCR). The results were analyzed by One Way Analysis of Variance and the mean values compared with LSD (P < 0.05). The relative expression of p53 in vit 2 (30% v/v) was significantly higher than in vit 1 (15% v/v) and in vit 1 it was higher than in the control. The relative expression of Hsp72 was the same in vit 1 and vit 2 and significantly higher than in the control. The survival, cleavage, and blastocyst rates were the same for both vitrification treatments and significantly lower than in the control group. The up-regulations of Hsp72 and p53 following vitrification were suggestive of imposed heat shock, cold stress, and DNA damage to the mouse 4-cell embryos.

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Development of vitrified-warmed mouse embryos co-cultured with polarized or non-polarized uterine epithelial cells using sequential culture media

Abstract: Co-culture system involving polarized uterine epithelial cells and sequential culture media is a promising method for the improvement of vitrified-warmed mouse embryo development.

Cited by 7 publications

References 49 publications

The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

Melatonin modulates the expression of BCL-xl and improve the development of vitrified embryos obtained by IVF in mice

Transcript analysis of Heat shock protein 72 and protein 53 of 4-cell mouse embryos following Cryotop vitrification

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