Development of urea biosensor using non-covalent complexes of urease with aldehyde derivative of PEG and analysis on serum samples

Vardar, Gokay; Attar, Azade; Yapaöz, Melda Altıkatoğlu

doi:10.1080/10826068.2019.1630650

Cited by 4 publications

(3 citation statements)

References 36 publications

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“…The activity of urease enzyme on PEG‐aldehyde complex was determined by Berthelot assay. This fast and efficient non‐covalent, immobilised urea biosensor complex is beneficial in enzyme technology [ 59 ]. A mobile sensor of urea for use under rapid flow conditions was manufactured by vapour deposition using PTFE which is a porous membranes covered with parylene functionalised with amine and parylene‐A.…”

Section: Classification Of Urea Biosensorsmentioning

confidence: 99%

Recent advancements in urea biosensors for biomedical applications

Singh

Sharma

Singh

2021

IET Nanobiotechnology

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The quick progress in health care technology as a recurrent measurement of biochemical factors such as blood components leads to advance development and growth in biosensor technology necessary for effectual patient concern. The review wok of authors present a concise information and brief discussion on the development made in the progress of potentiometric, field effect transistor, graphene, electrochemical, optical, polymeric, nanoparticles and nanocomposites based urea biosensors in the past two decades. The work of authors is also centred on different procedures/methods for detection of urea by using amperometric, potentiometric, conductometric and optical processes, where graphene, polymer etc. are utilised as an immobilised material for the fabrication of biosensors. Further, a comparative revision has been accomplished on various procedures of urea analysis using different materials-based biosensors, and it discloses that electrochemical and potentiometric biosensor is the most promise one among all, in terms of rapid response time, extensive shelf life and resourceful design.This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Section: Classification Of Urea Biosensorsmentioning

confidence: 99%

Recent advancements in urea biosensors for biomedical applications

Singh

Sharma

Singh

2021

IET Nanobiotechnology

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“…The urease enzyme is a hydrolase class enzyme with a molecular weight of approximately 480 kDa that catalyzes the hydrolysis of urea to ammonia and carbon dioxide following eq It was reported that the urease enzyme can be obtained from a wide variety of organisms including plants, fungi, and bacteria. − The catalytic activity of this enzyme for urea, the end product of the nitrogen cycle, makes it important . The hydrolysis of urea to ammonia and carbon dioxide increases pH, which has negative agronomic and public health repercussions .…”

Section: Introductionmentioning

confidence: 99%

“…13−15 The catalytic activity of this enzyme for urea, the end product of the nitrogen cycle, makes it important. 16 The hydrolysis of urea to ammonia and carbon dioxide increases pH, which has negative agronomic and public health repercussions. 17 Urease assays are required in pharmaceutical, medical, agricultural, and environmental applications since urea may be detected in biological fluids such as blood and urine.…”

Section: ■ Introductionmentioning

confidence: 99%

Urease-Immobilized PEI Cryogels for the Enzymatic Hydrolysis of Urea and Carbon Dioxide Uptake

Demirci

Şahiner

2022

Ind. Eng. Chem. Res.

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Here, the immobilization of the urease enzyme within polyethyleneimine (PEI) cryogels (Urease@PEI cryogel) is reported during the synthesis via the entrapment method. The immobilization yield of the enzymes within PEI cryogels was determined as >85%, and the activity% of the prepared Urease@ PEI cryogel systems was calculated as >90% with respect to equal amounts of native urease enzyme. The working stability of Urease@PEI cryogel systems was observed to be better than native urease enzymes in the pH range of 5.0−9.0 and the temperature range of 15−45 °C. Furthermore, the immobilization of urease enzyme within PEI cryogels afforded great performance for use in continuous systems with more than 70% activity. Moreover, Urease@PEI cryogel systems preserved more than 50% activity even after 20 consecutive uses and showed superior storage stability, e.g., storage at 25 °C for 10 days resulted in >50% activity. Most importantly, the possible utilization of Urease@PEI cryogel systems for CO 2 adsorption, which is the byproduct of the enzymatic reaction of urea, from the reaction media was investigated. The CO 2 capture capability was calculated as 0.42, 0.27, and 0.11 cm 3 /g at 1 bar and 25 °C

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