Development of Tripolymeric Triaxial Electrospun Fibrous Matrices for Dual Drug Delivery Applications

Abstract

Since the first work by Laurencin and colleagues on the development of polymeric electrospinning for biomedical purposes, the use of electrospinning technology has found broad applications in such areas of tissue regeneration and drug delivery. More recently, coaxial electrospinning has emerged as an important technique to develop scaffolds for regenerative engineering incorporated with drug(s). However, the addition of a softer core layer leads to a reduction in mechanical properties. Here, novel robust tripolymeric triaxially electrospun fibrous scaffolds were developed with a polycaprolactone (PCL) (core layer), a 50:50 poly (lactic-co-glycolic acid) (PLGA) (sheath layer) and a gelatin (intermediate layer) with a dual drug delivery capability was developed through modified electrospinning. A sharp increase in elastic modulus after the incorporation of PCL in the core of the triaxial fibers in comparison with uniaxial PLGA (50:50) and coaxial PLGA (50:50) (sheath)-gelatin (core) fibers was observed. Thermal analysis of the fibrous scaffolds revealed an interaction between the core-intermediate and sheath-intermediate layers of the triaxial fibers contributing to the higher tensile modulus. A simultaneous dual release of model small molecule Rhodamine B (RhB) and model protein Fluorescein isothiocynate (FITC) Bovine Serum Albumin (BSA) conjugate incorporated in the sheath and intermediate layers of triaxial fibers was achieved. The tripolymeric, triaxial electrospun systems were seen to be ideal for the support of mesenchymal stem cell growth, as shrinkage of fibers normally found with conventional electrospun systems was minimized. These tripolymeric triaxial electrospun fibers that are biomechanically competent, biocompatible, and capable of dual drug release are designed for regenerative engineering and drug delivery applications.

Preparation and modeling of three‐layered PCL/PLGA/PCL fibrous scaffolds for prolonged drug release

“…Previous research with multi-layer scaffolds has developed controlled release materials and methods where drug release begins immediately after the first day [49][50][51] . Our unique scaffold system can be used for localized drug release and potential post-surgical cancer treatment twenty-one days after surgery.…”

Preparation and characterization of amnion hydrogel and its synergistic effect with adipose derived stem cells towards IL1β activated chondrocytes

“…ADSCs were isolated from 6–8 week-old Sprague Dawley (SD) rats around 250 g as reported in ur previous study 53 in accordance with the experimental guidelines and regulations approved by University of Connecticut Health Center Institutional Animal Care and Use Committee (IACUC) approved protocol. Additionally, ADSC isolation was done following the institutional IACUC approved protocol.…”

“…The threshold cycle values of target genes were standardized against GAPDH expression and normalized to the expression in the control culture. The fold change in expression was calculated using the ΔΔCt comparative threshold cycle method 53 .…”

Structural design toward functional materials by electrospinning: A review

“…Electrospun nanofibers with porous, core-shell, hollow and Janus structure have been widely used in the field of catalysis (170). Porous fibers not only provide a larger oxygen uptake area but also capture more photons.…”