Development of Trichoderma sp. formulations in encapsulated granules (CG) and evaluation of conidia shelf-life

Locatelli, Gabriel Olivo; Santos, Glauber Eduardo de Oliveira; Botelho, P. S.; Finkler, Christine Lamenha Luna; Bueno, Luciano A.

doi:10.1016/j.biocontrol.2017.08.020

Cited by 56 publications

(40 citation statements)

References 25 publications

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“…T. koningii, T. viride and T. harzianum were purchased from the Guangdong Culture Collection Center (GCCC), and T. longibrachiatum, T. hamatum, T. atroviride, and T. spirale were purchased from the China General Microbiological Culture Collection Center (CGMCC) ( Table 1). These fungi were cultured in the potato dextrose agar (PDA) medium in an environmental chamber set at 28 ± 1 • C. After conidia grew on the surface, 5 mL sterile distilled water was added into the PDA medium to obtain the conidial suspensions, which were then transferred to a 500 mL Erlenmeyer flask containing rice medium (50 mL distilled water and 50 g dried rice sterilized at 121 • C for 20 min [31,32]). After 7-10 days, 200 mL sterile distilled water was added into the rice medium, and the flask was shaken for 5 min using a vortexer.…”

Section: Trichoderma Fungimentioning

confidence: 99%

The Effects of Trichoderma Fungi on the Tunneling, Aggregation, and Colony-Initiation Preferences of Black-Winged Subterranean Termites, Odontotermes formosanus (Blattodea: Termitidae)

Xiong

Cai

Chen

et al. 2019

Forests

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The black-winged subterranean termite, Odontotermes formosanus Shiraki, is a severe pest of plantations and forests in China. This termite cultures symbiotic Termitomyces in the fungal combs, which are challenged by antagonistic microbes such as Trichoderma fungi. In a previous study we showed that O. formosanus workers made significantly fewer tunnels in sand containing commercially formulated conidia of Trichoderma viride Pers. ex Fries compared with untreated sand. Herein, we hypothesize that fungi in the genus Trichoderma exert repellent effects on O. formosanus. Different choice tests were conducted to evaluate the tunneling and aggregation behaviors of O. formosanus workers reacting to sand/soil containing the unformulated conidia of seven Trichoderma fungi (Trichoderma longibrachiatum Rifai, Trichoderma koningii Oud., Trichoderma harzianum Rifai, Trichoderma hamatum (Bon.) Bain, Trichoderma atroviride Karsten, Trichoderma spirale Indira and Kamala, and T. viride). We also investigated the colony-initiation preference of paired O. formosanus adults to soil treated with Trichoderma conidia (T. koningii or T. longibrachiatum) versus untreated soil. Tunneling-choice tests showed that sand containing conidia of nearly all Trichoderma fungi tested (except T. harzianum) significantly decreased tunneling activity in O. formosanus workers compared with untreated sand. Aggregation-choice test showed that T. koningii, T. atroviride and T. spirale repelled O. formosanus workers, whereas T. longibrachiatum and T. hamatum attracted termites. There was no significant difference in proportions of paired adults that stayed and laid eggs in the soil blocks treated with conidia of Trichoderma fungi and untreated ones. Our study showed that Trichoderma fungi generally repelled tunneling in O. formosanus, but may exert varied effects on aggregation preference by workers.

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Section: Trichoderma Fungimentioning

confidence: 99%

The Effects of Trichoderma Fungi on the Tunneling, Aggregation, and Colony-Initiation Preferences of Black-Winged Subterranean Termites, Odontotermes formosanus (Blattodea: Termitidae)

Xiong

Cai

Chen

et al. 2019

Forests

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“…However, field environmental conditions are still one of the main limitations for the use of biocontrol agents (Bashan et al 2014). Parameters such as ultraviolet radiation (UVR) (Costa et al 2016), relative humidity (Swaminathan et al 2016), temperature (Domingues et al 2016, and storage conditions (Locatelli et al 2018) could have a negative influence on inoculum viability. Using appropriate formulations is a way to overcome this problem, which create microclimates that protect spores from adverse environmental conditions (Doni et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Formulation of Trichoderma asperellum TV190 for biological control of Rhizoctonia solani on corn seedlings

Herrera

Valbuena

Pavone-Maniscalco

2020

Egypt J Biol Pest Control

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Environmental conditions affect biocontrol agents in a field, being appropriate formulations an alternative to overcome this problem. Formulations based on Trichoderma asperellum TV190 were prepared by emulsified mineral or vegetable oils, which protected spores from ultraviolet radiation, showing greater viability of 37-43% (mineral) and 56-63% (vegetable) than the control (8-12%). These formulations improved an antagonism of T. asperellum on Rhizoctonia solani under greenhouse conditions, reducing infected corn seedlings by 72% (mineral) and 59% (vegetable). Necrotic spot size was reduced by 90.04% (mineral) and 87.29% (vegetable). A granular formulation, prepared with degreased corn germ and T. asperellum spores, protected the corn seedlings from R. solani under greenhouse conditions, with 73% reduction of infected plants and 93% reduction of necrotic spot size. Both granular and liquid formulations were able to improve T. asperellum antagonism, suggesting that these formulations could be included in agricultural pest control strategies.

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“…Controlled-release systems can decrease the release rate of entrapped substances, reduce the amount of leakage, and allow the entrapped substances to last longer in the environment. Starch-alginate formulations have been demonstrated to be utilized in the encapsulation of proteins [13], antioxidants [14], biocontrol Trichoderma [15], and the controlled release of agrochemicals such as thiram [16], soproturon, and imidacloprid [17]. In a previous work, we developed starch-alginate-based formulations for the simultaneous encapsulation of biocontrol fungus and metalaxyl, and the release rate was observed to slow down with the addition of kaolin and rice husk powder [18].…”

Section: Introductionmentioning

confidence: 99%

Thermosensitive Hydrogel for Encapsulation and Controlled Release of Biocontrol Agents to Prevent Peanut Aflatoxin Contamination

et al. 2020

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Starch, alginate, and poly(N-isopropylacrylamide) (PNIPAAm) were combined to prepare a semi-interpenetrating network (IPN) hydrogel with temperature sensitivity. Calcium chloride was used as cross-linking agent, the non-toxigenic Aspergillus flavus spores were successfully encapsulated as biocontrol agents by the method of ionic gelation. Characterization of the hydrogel was performed by Fourier-transform infrared spectroscopy (FTIR), scanning electron micrograph (SEM), and thermogravimetry analysis (TGA). Formulation characteristics, such as entrapment efficiency, beads size, swelling behavior, and rheological properties were evaluated. The optical and rheological measurements indicated that the lower critical solution temperature (LCST) of the samples was about 29–30 °C. TGA results demonstrated that the addition of kaolin could improve the thermal stability of the semi-IPN hydrogel. Morphological analysis showed a porous honeycomb structure on the surface of the beads. According to the release properties of the beads, the semi-IPN hydrogel beads containing kaolin not only have the effect of slow release before peanut flowering, but they also can rapidly release biocontrol agents after flowering begins. The early flowering stage of the peanut is the critical moment to apply biocontrol agents. Temperature-sensitive hydrogel beads containing kaolin could be considered as carriers of biocontrol agents for the control of aflatoxin in peanuts.

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Development of Trichoderma sp. formulations in encapsulated granules (CG) and evaluation of conidia shelf-life

Cited by 56 publications

References 25 publications

The Effects of Trichoderma Fungi on the Tunneling, Aggregation, and Colony-Initiation Preferences of Black-Winged Subterranean Termites, Odontotermes formosanus (Blattodea: Termitidae)

The Effects of Trichoderma Fungi on the Tunneling, Aggregation, and Colony-Initiation Preferences of Black-Winged Subterranean Termites, Odontotermes formosanus (Blattodea: Termitidae)

Formulation of Trichoderma asperellum TV190 for biological control of Rhizoctonia solani on corn seedlings

Thermosensitive Hydrogel for Encapsulation and Controlled Release of Biocontrol Agents to Prevent Peanut Aflatoxin Contamination

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