Development of the Vaginal Epithelium Showing Estrogen-Independent Proliferation and Cornification in Neonatally Androgenized Mice

Mitotic activity in vaginal epithelium in neonatally androgenized mice following estrogen administration.

“…Moreover, the urethral side of the distal part of vagina of such mice, the mitotic activity was always lower than in the antiurethral side. These findings are in harmony with the previous findings that the vaginal epithelium failed to mucify following treatment with E2 plus progesterone in neonatally androgenized mice (Ohta and Iguchi, 1976).…”

“…The epithelium lining the cervical part was stratified with or without overlying mucous cells. The epithelium of the distal part consisted of 4-7 layers of cells without any specialized superficial layer, as reported previously (Iguchi and Takasugi, 1976 ;Ohta and Iguchi, 1976) . The uterine epithelium of the ovariectomized control mice was composed of a single layer of low columnar cells.…”

Alkaline phosphatase activity in vagina of mice following neonatal administration of estrogen or androgen.

“…On the basis of the previous (Iguchi et atl., 1976;Ohta and Iguchi, 1976) and the present findings, it is evident that in the vaginal epithelium of the steroid-treated mice, AP activity increases with the elevation of the mitotic rate as has already been suggested by Mori (1967).…”

“…However, careful studies on histogenesis and mitotic activity of this type of vaginal epithelium Ohta and Iguchi, 1976) demonstrated differences in process leading to the estrogen-independent changes between animals given estrogen and androgen neonatally .…”

Effects of Neonatal Injections of Androgen on Induction of Permanent Changes in the Mouse Vaginal Epithelium by Subsequent Estrogen Treatment

“…On the basis of the findings that early development of the permanently affected vaginal epithelium is markedly different between the estrogenized and androgenized mice, Ohta and Iguchi (1976) and Iguchi and Ohta (1980) have suggested that the mechanism involved in the induction of the permanent vaginal changes may be different between the two types of neonatal steroid treatment.…”