Development of the plant-derived peptide lunasin as an anticancer agent

Vuyyuri, Saleha B.; Shidal, Chris; Davis, Keith

doi:10.1016/j.coph.2018.04.006

Cited by 22 publications

(18 citation statements)

References 49 publications

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“…Lunasin is a forty-three amino acid peptide originally isolated from soybeans. It features a unique amino acid sequence containing an arginine-glycine-aspartic acid cell adhesion motif and a polyaspartic acid tail on its carboxylic acid end [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ]. The internalization of lunasin in macrophages is primarily mediated by endocytic mechanisms that involve integrin signaling, clathrin-coated structures, and micropinosomes.…”

Section: Introductionmentioning

confidence: 99%

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Potential Health Benefits Associated with Lunasin Concentration in Dietary Supplements and Lunasin-Enriched Soy Extract

et al. 2021

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Lunasin has demonstrated antioxidative, anti-inflammatory, and chemopreventive properties. The objectives were to evaluate the concentration of lunasin in different lunasin-based commercial dietary supplements, to produce a lunasin-enriched soy extract (LESE) using a two-step pilot-plant-based ultrafiltration process, and to evaluate their biological potential in vitro. LESE was produced using 30 and 1 kDa membranes in a custom-made ultrafiltration skid. Lunasin was quantified in eight products and LESE. Lunasin concentrations of the lunasin-based products ranged from 9.2 ± 0.6 to 25.7 ± 1.1 mg lunasin/g protein. The LESE extract contained 58.2 mg lunasin/g protein, up to 6.3-fold higher lunasin enrichment than lunasin-based dietary supplements. Antioxidant capacity ranged from 121.5 mmol Trolox equivalents (TE)/g in Now® Kids to 354.4 mmol TE/g in LESE. Histone acetyltransferase (HAT) inhibition ranged from 5.3% on Soy Sentials® to 38.3% on synthetic lunasin. ORAC and lunasin concentrations were positively correlated, and HAT and lunasin concentrations were negatively correlated (p < 0.05). Melanoma B16-F10 and A375 cells treated with lunasin showed dose-dependent inhibitory potential (IC50 equivalent to 330 and 370 μM lunasin, respectively). Lunasin showed protein kinase B expression (57 ± 14%) compared to the control (100%) in B16-F10. Lunasin concentration found in commercial products and lunasin-enriched soy extract could exert benefits to consumers.

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Section: Introductionmentioning

confidence: 99%

“…Lunasin exhibits different biological and chemopreventive properties including anti-inflammatory, anticarcinogenic, antioxidant and immune-modulating properties, anti-atherosclerosis, and osteoclastogenesis inhibition potential [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Potential Health Benefits Associated with Lunasin Concentration in Dietary Supplements and Lunasin-Enriched Soy Extract

et al. 2021

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“…Different forms of soybean have been evaluated and have shown controversial results; positive effects against the development of CAC or tumors have been reported, reaching a mean reduction of 56% in the frequency of crypts and 88% in the decrease in tumors [ 58 , 59 , 60 , 61 , 62 , 63 ]; however, negative results have also been obtained [ 64 , 65 ]. Regarding positive reports, Vuyyuri et al [ 66 ] suggested the involvement of the bioactive peptide lunasin as a main participant because of its multiple functional domains that may modulate gene expression through different mechanisms. Chickpea is another studied legume.…”

Section: Discussionmentioning

confidence: 99%

Phaseolin, a Protein from the Seed of Phaseolus vulgaris, Has Antioxidant, Antigenotoxic, and Chemopreventive Properties

et al. 2021

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The present report was designed to determine the antioxidant and antigenotoxic effects of phaseolin (isolated from Phaseolus vulgaris) against mouse colon and liver damage induced by azoxymethane (AOM) and its colon chemopreventive effect. Eight groups with 12 mice each were utilized for an eight-week experiment: the control group was intragastrically (ig) administered 0.9% saline solution; the positive control group was intraperitoneally (ip) injected with 7.5 mg/kg AOM twice a week (weeks three and four of the experiment); three groups were ig administered each day with phaseolin (40, 200, and 400 mg/kg); and three groups were ig administered phaseolin daily (40, 200, and 400 mg/kg) plus 7.5 mg/kg AOM twice a week in weeks three and four of the experiment. The results showed that phaseolin did not produce oxidative stress, DNA damage, or aberrant crypts; in contrast, 100% inhibition of lipoperoxidation, protein oxidation, and nitrites induction generated by AOM was found in both organs, and DPPH radical capture occurred. The two highest phaseolin doses reduced DNA damage induced by AOM in both organs by more than 90% and reduced the AOM-induced aberrant crypts by 84%. Therefore, our study demonstrated the strong in vivo antioxidant, antigenotoxic, and chemopreventive potential of phaseolin.

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“…In the highly metastatic KM12L4 cell line, Dia and de Mejia demonstrated that lunasin is able to internalize into the cell and sit within the nucleus, to modify the expression of human extracellular matrix and cell adhesion genes by binding to α 5 β 1 integrin, and also to inhibit the FAK/ERK/NF-κB signaling pathway [16,18]. Indeed, peptides containing the RGD-motif can bind integrins and block their signaling pathways involved in cell adhesion, invasion and extracellular matrix components, mechanisms by which lunasin has recently shown inhibitory and anti-metastatic effects in some cancer models [15,50]. Studies have found that lunasin inhibits non small cell lung cancer cell proliferation acting as antagonist of αv integrin and histone acetylation modulatory agent [32,51].…”

Section: Effect Of Lunasin On Cell Cycle Progression Of Crc Cellsmentioning

confidence: 99%

Inhibitory Effects of Peptide Lunasin in Colorectal Cancer HCT-116 Cells and Their Tumorsphere-Derived Subpopulation

Fernández-Tomé

Han

et al. 2020

IJMS

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The involvement of cancer stem-like cells (CSC) in the tumor pathogenesis has profound implications for cancer therapy and chemoprevention. Lunasin is a bioactive peptide from soybean and other vegetal sources with proven protective activities against cancer and other chronic diseases. The present study focused on the cytotoxic effect of peptide lunasin in colorectal cancer HCT-116 cells, both the bulk tumor and the CSC subpopulations. Lunasin inhibited the proliferation and the tumorsphere-forming capacity of HCT-116 cells. Flow cytometry results demonstrated that the inhibitory effects were related to apoptosis induction and cell cycle-arrest at G1 phase. Moreover, lunasin caused an increase in the sub-GO/G1 phase of bulk tumor cells, linked to the apoptotic events found. Immunoblotting analysis further showed that lunasin induced apoptosis through activation of caspase-3 and cleavage of PARP, and could modulate cell cycle progress through the cyclin-dependent kinase inhibitor p21. Together, these results provide new evidence on the chemopreventive activity of peptide lunasin on colorectal cancer by modulating both the parental and the tumorsphere-derived subsets of HCT-116 cells.

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Development of the plant-derived peptide lunasin as an anticancer agent

Cited by 22 publications

References 49 publications

Potential Health Benefits Associated with Lunasin Concentration in Dietary Supplements and Lunasin-Enriched Soy Extract

Potential Health Benefits Associated with Lunasin Concentration in Dietary Supplements and Lunasin-Enriched Soy Extract

Phaseolin, a Protein from the Seed of Phaseolus vulgaris, Has Antioxidant, Antigenotoxic, and Chemopreventive Properties

Inhibitory Effects of Peptide Lunasin in Colorectal Cancer HCT-116 Cells and Their Tumorsphere-Derived Subpopulation

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