Development of the male reproductive system and sexual transformation in the nematode Caenorhabditis elegans

Klass, Michael; Wolf, Nurit; Hirsh, David

doi:10.1016/0012-1606(76)90002-6

Cited by 121 publications

(41 citation statements)

References 14 publications

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“…LGI, dpy-5(e6l); smg-1(e1228) (Hodgkin et al, 1989); glp-4(bn2ts) (Beanan and Strome, 1992) LGII, bli-2(e768); tra-2(b202ts) (Klass et al, 1976) LGIII, unc-32(el89); fem-2(b245ts) (Kimble et al, 1984); glp-1(e2144ts) (Priess et al, 1987) LGIV, unc-5(e53); fem-i (el991) (Doniach and Hodgkin, 1984); feml(e2044) (Hodgkin, 1986); fem-l(e2382) (Spence et al, 1990); unc-24(e138); mor-2(e1125);fem-3(e1996) (Hodgkin, 1986); fem-3(q20 gfts) (Barton et al, 1987);dpy-20(el282ts) LGV, dpy-ll(e224); him-5(ei490) LGX, lon-2(e678) Standard methods of culture and genetic crossing of C. elegans were used (Sulston and Hodgkin, 1988), except that MYOB agar (Church et al, 1995) was used in place of NG agar in some experiments.…”

Section: Caenorhabditis Elegans Strains and Culture Methodsmentioning

confidence: 99%

Post-transcriptional regulation of sex determination in Caenorhabditis elegans: widespread expression of the sex-determining gene fem-1 in both sexes.

Gaudet

VanderElst

Spence

1996

MBoC

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The fem-1 gene of C. elegans is one of three genes required for all aspects of male development in the nematode. Current models of sex determination propose that the products of the fem genes act in a novel signal-transduction pathway and that their activity is regulated primarily at the post-translational level in somatic tissues. We analyzed the expression of fem-1 to determine whether it revealed any additional levels of regulation. Both XX hermaphrodites and XO males express fem-1 at approximately constant levels throughout development. Somatic tissues in hermaphrodites adopt female fates, but they nonetheless expressfem-1 mRNA and FEM-1 protein, suggesting that the regulation of fem-i activity is post-transcriptional and probably post-translational. A compact promoter directs functional expression of fem-1 transgenes, as assayed by their masculinizing activity infem-i mutants. Activity also requires any two or more introns, suggesting that splicing may enhance fem-1 expression. The minimal noncoding sequences required for activity of fem-1 transgenes suffice to direct expression of a fem-i::lacZ reporter gene in all somatic tissues in both sexes. Many fem-1 transgenes, including those that rescue male somatic development in fem-1 mutants, paradoxically feminize the germline. We suggest that they do so by interfering with the germline expression of the endogenous fem-1 gene.

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Section: Caenorhabditis Elegans Strains and Culture Methodsmentioning

confidence: 99%

Post-transcriptional regulation of sex determination in Caenorhabditis elegans: widespread expression of the sex-determining gene fem-1 in both sexes.

Gaudet

VanderElst

Spence

1996

MBoC

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“…Worms are 98% wild-type (hermaphrodite, with eggs in the adult stage) at 15C, but develop as 6 phenotypic males (without eggs and with a tail fan) at 25C (16). Although TRA-2 is expressed in both sexes, its function is blocked in males through binding of the male-specific HER-1 ligand.…”

Section: Dh202 (B202)mentioning

confidence: 99%

Effects of radio-frequency fields on bacterial cell membranes and nematode temperature-sensitive mutants

Güven¹,

Güven²,

Dawe³

et al. 2006

Enzyme and Microbial Technology

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A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription. suggestions that RF power is dissipated mainly in the plasma membrane of cells. However, these phenotype shifts are no longer seen when microwave treatment is applied at 21C in a modified exposure apparatus that minimises the temperature difference between sham and exposed conditions. Like other biological effects attributed to microwaves in the C. elegans system, phenotype shifts in ts mutants appear to be an artefact caused by very slight heating.

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“…We are studying the stage-specific regulation of migration in the linker cell (LC), an individual leader cell whose migration defines the shape of the developing male gonad and ultimately connects the gonad to the cloaca, enabling sperm release (Kimble and Hirsh, 1979;Klass et al, 1976). The LC clearly requires regulation at different stages as it navigates a complex trajectory during three larval stages (L2-L4) of development.…”

Section: Introductionmentioning

confidence: 99%

The C. elegans tailless/Tlx homolog nhr-67 regulates a stage-specific program of linker cell migration in male gonadogenesis

Kato

Sternberg

2009

Development

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Cell migration is a common event during organogenesis, yet little is known about how migration is temporally coordinated with organ development. We are investigating stage-specific programs of cell migration using the linker cell (LC), a migratory cell crucial for male gonadogenesis of C. elegans. During the L3 and L4 larval stages of wild-type males, the LC undergoes changes in its position along the migratory route, in transcriptional regulation of the unc-5 netrin receptor and zmp-1 zinc matrix metalloprotease, and in cell morphology. We have identified the tailless homolog nhr-67 as a cell-autonomous, stage-specific regulator of timing in LC migration programs. In nhr-67-deficient animals, each of the L3 and L4 stage changes is either severely delayed or never occurs, yet LC development before the early L3 stage or after the mid-L4 stage occurs with normal timing. We propose that there is a basal migration program utilized throughout LC migration that is modified by stage-specific regulators such as nhr-67.

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Development of the male reproductive system and sexual transformation in the nematode Caenorhabditis elegans

Cited by 121 publications

References 14 publications

Post-transcriptional regulation of sex determination in Caenorhabditis elegans: widespread expression of the sex-determining gene fem-1 in both sexes.

Post-transcriptional regulation of sex determination in Caenorhabditis elegans: widespread expression of the sex-determining gene fem-1 in both sexes.

Effects of radio-frequency fields on bacterial cell membranes and nematode temperature-sensitive mutants

The C. elegans tailless/Tlx homolog nhr-67 regulates a stage-specific program of linker cell migration in male gonadogenesis

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