Development of the intestinal microbiota in the piglet

Inoüe, Ryo; Tsukahara, Takamitsu; Nakanishi, Nobuo; Ushida, Kazunari

doi:10.2323/jgam.51.257

Cited by 72 publications

(81 citation statements)

References 36 publications

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“…As a consequence, they do not directly yield insight into the composition of a microbial community at any point, but they are a powerful means of detecting patterns of change in composition through space and time. Previous studies have shown that within-gel comparisons of electrophoretic fingerprints are highly reproducible and capable of resolving stable inter-individual differences in intestinal microbiota over time (Simpson et al, 1999(Simpson et al, , 2002Favier et al, 2002;Inoue et al, 2005b) Here, we have shown that the intestinal microbiota of piglets 430 days old is stable relative to that of younger piglets. A significant correlation was observed between housing environment and community structure.…”

Section: Introductionmentioning

confidence: 59%

“…Understanding the dynamics of microbial colonization requires a degree of control of these factors and the repeated sampling of individuals over the period from birth to complete immune development and/or independence from the mother. There are few studies that have used sampling intensities of weekly or better over this period (Favier et al, 2002;Inoue et al, 2005b) and only one that examined 410 individuals (Palmer et al, 2007). The general dynamics of microbial colonization remain very poorly known for any animal model.…”

Section: Introductionmentioning

confidence: 99%

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The immediate environment during postnatal development has long-term impact on gut community structure in pigs

2008

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The gastrointestinal tract of mammals contains a complex microbial community that influences numerous aspects of health and development. It is postulated that establishment of this community during early life has long-term consequences on the health status of adults. Potential influences on colonization are expected to include environmental microbes, diet and the developmental changes of the host. Denaturing gradient gel electrophoresis was used to follow the individual community dynamics of 24 piglets over the period of 3-36 days after birth. The community of piglets older than 31 days was inferred to show high stability relative to the first 28 days post birth. The stable day 36 community showed significant correlation between cohabiting piglets, but not between siblings. This cohabitation effect was not observable in 1-or 2-week-old piglets but was strongest at either 3 or 4 weeks post birth. The onset of this change after 2 weeks is predicted to be after the development of key induction elements of the immune system and before significant levels of piglet sIgA were observable (4 weeks). The outcome is altered community dynamics that result in significant similarity between the stable communities that develop in cohabiting pigs. We conclude that for a finite period in their development, the outcome of gut colonization in piglets is greatly influenced by the immediate environment. The implication is that mammals have a developmental window, in which the developing host-gut microbiota interaction will be simultaneously more amenable to engineering and more susceptible to disturbance.

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Section: Introductionmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 99%

The immediate environment during postnatal development has long-term impact on gut community structure in pigs

2008

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“…In the case of piglets, we also observed intensive colonization by bacteria, which is similar to the findings in rodents. However, the bacterial diversity still increased even at 50 days of age [6]. It has, therefore, been suggested that the mucosal immune system of weaning piglets does not develop well even at 50 days of age when piglets weigh in excess of 20 kg.…”

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confidence: 99%

Decreasing Traits of Fecal Immunoglobulin A in Neonatal and Weaning Piglets

Ushida

Kameue

Tsukahara

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. The concentration of fecal secretory immunoglobulin A (sIgA) in neonate and weaning piglets was measured daily from 1 day after birth to 50 days of age. The concentration of fecal sIgA started from the level of 10 4 µg/g wet feces 1 day after birth and then increased to a maximal value of up to 10 5 µg/g within a few days of birth. The values constantly declined to between 10 1 and 10 2 µg/ g for the next 10 days and were relatively constant until weaning. The level of sIgA in the feces remained very low until at least 50 days of age. The vulnerability of pre-or post-weaning piglets can be explained, at least in part, by this low level of sIgA in the intestine. KEY WORDS: feces, immunoglobulin A, piglet.

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“…13) One characteristic band in the exercise group was identified by a sequencing analysis according to the method described by Inoue et al, 14) the sequencing being performed by CEQ8000 (Beckman Coulter, Tokyo, Japan) at the Open Facility of Hokkaido University Sousei Hall. Five E. coli clones were randomly selected and sequenced in this study.…”

mentioning

confidence: 99%