Development of the infection strategy of the hemibiotrophic plant pathogen, Colletotrichum orbiculare, and plant immunity

Kubo, Yasuyuki; Harata, Ken; Kodama, Sayo; Fukada, Fumi

doi:10.1016/j.pmpp.2016.02.008

Cited by 14 publications

(9 citation statements)

References 55 publications

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“…The intracellular occurrence of this methoxylated form of sterigmatocystin in C. sublineolum points to the initiation of reprogramming of secondary metabolism during stage 1, characterised by the biosynthesis of toxins to be deployed during nutrient-limiting conditions as during stage 3 (6-demethylsterigmatocystin). This finding is consistent with previous studies that indicated that the biotrophic phase of Colletotrichum spp is marked by activation of a genomic program involved in secondary metabolism, directed also to toxin production 6,8,10,20 .…”

Section: Resultssupporting

confidence: 93%

“…and stationary (6–12 d.p.i.). These mathematically elucidated developmental time-periods of fungal growth correlate to previous studies, which reported that the biotrophic phase of Colletotrichum pathogens is short-lived, lasting about 72 h post-infection/inoculation, followed by the switch to necrotrophic lifestyle 6,10–12 . However, very little substantial knowledge exists with regards to the underlying biochemical and molecular mechanisms and differences in growth stages do not necessarily correspond to biotrophy or necrotrophy.…”

Section: Resultssupporting

confidence: 79%

“…The production and secretion of these phytotoxins (mostly non-host-specific) by Colletotrichum spp have been reported to be a marker for necrotrophic development. During this stage, Colletotrichum spp satisfy nutritional needs by actively killing the host tissue via phytotoxin production or generation of reactive oxygen species mechanisms 6,10,20,40,41 .…”

Section: Resultsmentioning

confidence: 99%

“…Extensive studies have been carried out using cytological, physiological, genomic and transcriptomic approaches to elucidate the molecular details that govern the lifestyles of hemibiotrophic Colletotrichum fungi, and have provided substantial knowledge of the underlying infection strategies 6–10 . Furthermore, considering high diversity and tractability to in vitro culture, Colletotrichum spp provide excellent models for studying the molecular mechanisms and biochemistry of infection structure differentiation and fungal-plant interactions 11,12 .…”

Section: Introductionmentioning

confidence: 99%

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Time-resolved decoding of metabolic signatures of in vitro growth of the hemibiotrophic pathogen Colletotrichum sublineolum

Tugizimana

Djami-Tchatchou

Fahrmann

et al. 2019

Sci Rep

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Metabolomics has emerged as a powerful approach to comprehensively interrogate cellular biochemistry. As such, we applied an untargeted liquid chromatography-mass spectrometry metabolomic strategy to elucidate metabolome changes in the anthracnose-causing hemibiotrophic sorghum pathogen, Colletotrichum sublineolum . An in vitro batch culture study model with different carbon sources, glucose, arabinose and rhamnose, were used to support fungal growth over a period of twelve days. Metabolites representing the intracellular and extracellular (secreted) metabolomes were extracted with methanol and subjected to LC-MS analyses. Chemometric modelling revealed a metabolic variation trajectory, comprising three distinct stages that metabolically describe the adaptation of the fungus to diminishing nutrients. Selected marker gene expression indicated stage one (0–3 d.p.i) as corresponding to the early logarithmic phase. Stage two can be interpreted as an intermediate transitionary stage with stage three corresponding to the stationary phase (9–12 d.p.i). Stage one was characterised by up-regulation of endo-metabolites such as ferricrocin, fatty acids and flavone-conjugates, while stage three was characterised by the secretion of phytotoxins, including colletotrichin and colletotric acid. Ultimately, results from our in vitro model reveal previously unknown insights into the dynamic aspects of metabolome reprogramming in the growth phases of Colletotrichum spp as determined by nutrients obtainable from plant cell walls.

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Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 79%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Time-resolved decoding of metabolic signatures of in vitro growth of the hemibiotrophic pathogen Colletotrichum sublineolum

Tugizimana

Djami-Tchatchou

Fahrmann

et al. 2019

Sci Rep

View full text Add to dashboard Cite

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“…Adhesion to the plant surface is the first step in initiation of the infection process in many plant pathogenic fungi [ 49 ]. Following adhesion, physical signals such as those involving tissue hardness and hydrophobicity or chemical signals (cutin monomers and leaf waxes) induce germination and appressorial formation in several plant pathogenic fungi [ 50 ].…”

Section: Virulence Factorsmentioning

confidence: 99%

Colletotrichum higginsianum as a Model for Understanding Host–Pathogen Interactions: A Review

Yan

Yuan

Tang

et al. 2018

IJMS

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Colletotrichum higginsianum is a hemibiotrophic ascomycetous fungus that causes economically important anthracnose diseases on numerous monocot and dicot crops worldwide. As a model pathosystem, the Colletotrichum–Arabidopsis interaction has the significant advantage that both organisms can be manipulated genetically. The goal of this review is to provide an overview of the system and to point out recent significant studies that update our understanding of the pathogenesis of C. higginsianum and resistance mechanisms of Arabidopsis against this hemibiotrophic fungus. The genome sequence of C. higginsianum has provided insights into how genome structure and pathogen genetic variability has been shaped by transposable elements, and allows systematic approaches to longstanding areas of investigation, including infection structure differentiation and fungal–plant interactions. The Arabidopsis-Colletotrichum pathosystem provides an integrated system, with extensive information on the host plant and availability of genomes for both partners, to illustrate many of the important concepts governing fungal–plant interactions, and to serve as an excellent starting point for broad perspectives into issues in plant pathology.

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Epidemiology and Management of Fungal Diseases in Dry Environments

Al-Sadi

2016

Innovations in Dryland Agriculture

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Development of the infection strategy of the hemibiotrophic plant pathogen, Colletotrichum orbiculare, and plant immunity

Cited by 14 publications

References 55 publications

Time-resolved decoding of metabolic signatures of in vitro growth of the hemibiotrophic pathogen Colletotrichum sublineolum

Time-resolved decoding of metabolic signatures of in vitro growth of the hemibiotrophic pathogen Colletotrichum sublineolum

Colletotrichum higginsianum as a Model for Understanding Host–Pathogen Interactions: A Review

Epidemiology and Management of Fungal Diseases in Dry Environments

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