Development of the genital microflora in stallions used for artificial insemination throughout the breeding season

Pasing, Stephanie; Aurich, Christine; Lewinski, Mareike von; Wulf, Manuela; Krüger, Monika; Aurich, Jörg

doi:10.1016/j.anireprosci.2013.03.009

Cited by 27 publications

(40 citation statements)

References 16 publications

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“…In contrast to the donkey jack, a number of studies have been carried out that investigated the aerobic bacterial flora in the reproductive tract of stallions (Madsen and Christensen, 1995;Malmgren et al, 1998;Pasing et al, 2013;Scherbarth et al, 1994;Vaissaire et al, 1987). The majority focused on the frequency of potential venereal pathogens in semen, in the urethra or from an artificial vagina.…”

Section: Discussionmentioning

confidence: 99%

“…The majority focused on the frequency of potential venereal pathogens in semen, in the urethra or from an artificial vagina. Pasing et al (2013) undertook an extensive study of the aerobic and facultative anaerobic bacteria that could be cultured from breeding stallions in active use throughout a breeding season. They demonstrated an increase in total microbial growth as the season progressed.…”

Section: Discussionmentioning

confidence: 99%

“…Similar to what was found in the current study, multiple bacterial species were cultured from mucosal sites, especially from the surface of the penis/prepuce and from the urethral fossa. Pasing et al (2013) cultured a total of 33 bacterial species from the penile/preputial surface. Akin to the donkey findings, the same authors did not identify any specific pathogens and only an occasional organism (Escherichia coli, Aspergillus fumigatus, Mucor spp.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Bacterial and fungal microflora on the external genitalia of male donkeys (Equus asinus)

Carleton

Donahue

Marteniuk

et al. 2015

Animal Reproduction Science

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Bacterial and fungal microflora on the external genitalia of male donkeys (Equus asinus)

Carleton

Donahue

Marteniuk

et al. 2015

Animal Reproduction Science

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“…La mayoría de los eyaculados recolectados de animales sanos se encuentran contaminados con microorganismos (Kristula y Smith, 2004; Morrell y Wallgren, 2011), los cuales podría esperarse que provengan de la mucosa del prepucio y la uretra (Pasing et al, 2013). En el semen equino se ha reportado una moderada incidencia de los géneros Corynebacterium y Staphylococcus (coagulasa-negativos) y de la clase Actinobacteria.…”

Section: Discussionunclassified

“…En el semen equino se ha reportado una moderada incidencia de los géneros Corynebacterium y Staphylococcus (coagulasa-negativos) y de la clase Actinobacteria. De igual forma, se reporta una baja incidencia de microorganismos de la familia Enterobacteriacae, de los géneros Micrococcus, Bacillus, Streptomyces y Streptococcus (alfa-hemolíticos) y de las bacterias Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa y Streptococcus equi, entre otras (Madsen y Christensen, 1995; Dirscherl, 2011; Pasing et al, 2013). Entre los mohos, se ha reportado el Aspergillus sp y entre las levaduras Candida albicans (Vaid et al, 2012).…”

Section: Discussionunclassified

Proliferación Microbiana y Calidad Posdescongelación de Semen Equino Criopreservado en Presencia de Antibióticos

Betancur

Mazo

Cortés

2016

Rev. investig. vet. Perú

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RESUMENLos antibióticos son utilizados en la criopreservación de semen equino con el fin de controlar la proliferación de microorganismos; sin embargo, estos pueden tener efectos negativos sobre los espermatozoides. La presente investigación tuvo como objetivo evaluar el efecto de dos preparaciones antibióticas sobre el desarrollo microbiano y la calidad de semen equino criopreservado. El semen de 10 caballos criollos colombianos fue criopreservado por congelación convencional, con dos preparaciones antibióticas: P1 (penicilina 10000 UI/ml y estreptomicina 10 mg/ml) y P2 (penicilina 10000 UI/ml, estreptomicina 10 mg/ml y anfotericina B 25 µg/ml) y un grupo control sin antibióticos. Posterior a la descongelación, se realizó la evaluación computarizada de la movilidad espermática, la prueba hipoosmótica (HOS) y el conteo de bacterias y hongos en el semen. El análisis estadístico se realizó mediante modelos lineales generalizados (GLM) y la comparación de medias por Tukey. Se encontró diferencia significativa (p<0.05) para movilidad total (MT%) entre P1 (32.2 ± 17.6) y P2 (34.4 ± 19.1) con el grupo control (39.7 ± 16.5) y para movilidad progresiva (MP%) entre P1 (15.5 ± 14.8) con P2 (17.9 ± 16.5) y el control (19.6 ± 14.1). Para la proliferación microbiana se encontró diferencia significativa (p<0.05) para bacterias (UFC/ml) entre P1 (167.7 ± 237.1) y P2 (240.1 ± 321.0) con el grupo control (464.4 ± 448.5). El uso de preparaciones antibióticas con penicilina, estreptomicina y anfotericina B en la criopreservación de semen equino reduce la proliferación de bacterias, pero produce una disminución en la movilidad espermática posdescongelación.

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Bacterial diversity in semen from stallions in three European countries evaluated by 16S sequencing

Malaluang,

Niazi,

Guo

et al. 2024

Vet Res Commun

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The microbiome plays a significant role in shaping the health and functioning of the systems it inhabits. The seminal microbiome of stallions has implications for the health of the reproductive tract, sperm quality during preservation and antibiotic use in semen extenders. Diverse bacteria are present on the external genital tract and a mix of commensal microorganisms populates various parts of the reproductive tract, influencing the seminal bacterial content. Other sources of bacteria include the environment, semen collection equipment, and personnel. The bacterial load can adversely affect sperm quality and fertility, particularly in artificial insemination, where semen is extended and stored before use. Antibiotics are frequently used to inhibit bacterial growth, but their effectiveness varies depending on the bacterial strains present. The aim of this study was to assess the bacterial diversity in semen from 37 healthy stallions across three European nations (Germany, Portugal, and Sweden) using 16S sequencing. Semen samples were collected from individual stallions at three AI centers; DNA extraction, sequencing, and bioinformatic analysis were performed. Differences in bacterial diversity among the stallions were seen; although bacterial phyla were shared across the regions, differences were observed at the genus level. Climate, husbandry practices, and individual variability likely contribute to these differences. These findings underscore the importance of tailoring antibiotic strategies for semen preservation based on regional bacterial profiles. The study presents a comprehensive approach to understanding the intricacies of the stallion seminal microbiome and its potential implications for reproductive technologies and animal health.

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Development of the genital microflora in stallions used for artificial insemination throughout the breeding season

Cited by 27 publications

References 16 publications

Bacterial and fungal microflora on the external genitalia of male donkeys (Equus asinus)

Bacterial and fungal microflora on the external genitalia of male donkeys (Equus asinus)

Proliferación Microbiana y Calidad Posdescongelación de Semen Equino Criopreservado en Presencia de Antibióticos

Bacterial diversity in semen from stallions in three European countries evaluated by 16S sequencing

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