Development of taro (Colocasia esculenta (L.) Schott) hybrids overcoming its asynchrony in flowering using cryostored pollen

Mukherjee, Archana; George, James; Pillai, R. N.; Chakrabarti, S. K.; Naskar, S. K.; Patro, Rashmishree; Nayak, Shubhransu; Lebot, Vincent

doi:10.1007/s10681-016-1745-8

Cited by 6 publications

(7 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is applicable to a range of plant tissues such as pollen, seed, shoot tips, somatic and zygotic embryos, cell suspensions, dormant buds, etc. [62,63]. High-quality pollen, dehydrated to optimal moisture content and stored at liquid nitrogen temperatures, can be stored for over 10 years [43,59].…”

Section: Storage Methodsmentioning

confidence: 99%

Floral Biology and Pollination Efficiency in Yam (Dioscorea spp.)

et al. 2020

View full text Add to dashboard Cite

Yam (Dioscorea spp.) is a monocotyledonous herbaceous vine cultivated for its starchy underground or aerial tubers in the tropics and subtropics. It is an allogamous and polyploid species that reproduces by both sexual and asexual mechanisms. However, many of the landrace cultivars, including most of the popular varieties, reproduce exclusively by vegetative propagation (planting the tubers). These varieties are either sterile or produce sparse and irregular flowering with high flower abortion rate, low fruit and seed set. Production of crossbreed seeds for genetic improvement and for maintaining genetic diversity in yams is, therefore, mainly achieved through natural or managed pollination. Flowering in yam is mostly dioecious and, in some instances, monoecious. Flowering asynchrony, sticky nature of the pollen grains, and cross incompatibility are among the challenges in making genetic progress in yam breeding. There are many limitations in basic and applied knowledge of yam flower biology and pollination. This paper, therefore, reviews the flowering biology, pollination, and methods of improving pollination efficiency in yam breeding programs.

show abstract

Section: Storage Methodsmentioning

confidence: 99%

Floral Biology and Pollination Efficiency in Yam (Dioscorea spp.)

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In India, breeding was also hindered due to asynchrony in flowering and an attempt was made to cryostore pollen at different time intervals ranging from 1 week to 2 months and to use the cryostored pollen for hybridization with introduced genotypes. Fruit setting was observed within a week of crossing introduced genotypes with cryopreserved pollens (Mukherjee et al 2016). Introduced genotypes were also crossed in Bhubaneswar (Orissa) and 31 crosses recombining TANSAO cultivars and Samoa hybrids were successful.…”

Section: Resultsmentioning

confidence: 99%

“…The somewhat limited number of successful controlled crosses (Table 4) is impairing the creation of diversity needed for further selection but asynchrony, pollen viability and the receptiveness of female flowers are severely impacting the success rates of these crosses. Protocols for the cryopreservation of pollen, either freeze dried or cryostored in liquid nitrogen have been developed (Mukherjee et al 2016) but more work is needed, especially with freeze dried pollen in order to ease and speed up the control crosses.…”

Section: Discussionmentioning

confidence: 99%

Adapting clonally propagated crops to climatic changes: a global approach for taro (Colocasia esculenta (L.) Schott)

Lebot

Tuia²,

Ivančič

et al. 2017

Genet Resour Crop Evol

View full text Add to dashboard Cite

Clonally propagated crop species are less adaptable to environmental changes than those propagating sexually. DNA studies have shown that in all countries where taro (Colocasia esculenta (L.) Schott) has been introduced clonally its genetic base is narrow. As genetic variation is the most important source of adaptive potential, it appears interesting to attempt to increase genetic and phenotypic diversity to strengthen smallholders' capacity to adapt to climatic changes. A global experiment, involving 14 countries from America, Africa, Asia and the Pacific was conducted to test this approach. Every country received a set of 50 indexed genotypes in vitro assembling significant genetic diversity. After onstation agronomic evaluation trials, the best genotypes were distributed to farmers for participatory on-farm evaluation. Results indicated that hybrids tolerant to taro leaf blight (TLB, Phytophthora colocasiae Raciborski), developed by Hawaii, Papua New Guinea and Samoa breeding programmes outperformed local

show abstract

“…The first application was mentioned on embryogenic callus by Shimonishi et al [77] using a slow freezing method. Later, other cryopreservation techniques were applied, including vitrification [78,79], droplet vitrification [80,81], and encapsulation-dehydration [76,82] on various explants, such as shoot tips [76,78,79,81,82], apical meristems [80], pollen [83], and axillary buds [78]. C. esculenta is a species characterized by its asynchronous flowering.…”

Section: Aracaceaementioning

confidence: 99%

“…C. esculenta is a species characterized by its asynchronous flowering. To overcome this issue, an experiment was conducted to preserve the pollen in liquid nitrogen and then use the cryostored pollen for hybridization of taro [83]. The pollen was maintained in LN for different time intervals, ranging from 1 week to 2 months.…”

Section: Aracaceaementioning

confidence: 99%

The Cryopreservation of Medicinal and Ornamental Geophytes: Application and Challenges

Merzougui

Benelli̇

Boullani

et al. 2023

Plants

View full text Add to dashboard Cite

Nowadays, plant genetic resources are often at risk of loss and destruction. Geophytes are herbaceous or perennial species that are annually renewed by bulbs, rhizomes, tuberous roots, or tubers. They are often subject to overexploitation, which, combined with other biotic and abiotic stresses, can make these plants more vulnerable to a decline in their diffusion. As a result, multiple endeavors have been undertaken to establish better conservation strategies. Plant cryopreservation at ultra-low temperatures in liquid nitrogen (−196 °C) has proven to be an effective, long-term, low-cost, and suitable conservation method for many plant species. Over the last two decades, major advances in cryobiology studies have enabled successful explants of multiple genera and types, including pollen, shoot tips, dormant buds, and zygotic and somatic embryos. This review provides an update on recent advances and developments in cryopreservation and its application to medicinal and ornamental geophytes. In addition, the review includes a brief summary of factors limiting the success of bulbous germplasm conservation. The critical analysis underpinning this review will benefit biologists and cryobiologists in their further studies on the optimization of geophyte cryopreservation protocols and will support a more complete and wider application of knowledge in this area.

show abstract

Development of taro (Colocasia esculenta (L.) Schott) hybrids overcoming its asynchrony in flowering using cryostored pollen

Cited by 6 publications

References 7 publications

Floral Biology and Pollination Efficiency in Yam (Dioscorea spp.)

Floral Biology and Pollination Efficiency in Yam (Dioscorea spp.)

Adapting clonally propagated crops to climatic changes: a global approach for taro (Colocasia esculenta (L.) Schott)

The Cryopreservation of Medicinal and Ornamental Geophytes: Application and Challenges

Contact Info

Product

Resources

About