Development of Surface Plasmon Resonance (SPR) Based Immuno-Sensing System for Detection of Fungal Teliospores of Karnal Bunt (Tilletia indica), a Quarantined Disease of Wheat

Singh, Saurabh

doi:10.4172/2155-6210.1000125

Cited by 6 publications

(3 citation statements)

References 26 publications

(29 reference statements)

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“…Primary and secondary sporidia or hyphae as compatible mating types must fuse to produce dikaryon that increases the chances of variation due to heterozygosity, which plays an important role in developing new variants [ 15 ]. The fungus infects the ovaries in the developing heads of wheat and transforms the seed to a dark colored powdery mass consisting of millions of teliospores [ 16 , 17 ]. The most effective carrier of pathogens for inter-regional and long-range spore dispersal is infected seeds [ 18 ].…”

Section: Pathogen Biologymentioning

confidence: 99%

Centenary of Soil and Air Borne Wheat Karnal Bunt Disease Research: A Review

Iquebal

Mishra

Maurya

et al. 2021

Biology

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Karnal bunt (KB) of wheat (Triticum aestivum L.), known as partial bunt has its origin in Karnal, India and is caused by Tilletia indica (Ti). Its incidence had grown drastically since late 1960s from northwestern India to northern India in early 1970s. It is a seed, air and soil borne pathogen mainly affecting common wheat, durum wheat, triticale and other related species. The seeds become inedible, inviable and infertile with the precedence of trimethylamine secreted by teliospores in the infected seeds. Initially the causal pathogen was named Tilletia indica but was later renamed Neovossia indica. The black powdered smelly spores remain viable for years in soil, wheat straw and farmyard manure as primary sources of inoculum. The losses reported were as high as 40% in India and also the cumulative reduction of national farm income in USA was USD 5.3 billion due to KB. The present review utilizes information from literature of the past 100 years, since 1909, to provide a comprehensive and updated understanding of KB, its causal pathogen, biology, epidemiology, pathogenesis, etc. Next generation sequencing (NGS) is gaining popularity in revolutionizing KB genomics for understanding and improving agronomic traits like yield, disease tolerance and disease resistance. Genetic resistance is the best way to manage KB, which may be achieved through detection of genes/quantitative trait loci (QTLs). The genome-wide association studies can be applied to reveal the association mapping panel for understanding and obtaining the KB resistance locus on the wheat genome, which can be crossed with elite wheat cultivars globally for a diverse wheat breeding program. The review discusses the current NGS-based genomic studies, assembly, annotations, resistant QTLs, GWAS, technology landscape of diagnostics and management of KB. The compiled exhaustive information can be beneficial to the wheat breeders for better understanding of incidence of disease in endeavor of quality production of the crop.

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Section: Pathogen Biologymentioning

confidence: 99%

Centenary of Soil and Air Borne Wheat Karnal Bunt Disease Research: A Review

Iquebal

Mishra

Maurya

et al. 2021

Biology

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“…A1 Quarantine pests such as Karnal bunt (Tilletia indica Mitra) of wheat has been detected using bio-sensor technique and shown adequate to perform a rapid, easy, and reliable analysis. This technique of detection in order to identify the teliospores of contaminating fungus in wheat lots, which could be used by the seed certification laboratory and plant quarantine department (S. Singh et al, 2012). Furthermore, the use of sensor for the detection of various plant pathogens include the use of red green blue (RGB) sensor for detection of Cercospora leaf spot disease (Cercospora beticola Sacc) in sugar plant (Neumann et al, 2014), the use of Spectral sensors against brown spot disease (Puccinia hordei Otth), and powdery mildew (Blumeria graminis DC Speer)) in barley (Kuska et al, 2015;Wahabzada et al, 2015).…”

Section: Plant Quarantine In the Industry Era 40mentioning

confidence: 99%

New Paradigm on Plant Quarantine System for Protection of Biological Diversity in Indonesia

Tasrif

Taufik

Nazaruddin

2021

JPTI

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Plant quarantine system becomes an important pillar in the protection of biological diversities from the threat of plant pests and diseases. The implementation of plant quarantine system currently covers prevention of spread of quarantine pests, food safety, food quality, genetic resources and bio-agents, as well as invasive alien species and genetically modified organism. During 2014 to 2018, a total of 232 frequency intercepted of quarantine pests of viruses, bacteria, fungi, nematodes, insects, and weeds have been detected. These pests associated with plant materials from various countries in Asia, Europe, the United States, Australia and Africa that may potentially threaten biological diversities when dispersed within Indonesian territory. Implementation of risk analysis and appropriate level of protection consideration can be clustered in to pre-border, at-border, and post-border activities in order to mitigate the risk of quarantine pests and biosafety monitoring into Indonesian territory. Utilizing advances in pest detection technology in the industrial era 4.0 could provide benefits in the agricultural sectors. Various detection technologies using drones and bio-sensors have contributed in the field of plant protection, especially as pest detection and monitoring tools in the field. Furthermore, the establishment of proficiency certification agency for plant quarantine systems may contribute efficient and effective operations in the near future.

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“…In the past few decades, the surface plasmon resonance (SPR) technique has been successfully explored for the detection of biological anlaytes for environmental monitoring, drug discovery, food science and biomedical applications [8][9][10][11][12]. Amongst the label-free detection techniques, SPR based sensors have emerged as a powerful optical tool for real time measurements of biomolecular events with high sensitivity [9,13,14].…”

Section: Introductionmentioning

confidence: 99%

Smaller to larger biomolecule detection using a lab-built surface plasmon resonance based instrument

et al. 2016

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We have developed a low-cost surface plasmon resonance (SPR) instrument based on the Kretschmann configuration for biosensing applications. The fabricated instrument is capable of operating in both angular and intensity interrogation schemes. The proposed sensor has proved enormously versatile by detecting a range of analytes with low to high molecular weights. The refractive index based sensor has been used for detecting the variation in the concentration of the aqueous solution of glucose and glycerine. Real time immobilization of protein molecules, bovine serum albumin on a gold (Au) film surface, has also been detected using the SPR imaging technique. Alkanethiol functionalization of the Au surface was performed, and bovine serum albumin was immobilized onto the carboxyl functionalized surface using amine reactive cross linker chemistry. In future, the present approach can also be utilized for the selective detection of a wide range of target biomolecules with the help of specific capture probes, as well as for monitoring protein–drug interactions.

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Development of Surface Plasmon Resonance (SPR) Based Immuno-Sensing System for Detection of Fungal Teliospores of Karnal Bunt (Tilletia indica), a Quarantined Disease of Wheat

Cited by 6 publications

References 26 publications

Centenary of Soil and Air Borne Wheat Karnal Bunt Disease Research: A Review

Centenary of Soil and Air Borne Wheat Karnal Bunt Disease Research: A Review

New Paradigm on Plant Quarantine System for Protection of Biological Diversity in Indonesia

Smaller to larger biomolecule detection using a lab-built surface plasmon resonance based instrument

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