Development of stabilized Paclitaxel nanocrystals: In-vitro and in-vivo efficacy studies

Investigating the role of Pluronic-g-Cationic polyelectrolyte as functional stabilizer for nanocrystals: Impact on Paclitaxel oral bioavailability and tumor growth

“…On the other hand almost similar z-average of nanocrystals was observed at stabilizer concentration 0.1% w/v (186.12±12.31nm) and 0.2% w/v (180.78±9.2nm) immediately after processing and this was in agreement to our previous report [21] where we found that once the stabilizer concentration is sufficient to cover the generated surface, further increase in stabilizer concentration did not affect the size reduction and PDI. Moreover, there was no significant change in particle size in these two formulations 1 hour after of formulation development [47].…”

“…The results are shown in Fig.2 and as reported previously the mean particle size and PDI both decreased while the pressure increased from 1000 bars to 1800 bars [47]. The desired particle size range 150-225 nm was obtained at 1800 bars (10 cycles) and no further reduction in particle size was observed on increasing the number of cycles.…”

“…Here we are investigating the potential of Pluronic (PF127) grafted chitosan (Pl-g-CH) as a functional stabilizer during development of PTX nanocrystals for oral delivery. In our previous work we reported that that Sodium poly styrene sulfonate (PSS) stabilized NCs can significantly improve the PTX bioavailability compared to coarse drug suspension .Briefly [21] in our previous experiments we had investigated the nanocrystals stabilizing potential of different type of molecules like non ionic surfactant (Tween 80), natural polyelectrolytes (sodium alginate and glycol chitosan) and synthetic polyelectrolyte (Sodium poly styrene sulfonate). We had observed that PSS stabilized nanocrystals were much more stable as compared to both non ionic surfactants as well as natural polyelectrolytes stabilized nanocrystals.…”

Evaluation of <i>In vitro</i> Antioxidant and <i>In vivo</i> Antihyperlipidemic Activities of Methanol Extract of Aerial Part of <i>Crassocephalum crepidioides</i> (Asteraceae) Benth S Moore

“…There is evidence that macrophages in atherosclerotic lesions express myeloperoxidase that yields a unique pattern of protein oxidation products. Myeloperoxidase is also pinpointed as a pathway that promotes LDL oxidation [7]. Natural products with antioxidant potential have been proposed as effective in the treatment of metabolic syndrome like insulin resistance, diabetes, obesity, altered lipid profile, and hypertension [8][9][10] The genus Crassocephalum is in the family Asteraceae (Compositae) in the major group Angiosperms (Flowering plants).…”

Evaluation of anti-cancer properties of pegylated ethosomal paclitaxel on human melanoma cell line SKMEL- 3

“…To increase solubility, the drug is mixed with Cremophor EL and alcohol under the trade names, Taxol® or Paxene®. Cremophor EL is a solvent for biological and pharmacological substances but has various side effects, such as hypersensitivity, nephrotoxicity, neurotoxicity and hyperlipidemia, as well as alters the pharmacokinetics of Paclitaxel® [4]. Therefore, the use of nanotechnology-based devices as Paclitaxel® carriers is recommended to enhance therapeutic efficacy and decrease the side effects of the drug [5].…”