Development of Specific Electrochemical Biosensor Based on Chitosan Modified Screen Printed Carbon Electrode for the Monitoring of Captan Fungicide

Wongkaew, Porntip

doi:10.21660/2018.43.3547

Cited by 1 publication

(2 citation statements)

References 13 publications

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“…AFM requires mostly planar surfaces, however it also allowed to study the presence of laccase on highly‐oriented pyrolytic graphite (HOPG) after different adsorption times (20 s, 30 min and 24 h) . We quote a latter case where individual molecules were not distinguished: AFM was used to show changes of surface morphology at the different steps of the creation of an electrode made of screen printed carbon covered by chitosan and finally by glutathione‐S‐transferase …”

Section: Electrode Imaging To Confirm the Presence Of Enzymesmentioning

confidence: 99%

“…[57] We quote a latter case where individual molecules were not distinguished: AFM was used to show changes of surface morphology at the different steps of the creation of an electrode made of screen printed 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 carbon covered by chitosan and finally by glutathione-Stransferase. [58]…”

Section: Afmmentioning

confidence: 99%

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Micro‐ and Nanoscopic Imaging of Enzymatic Electrodes: A Review

2019

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Redox enzymes, which catalyze electron transfer reactions in living organisms, can be used as selective and sensitive bioreceptors in biosensors, or as efficient catalysts in biofuel cells. In these bioelectrochemical devices, the enzymes are immobilized at a conductive surface, the electrode, with which they must be able to exchange electrons. Different physicochemical methods have been coupled to electrochemistry to characterize the enzyme‐modified electrochemical interface. In this Review, we summarize most efforts performed to investigate the enzymatic electrodes at the micro‐ and even nanoscale, thanks to microscopy techniques. Contrary to electrochemistry, which gives only a global information about all processes occurring at the electrode surface, microscopy offers a spatial resolution. Several techniques have been implemented; mostly scanning probe microscopies like atomic force microscopy, scanning tunneling microscopy, and scanning electrochemical microscopy, but also scanning electron microscopy and fluorescence microscopy. These studies demonstrate that various information can be obtained thanks to microscopy at different scales. Electrode imaging has been performed to confirm the presence of enzymes, to quantify and localize the biomolecules, but also to evaluate the morphology of immobilized enzymes, their possible conformation changes upon turnover, and their orientation at the electrode surface. Local redox activity has also been imaged and kinetics has been resolved.

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Section: Electrode Imaging To Confirm the Presence Of Enzymesmentioning

confidence: 99%

Section: Afmmentioning

confidence: 99%