Development of Solid Lipid Nanoparticles of Gac (Momordica cocochinensis Spreng) Oil by Nano-Emulsion Technique

Abstract

Solid lipid nanoparticles have emerged as a next-generation drug delivery system with potential applications in the pharmaceutical field, cosmetics, research, clinical medicine and other allied sciences. In the present work, gac oil-loaded solid lipid nanoparticles were successfully prepared by a nano emulsion technique employing high-speed homogenizer. In this study, solid lipid nanoparticle were successfully prepared by using Emulgade SE-PF TM lipid, high-speed homogenization with the support of surfactant. The suspensions contained 5 % active agents (gac oil w/w) were dispersed in the presence of surfactant (Tween-80:Span-80 ratio of 72:28 w/w) at the content of 5 % by using the IKA homogenizer at the speed of 10000 rpm for 60 min (30 min hot homogenization and 30 min cold homogenization). The obtained mixture had the median size of 200 nm (by laser diffractometric spectrometry, LSD). Entrapment efficiency was about 90.8 %.

Plant oils: From chemical composition to encapsulated form use

“…However, further increase in speed leads to increase the particle size due to the fact that the oil particles are more likely to collide and aggregate with others. Concerning hot homogenization time, short time was found not enough for active ingredient encapsulation while more homogenization time result in small particles which in turn lead to reduce the volume containing active ingredients (Mai et al, 2018).…”

“…Several factors such as the kind of lipid, surfactant and the operating process conditions could influence the physicochemical properties of the particles obtained by high-pressure homogenization. In this context, Mai et al (2018) investigated the influence of homogenization conditions on gac fruit EO-loaded SLN prepared by hot and cold homogenization. Results…”

“…Lipids like tristearin (Salminen et al, 2017), n-hexadecyl palmitate (Lacatusu et al, 2015), myritol ® 318 (caprylic/capric triglyceride) (Lacatusu et al, 2015), compritol ® 888 ATO (Wang, 2012), glyceryl monolinoleate (Shi et al, 2013;Tian et al, 2018), oleoyl macrogolglycerides (Shi et al, 2013), lipoid ® S100 (Sidding et al, 2013), cetyl palmitate (Svetlichny et al, 2015) and glycerol monostearate (Shi et al, 2013) are usually used to structure either SLN or NLC. Soybean lecithin (Salminen et al, 2017;Wang, 2012), tween 80 (Shi et al, 2013;Sidding et al, 2013;Svetlichny et al, 2015;Wang, 2012), tween 20 (Badea et al, 2015;Lacatusu et al, 2015), span 80 (Mai et al, 2018;Tian et al, 2018) poloxamer (Badea et al, 2015) and sodium taurodeoxycholate hydrate (Salminen et al, 2017) are example of used emulsifiers.…”

Antioxidant and Anti-Elastase Activity of Ethanol Extract of Tomato (Solanum lycopersicum L.)

“…The lycopene has high antioxidant activity, antiaging ability, skin tanning, skincare, skin protection activity, and inhibition of the stromal fibroblasts' migration. Tomatoes also contained flavonoids with intense antioxidant activity and elastase inhibition (Kashif et al 2017;Mai et al 2018;Simitzis 2018). The antioxidant activity could be investigated using 2,2'-azino-bis 3ethylbenzothiazoline-6-sulphonic acid (ABTS) assay.…”

Evaluation of Conditions Affecting Properties of Gac (Momordica Cocochinensis Spreng) Oil-Loaded Solid Lipid Nanoparticles (SLNs) Synthesized Using High-Speed Homogenization Process

“…Among many types of nano-carriers, solid lipid nanoparticles (SLNs) are proven ideal for the encapsulation of highly lipophilic bioactive compounds [18], drugs or antioxidants [19][20][21]. SLNs comprise spherical lipid particles with a nanometer size range of 50-1000 nm [22,23] and are capable of facilitating the dissolution of poorly water-soluble compounds and controlling the release effectively [24].…”