Development of single chain variable fragment (scFv) antibodies against Xylella fastidiosa subsp. pauca by phage display

Yuan, Qing; Jordan, R.; Brlansky, R. H.; Istomina, Olga Borisovna; Hartung, John

doi:10.1016/j.mimet.2015.07.020

Cited by 14 publications

(6 citation statements)

References 36 publications

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“…The phages were precipitated from the supernatant with 5× PEG/NaCl (20% PEG/2.5 M NaCl) and resuspended in 1 ml of PBS and then precipitated again to remove the bacterial cells. The phages were then resuspended in 100 to 200 μl of PBS+1% BSA [16] , [17] , [18] .…”

Section: Methodsmentioning

confidence: 99%

A Single Domain–Based Anti-Her2 Antibody Has Potent Antitumor Activities

Chen

Lin

et al. 2018

Translational Oncology

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Human epidermal growth factor receptor 2 (HER2) is overexpressed in approximately 20% to 30% of breast cancers and various other types of cancers, which plays a vital role in the cancer progression. Monoclonal antibodies targeting Her2 are now used in the clinic to treat Her2 overexpression cancer patients. However, relapse or resistance is frequent with the current therapies. To generate a new treatment avenue against Her2, we immunized and selected a specific anti-Her2 single domain antibody C3 for further studies. The C3-Fc antibody drove antibody-dependent cell-mediated cytotoxicity against Her2-positive tumor cells in vitro and resulted in potent antitumor growth in vivo. These data suggest that the C3-Fc antibody may provide an alternative avenue for Her2-positive cancer therapy.

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Section: Methodsmentioning

confidence: 99%

A Single Domain–Based Anti-Her2 Antibody Has Potent Antitumor Activities

Chen

Lin

et al. 2018

Translational Oncology

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“…Anyway, most of the antibody-based detection assay are effective only at the species level, while at our knowledge a single report is available describing the development of single chain variable fragment (scFv) antibodies specific for X. fastidiosa subsp. pauca (Yuan et al, 2015). Another detection method that since the nineties has become more and more popular relies on PCR amplification of bacterial DNA.…”

Section: Hosts and Diseasesmentioning

confidence: 99%

Xylella fastidiosa: Host Range and Advance in Molecular Identification Techniques

Baldi

Porta

2017

Front. Plant Sci.

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In the never ending struggle against plant pathogenic bacteria, a major goal is the early identification and classification of infecting microorganisms. Xylella fastidiosa, a Gram-negative bacterium belonging to the family Xanthmonadaceae, is no exception as this pathogen showed a broad range of vectors and host plants, many of which may carry the pathogen for a long time without showing any symptom. Till the last years, most of the diseases caused by X. fastidiosa have been reported from North and South America, but recently a widespread infection of olive quick decline syndrome caused by this fastidious pathogen appeared in Apulia (south-eastern Italy), and several cases of X. fastidiosa infection have been reported in other European Countries. At least five different subspecies of X. fastidiosa have been reported and classified: fastidiosa, multiplex, pauca, sandyi, and tashke. A sixth subspecies (morus) has been recently proposed. Therefore, it is vital to develop fast and reliable methods that allow the pathogen detection during the very early stages of infection, in order to prevent further spreading of this dangerous bacterium. To this purpose, the classical immunological methods such as ELISA and immunofluorescence are not always sensitive enough. However, PCR-based methods exploiting specific primers for the amplification of target regions of genomic DNA have been developed and are becoming a powerful tool for the detection and identification of many species of bacteria. The aim of this review is to illustrate the application of the most commonly used PCR approaches to X. fastidiosa study, ranging from classical PCR, to several PCR-based detection methods: random amplified polymorphic DNA (RAPD), quantitative real-time PCR (qRT-PCR), nested-PCR (N-PCR), immunocapture PCR (IC-PCR), short sequence repeats (SSRs, also called VNTR), single nucleotide polymorphisms (SNPs) and multilocus sequence typing (MLST). Amplification and sequence analysis of specific targets is also mentioned. The fast progresses achieved during the last years in the DNA-based classification of this pathogen are described and discussed and specific primers designed for the different methods are listed, in order to provide a concise and useful tool to all the researchers working in the field.

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“…The 5-year $9 million NuPsyllid project formulated by CRDF and funded by USDA NIFA concluded in 2017. The very ambitious overall goal of the project, to develop a modified psyllid that would not transmit CLas and could be deployed in the field to displace the natural ACP population, was not achieved but the project did contribute significant advances to the fundamental knowledge of ACP-CLas biology (Soderlund et al, 2014;Cicero et al, 2015;Ding et al, 2015Ding et al, , 2016Ding et al, , 2017Yuan et al, 2015Yuan et al, , 2016Brown et al, 2016;Stover et al, 2016;Liu et al, 2017).…”

Section: Notable Research Outcomesmentioning

confidence: 99%

A Review of the Citrus Greening Research and Development Efforts Supported by the Citrus Research and Development Foundation

Earth¹

2018

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President Lincoln, as a private, nongovernmental institution to advise the nation on issues related to science and technology. Members are elected by their peers for outstanding contributions to research. Dr. Marcia McNutt is president. The National Academy of Engineering was established in 1964 under the charter of the National Academy of Sciences to bring the practices of engineering to advising the nation. Members are elected by their peers for extraordinary contributions to engineering.

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Development of single chain variable fragment (scFv) antibodies against Xylella fastidiosa subsp. pauca by phage display

Cited by 14 publications

References 36 publications

A Single Domain–Based Anti-Her2 Antibody Has Potent Antitumor Activities

A Single Domain–Based Anti-Her2 Antibody Has Potent Antitumor Activities

Xylella fastidiosa: Host Range and Advance in Molecular Identification Techniques

A Review of the Citrus Greening Research and Development Efforts Supported by the Citrus Research and Development Foundation

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