A Review of the Citrus Greening Research and Development Efforts Supported by the Citrus Research and Development Foundation

Earth, Division on

doi:10.17226/25026

Cited by 11 publications

(5 citation statements)

References 155 publications

(242 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…3), lo cual indica una correlación nula de acuerdo con los rangos de la magnitud de correlación. Una situación similar de no correlación significativa de D. citri en lima persa con la temperatura observaron Moreno et al (2008).…”

Section: /9unclassified

Distribución espacio-temporal de adultos de Diaphorina citri Kuwayama en Citrus latifolia en Nayarit, México: implicaciones para su manejo regional

Fuentes

Isiordia-Aquino²,

Urías-López³

et al. 2022

Rev. Colomb. Entomol.

View full text Add to dashboard Cite

La enfermedad de los cítricos conocida como Huanglongbing (HLB) es una de las más importantes a nivel mundial. Desde 2009 hasta la actualidad, México realiza el monitoreo poblacional y control del insecto vector de esta enfermedad, el psílido asiático Diaphorina citri, mediante el uso de insecticidas y enemigos naturales. El objetivo de esta investigación fue estimar la distribución espacio-temporal de las poblaciones de D. citri en lima persa Citrus latifolia bajo un esquema de manejo regional en Nayarit, México. El estudio se llevó a cabo de agosto de 2018 a Julio de 2020 en dos ambientes: cálido subhúmedo y en semicálido subhúmedo. Se estimó la fluctuación temporal y distribución espacial mediante la captura de adultos del psílido en huertos de las dos zonas climáticas. Se utilizaron 2.041 trampas amarillas distribuidas en 1.100 hectáreas, las cuales se revisaron quincenalmente. Se realizaron análisis de varianza del número de adultos entre localidades, por zona climática y entre meses del año. Se estimaron correlaciones entre la población y la temperatura y la precipitación pluvial. La población de adultos de D. citri fue estadísticamente similar entre huertos de una misma zona climática, por lo que se justifica un manejo similar por zona climática. En ambos climas se detectó diferencia significativa de poblaciones de D. citri entre los meses del año. En clima cálido subhúmedo, las poblaciones más altas de adultos de D. citri se registraron en julio-agosto y en clima semicálido subhúmedo, ocurrieron en febrero-marzo y agosto por lo que se requeriría aplicar una medida de control en estos periodos. En este estudio no se detectó correlación significativa entre poblaciones de D. citri con la temperatura ni con la precipitación pluvial. El control de D. citri puede ser regional, diferenciado en cada zona climática y de acuerdo con los meses del año con mayor población.

show abstract

Section: /9unclassified

Distribución espacio-temporal de adultos de Diaphorina citri Kuwayama en Citrus latifolia en Nayarit, México: implicaciones para su manejo regional

Fuentes

Isiordia-Aquino²,

Urías-López³

et al. 2022

Rev. Colomb. Entomol.

View full text Add to dashboard Cite

show abstract

“…After olfactometry assays with CLas-infected psyllids, each tested female was processed in a Tissue Lyzer (Quiagen, Germany) using stainless steel beads and their DNA extracted (Murray and Thompson, 1980). DNA from each single psyllid was analyzed for the presence of CLas (Li et al, 2006) and D. citri DNA (Manjunath et al, 2008).…”

Section: Diaphorina Citri Olfactometric Assaysmentioning

confidence: 99%

Engineered Orange Ectopically Expressing the Arabidopsis β-Caryophyllene Synthase Is Not Attractive to Diaphorina citri, the Vector of the Bacterial Pathogen Associated to Huanglongbing

et al. 2021

View full text Add to dashboard Cite

Huanglongbing (HLB) is a destructive disease, associated with psyllid-transmitted phloem-restricted pathogenic bacteria, which is seriously endangering citriculture worldwide. It affects all citrus species and cultivars regardless of the rootstock used, and despite intensive research in the last decades, there is no effective cure to control either the bacterial species (Candidatus Liberibacter spp.) or their insect vectors (Diaphorina citri and Trioza erytreae). Currently, the best attempts to manage HLB are based on three approaches: (i) reducing the psyllid population by intensive insecticide treatments; (ii) reducing inoculum sources by removing infected trees, and (iii) using nursery-certified healthy plants for replanting. The economic losses caused by HLB (decreased fruit quality, reduced yield, and tree destruction) and the huge environmental costs of disease management seriously threaten the sustainability of the citrus industry in affected regions. Here, we have generated genetically modified sweet orange lines to constitutively emit (E)-β-caryophyllene, a sesquiterpene repellent to D. citri, the main HLB psyllid vector. We demonstrate that this alteration in volatile emission affects behavioral responses of the psyllid in olfactometric and no-choice assays, making them repellent/less attractant to the HLB vector, opening a new alternative for possible HLB control in the field.

show abstract

“…Genetic resistance to different pests and diseases has been achieved by transgenic approaches ( Fagoaga et al, 2001 ; Soler et al, 2012 ; Rodríguez et al, 2014 ; Hao et al, 2016 ; Kobayashi et al, 2017 ; Sandhu et al, 2019 ; De Francesco et al, 2020 ), but any of these new genotypes is been used in the field, probably because of consumers’ rejection to GMOs. Currently HLB, a still incurable citrus disease, is threatening commercial citriculture almost worldwide and it has been proposed that the only chance to fight it is to generate genetically modified resistant cultivars ( Yan et al, 2015 ; McCollum and Baldwin, 2016 ; NASC, 2018 ; Cochrane and Shade, 2019 ; Wang, 2020 ; Alquezar et al, 2021 ). By means of transgenesis, different Cas9 enzymes have been already proved to be able to generate edited knockout citrus mutants ( Jia et al, 2017a , b , 2019 ; Peng et al, 2017 ; LeBlanc et al, 2018 ; Jia and Wang, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…HLB has not yet been cured, and attempts to minimize its damages have huge environmental and economic costs ( Bassanezi et al, 2020 ). It has been proposed that genetically modified HLB-resistant cultivars are the main feasible option to obtain durable and sustainable control of this disease ( Yan et al, 2015 ; McCollum and Baldwin, 2016 ; NASC, 2018 ; Cochrane and Shade, 2019 ; Wang, 2020 ; Alquezar et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Generation of Transfer-DNA-Free Base-Edited Citrus Plants

et al. 2022

View full text Add to dashboard Cite

To recover transgenic citrus plants in the most efficient manner, the use of selection marker genes is essential. In this work, it was shown that the mutated forms of the acetolactate synthase (ALS) gene in combination with the herbicide selection agent imazapyr (IMZ) added to the selection medium may be used to achieve this goal. This approach enables the development of cisgenic regenerants, namely, plants without the incorporation of those bacterial genes currently employed for transgenic selection, and additionally it allows the generation of edited, non-transgenic plants with altered endogenous ALS genes leading to IMZ resistance. In this work, the citrus mutants, in which ALS has been converted into IMZ-resistant forms using a base editor system, were recovered after cocultivation of the explants with Agrobacterium tumefaciens carrying a cytidine deaminase fused to nSpCas9 in the T-DNA and selecting regenerants in the culture medium supplemented with IMZ. Analysis of transgene-free plants indicated that the transient expression of the T-DNA genes was sufficient to induce ALS mutations and thus generate IMZ-resistant shoots at 11.7% frequency. To our knowledge, this is the first report of T-DNA-free edited citrus plants. Although further optimization is required to increase edition efficiency, this methodology will allow generating new citrus varieties with improved organoleptic/agronomic features without the need to use foreign genes.

show abstract

A Review of the Citrus Greening Research and Development Efforts Supported by the Citrus Research and Development Foundation

Cited by 11 publications

References 155 publications

Distribución espacio-temporal de adultos de Diaphorina citri Kuwayama en Citrus latifolia en Nayarit, México: implicaciones para su manejo regional

Distribución espacio-temporal de adultos de Diaphorina citri Kuwayama en Citrus latifolia en Nayarit, México: implicaciones para su manejo regional

Engineered Orange Ectopically Expressing the Arabidopsis β-Caryophyllene Synthase Is Not Attractive to Diaphorina citri, the Vector of the Bacterial Pathogen Associated to Huanglongbing

Generation of Transfer-DNA-Free Base-Edited Citrus Plants

Contact Info

Product

Resources

About