Development of simple sequence repeat (SSR) markers for the assessment of gene flow and genetic diversity in pigeonpea (<i>Cajanus cajan</i>)

Burns, M. J.; Edwards, Keith J.; Newbury, H. J.; Ford‐Lloyd, B. V.; Baggott, C. D.

doi:10.1046/j.1471-8278.2001.00109.x

Cited by 65 publications

(92 citation statements)

References 8 publications

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“…culinaris) were used for PCR amplification to study transferability as well as their utility in genetic diversity analysis of pigeonpea cultivars. The pigeonpea microsatellite markers were based on the sequences published by Burns et al (2001) whereas common bean and lentil SSR markers were Physiol. Mol.…”

Section: Microsatellite Markers and Pcr Amplificationmentioning

confidence: 99%

“…Despite its importance in nutritional security, pigeonpea has not benefited much from the advances made in the field of genomic tools and molecular markers (Datta et al, 2009). As compared to large number of markers in common bean (L'taief et al, 2008;Blair et al, 2003), chickpea (Choudhary et al, 2009;Sethy et al, 2006), and lentil (Hamwieh et al, 2009;Hamwieh et al, 2005), only 109 microsatellite markers are reported so far in pigeonpea (Burns et al, 2001;Odeny et al, 2007;Odeny et al, 2009). Therefore, there is an urgent need to increase the number of polymorphic microsatellite markers in pigeonpea for diversity analysis and mapping of important traits.…”

Section: Introductionmentioning

confidence: 99%

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Cross-genera amplification of informative microsatellite markers from common bean and lentil for the assessment of genetic diversity in pigeonpea

Datta

Mahfooz

Singh

et al. 2010

Physiol Mol Biol Plants

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A total of 24 pigeonpea (Cajanus cajan L. Millspaugh) cultivars representing different maturity groups were evaluated for genetic diversity analysis using 10 pigeonpea specific and 66 cross-genera microsatellite markers. Of the cross-genera microsatellite markers, only 12 showed amplification. A total of 45 alleles were amplified by the 22 markers. Nine markers showed 100 % polymorphism. Markers Lc 14, BMd 48 and CCB 9 amplified maximum number (5) of alleles each. One genotype specific unique band in Pusa 9 was generated by markers CCB 8. Maximum genetic diversity (74 %) was observed between cultivars MA 3 and CO 6, while the minimum diversity (12 %) was observed between NDA 1 and DA 11. The average diversity among the cultivars was estimated to be 45.6 %. SSR primers from pigeonpea were found to be more polymorphic (37 %) as compared to common bean and lentil markers. The arithmetic mean heterozygosity (Hav) and marker index (MI) were found to be 0.014 and 0.03, respectively, indicating the potential of common bean and lentil microsatellite markers for genetic mapping, diversity analysis and genotyping in Cajanus.

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Section: Microsatellite Markers and Pcr Amplificationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cross-genera amplification of informative microsatellite markers from common bean and lentil for the assessment of genetic diversity in pigeonpea

Datta

Mahfooz

Singh

et al. 2010

Physiol Mol Biol Plants

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“…As genetic diversity in cultivated pigeon pea (C. cajan) is low (Burns et al 2001;Yang et al 2006), linkage maps have not been constructed in intraspecific mapping populations so far. Sufficiently large numbers of common markers are required possibly with good SSRs or some other co-dominant markers to establish synteny/colinearity between these maps in future.…”

Section: Discussionmentioning

confidence: 99%

“…As very few (∼10%) polymorphisms have been identified in cultivated pigeon pea lines using a limited number of SSR and DArT markers (Burns et al 2001;Yang et al 2006), an interspecific mapping population was developed by using pigeon pea (C. cajan) accession ICP 28 and ICPW 94 from a wild relative species of pigeon pea (C. scarabaeoides) to avail maximum polymorphism and to generate the first generation linkage map. Here we demonstrate that DArTs can be effectively applied to genetic linkage mapping of pigeon pea and report the first genetic linkage map of pigeon pea.…”

Section: Introductionmentioning

confidence: 99%

The first genetic map of pigeon pea based on diversity arrays technology (DArT) markers

Shi

Saxena

Kulwal

et al. 2011

J Genet

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With an objective to develop a genetic map in pigeon pea (Cajanus spp.), a total of 554 diversity arrays technology (DArT) markers showed polymorphism in a pigeon pea F 2 mapping population of 72 progenies derived from an interspecific cross of ICP 28 (Cajanus cajan) and ICPW 94 (Cajanus scarabaeoides). Approximately 13% of markers did not conform to expected segregation ratio. The total number of DArT marker loci segregating in Mendelian manner was 405 with 73.1% (P > 0.001) of DArT markers having unique segregation patterns. Two groups of genetic maps were generated using DArT markers. While the maternal genetic linkage map had 122 unique DArT maternal marker loci, the paternal genetic linkage map has a total of 172 unique DArT paternal marker loci. The length of these two maps covered 270.0 cM and 451.6 cM, respectively. These are the first genetic linkage maps developed for pigeon pea, and this is the first report of genetic mapping in any grain legume using diversity arrays technology.[Yang S. Y., Saxena R. K., Kulwal P. L., Ash G. J., Dubey A., Harper J. D. I., Upadhyaya H. D., Gothalwal R., Kilian A. and Varshney R. K. 2011 The first genetic map of pigeon pea based on diversity arrays technology (DArT) markers. J. Genet. 90, [103][104][105][106][107][108][109]

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“…Ten SSR primer pairs (Burns et al, 2001) were used for genotyping. Each 25 ml reaction contained 25 ng of genomic DNA, 1 £ PCR buffer (50 mM KCl, 20 mM Tris -HCl (pH 8.4)), 10 pmol of each primer, 2 mM MgCl 2 , 200 nM dNTP, 50 mM dATP and 1U Taq DNA polymerase (Amersham Pharmacia).…”

Section: Ssr Analysismentioning

confidence: 99%

Efficiency of three DNA markers in revealing genetic variation among wild Cajanus species

Aruna

Rao

Sivaramakrishnan

et al. 2008

Plant Genet. Resour.

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Wild relatives of pigeonpea (Cajanus cajan L.) possess many useful genes that can be utilized for crop improvement, most importantly genes for resistance to Helicoverpa armigera, the legume pod borer. The present study aimed at quantifying diversity in a collection of Cajanus scarabaeoides, Cajanus sericeus, Cajanus reticulatus and C. cajan species selected from a wide geographic range using two PCR-based marker systems, amplified fragment length polymorphism (AFLP) and simple sequence repeats (SSRs), and the hybridization-based restriction fragment length polymorphism (RFLP). Polymorphism was higher among the wild accessions than among the cultivated genotypes. Wild and cultivated Cajanus accessions belonging to different species clustered into four distinct major groups largely based on the interspecific differences. C. scarabaeoides accessions derived from same geographical origins formed one group reflecting similar genetic makeup of these accessions. Dendrograms generated using AFLP, RFLP and SSR marker data were comparable with minor clustering differences, which suggests that either method, or a combination of both can be applied to expanded genetic studies in Cajanus. Mantel testing confirmed the congruence between the genetic distances of three markers, indicating that the markers segregated independently, giving similar grouping patterns of all accessions having similar genetic origin.

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Development of simple sequence repeat (SSR) markers for the assessment of gene flow and genetic diversity in pigeonpea (Cajanus cajan)

Cited by 65 publications

References 8 publications

Cross-genera amplification of informative microsatellite markers from common bean and lentil for the assessment of genetic diversity in pigeonpea

Cross-genera amplification of informative microsatellite markers from common bean and lentil for the assessment of genetic diversity in pigeonpea

The first genetic map of pigeon pea based on diversity arrays technology (DArT) markers

Efficiency of three DNA markers in revealing genetic variation among wild Cajanus species

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