Development of simple and rapid HLA-C genotyping method using an oligonucleotide microarray

Abstract

Human Leukocyte Antigen (HLA), called human Major Histocompatibility Complex (MHC), performs the crucial function of antigen presentation in human immunity, and correctly matched HLA antigens improves survival and reduces immunological complications. Therefore, a precise HLA typing method is necessary for successful transplantation between donor and recipient. Until now, many methods have been developed for HLA typing, including serological and DNA-based methods. However, these previous methods have been somewhat limited due to their requirements for a large number of samples and complex experimental procedures. To resolve this problem, in this study, we developed rapid genotyping methods for HLA-C using an oligonucleotide microarray. To evaluate the performance of the HLA-C genotyping microarray, we analyzed 50 unrelated Korean individuals. The genotyping results obtained by the oligonucleotide microarray were identical with those of PCR-SBT and PCR-SSPs, at a low-resolution, 2-digit level. Therefore, this microarray method for genotyping HLA constitutes a rapid, simultaneous, and high-throughput method that can be used as an alternative to current methods.

Development of a photosensitive, high-throughput chip-based superoxide dismutase (SOD) assay to explore the radioprotective activity of herbal plants

“…Different methods and probes (indicators) are used to measure cellular SOD activity. Recent technological developments have shown the potential of chip-based assays over conventional assay platforms (Mahto et al, 2008;Yoon et al, 2008). Based on the data in the present study, the developed chip-based SOD assays can overcome the lacunas in the existing methods (Somashekar and Abshire, 2008).…”

Description of a Remarkable New Skate Species of Leucoraja Malm, 1877 (Rajiformes, Rajidae) from the Southwestern Indian Ocean: Introducing 3D Modeling as an Innovative Tool for the Visualization of Clasper Characters

“…However, due to the large interspecific variation in external characters, the clasper features presently appear to be the most reliable generic characters for the differentiation of Leucoraja from the generally similar genus Rajella Stehmann, 1970 [ 9 ]. Although several studies have called for a reliable phylogenetic hypothesis of skates [ 10 ], the monophyly within these two (and further) rajid genera has not yet been examined comprehensively, neither morphologically nor genetically. Several studies from different fields of research have focused on a single species, particularly the model organism L. erinacea (Mitchill, 1825) [ 11 , 12 , 13 , 14 ], as well as L. naevus (Müller and Henle, 1841) [ 15 ].…”