Development of Sanitation Protocol for Leaf Explants of <i>B. huillensis</i> for <i>in Vitro</i> Culture

Abstract

Brachylaena huillensis (Silver Oak) is a multipurpose timber tree species in the family of Asteraceae. There has been a very high demand for B. huillensis wood and its products leading to overexploitation. B. huillensis regenerates through seedlings. However, it produces seeds with poor germination. Seeds are also difficult to be collected because of small size. Many are eaten by insects and currently there is a lack of seed bank. The facts that have hindered and rendered the natural regeneration of the tree species were uncertain. The present investigation was carried out to develop sanitation protocol of B. huillensis using leaves as explants. Juvenile leaves from the tips of B. huillensis naturally growing seedlings were collected from Bombo West Forest Reserve in Tanzania. The leaves were washed and immersed in NaOCL containing various concentrations levels and two drops of tween 20. There was significant difference between the concentrations levels employed. However, the best results were obtained when leaf explants were immersed in 1.5% v/v NaOCL for ten minutes and later in ethanol for ten seconds and cultured on woody plant media medium containing antifungal (cefotaxime). Genuinely, the protocol is vital and so opens up the way for other subsequent stages for in vitro propagation of B. huillensis.

Effect of Ascorbic Acid, Activated Charcoal and Dark Incubation on Browning Intensity of Saurauia bracteosa In Vitro Culture

“…The explants were immersed in 70% alcohol for 3 minutes and then rinsed using sterile distilled water three times. Afterwards, the leaf explants were immersed in 1.5% NaOCl for 15 minutes (Ndakidemi et al, 2013) and then rinsed using sterile distilled water three times. The sterilized explants were dried in sterilized filter paper on a petridish before further treatments.…”

PENGARUH TEKNIK STERILISASI EXPLAN TERHADAP TINGKAT PEROLEHAN KULTUR JARINGAN AKSENIK RAMIN (Gonystylus bancanus)

“…Penelitian ini menggunakan agen sterilisasi sodium hipoklorit (NaClO), pembentuk dua substansi setelah pelarutan dalam aquadestilasi yaitu asam hipoklorous (HOCl) dan ion hipoklorit yang kurang aktif. Senyawa ini berperan penting dalam oksidasi dan disinfeksi serta dapat lebih menurunkan tingkat kontaminasi bila digunakan bergantian dengan alkohol, dibandingkan tanpa alkohol (Ndakidemi, Mneney, & Ndakidemi, 2013). Namun demikian, beberapa penelitian menunjukkan bahwa kontaminasi tetap terjadi dan terdapat 19 jenis kontaminan bakteri maupun jamur yang teridentifikasi berasosiasi dengan kultur jaringan di berbagai lingkungan laboratorium (Odutayo, Amusa, Okutade, & Ogunsanwo, 2007).…”

Assessment of the Natural Regeneration Status of Irvingia Gabonensis (Bush Mango) in the Kwano Forest of Gashaka Gumti National Park, Nigeria

“…In other words, the forest was rich with abundance of I. gabonensis species, but due to poverty, economic hardship, and human search for non-timber forest products as alternative means of survival, I. gabonensis species has likely been overexploited or affected by wildfire. Therefore, there is a need to carry out a natural regeneration status to ascertain its survival rate in the near future (Ndakidemi and Ndakidemi 2013). As such the study intends; to determine the abundance of I. gabonensis species with respect to age classes; seedlings, saplings, poles, and matured, to investigate the vegetative composition and also the status of natural regeneration on different ecological zones of the forest and to also determine indigenous knowledge on I. gabonensis by locals around the forest (Adekunle, 2007).…”