Development of RT-PCR Based Diagnosis of SARS-CoV-2

Abstract

In the 2020, COVID-19 pandemic disease created an havoc situation world widely and mainly caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). It has been challenging task for researchers, scientists and medico-pharmaceutical organisations to find out rapid and reliable diagnosis methods. Among the all testing services, a Reverse Transcription Polymerase Chain Reaction (RT-PCR) is the more accurate, rapid and authenticated molecular technique used for most of the diagnosis of major diseases. It has been a global priority to fix the rapid diagnosis method to combat against the pandemic COVID-19. Thus, the present chapter mainly focussing on the progress of RT-PCR method development though various processes of data collection on isolation of whole genome sequence, its primer and method designing. In this scenario, India suddenly become the global leader for vaccine development and hence the challenges and RT-PCR kit development in India and rest of the world has been be discussed. World wide many Government and private agencies and industries have taken an initiative for diagnosis of SARS-CoV-2 hence this chapter also summarised the scope of RT-PCR to combat pandemic situation in future.

Development of Nested PCR for SARS-CoV-2 Detection and Its Application for Diagnosis of Active Infection in Cats

“…In addition, the RT-PCR results may be influenced by the type of collected specimen, the clinical symptoms of the patient and the period of time between sampling and the onset of symptoms [24,25]. Regarding nested PCR, whether conventional or real-time, cross-contamination of the samples was outlined as a drawback [26]. However, we deem that when the aseptic working principles in a virology laboratory are observed, the risk of contamination is minimized.…”

Viewpoint on relief of COVID-19 burden in India by administration of system to break the chain of SARS-CoV-2 at the grass-roots level

“…6. To create a special room for corona testing (quick test of rapid antigen Test and confirmatory RT-PCR test [11] . 7.…”

Factor and Rasch Analysis on COVID-19 Genetics Literacy Assessment Instrument

“…Related to the reproduction of genetic material, there is a statement dealing with the diagnosis of COVID-19. An accurate way of diagnosing someone infected by COVID-19 is by using RT-PCR (Patankar & Zambare, 2021;Torretta et al, 2021). The RT-PCR identifies the presence of nucleic acid sequences possessed by certain living things or viruses to determine their presence (Kralik & Ricchi, 2017;Zauli, 2020).…”

“…Studying the genes of the SARS-CoV-2, scientists were able to determine the function of each of these genes (Naqvi et al, 2020). They were also capable of designing primers and RT-PCR procedures to diagnose suspected COVID-19 patients (Patankar & Zambare, 2021;Torretta et al, 2021). Together with several pharmaceutical companies, scientists are also developing vaccines based on the SARS-CoV-2 genetic code (Borah et al, 2021).…”