Development of rice introgression lines with brown planthopper resistance and KDML105 grain quality characteristics through marker-assisted selection

Jairin, Jirapong; Teangdeerith, Sanguan; Leelagud, Phikul; Kothcharerk, Jate; Sansen, Kalaya; Mingxuan, Yi,; Vanavichit, Apichart; Toojinda, Theerayut

doi:10.1016/j.fcr.2008.09.009

Cited by 71 publications

(56 citation statements)

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“…Sharma et al (2004) succeeded in performing a molecular markerassisted pyramiding of two BPH resistance genes, Bph1 and bph2, into a japonica line. Our recent study was also successful in introducing Bph3 into a Thai aromatic rice cultivar KDML105 using the MAS approach (Jairin et al 2009). The SSR markers linked to the bph4 locus in this study would also be useful for MAS in BPH-resistant breeding programs.…”

Section: Discussionmentioning

confidence: 99%

Detection of a brown planthopper resistance gene bph4 at the same chromosomal position of Bph3 using two different genetic backgrounds of rice

et al. 2010

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The brown planthopper (BPH) resistance gene bph4 has previously been assigned on the short arm of rice chromosome 6. However, the map position of the gene could not be determined. To detect the bph4 locus, 15 polymorphic simple sequence repeat (SSR) markers covering genetic distance of 0.0-63.4 cM on chromosome 6 were used to survey 15 BPH resistant (R) and susceptible (S) individuals from each of the 95 and 78 F 2 populations derived from crosses of TN1/Babawee and Babawee/KDML105, respectively. One SSR marker, RM586, was associated with the R and S from the F 2 populations. Additional markers surrounding the RM586 locus were examined to define the location of bph4. From the genetic linkage map and QTL analysis of 95 and 78 F 2 individuals, the bph4 locus was mapped at the same chromosomal region of Bph3 between two flanking markers RM589 and RM586. Markers linked to the resistance gene explained 58.8-70.1% of the phenotypic variations and can be used for marker-assisted selection in BPH-resistant breeding programs. In addition, our experiment provides evidence that a recessive gene could behave as a dominant gene under different genetic backgrounds.

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Section: Discussionmentioning

confidence: 99%

Detection of a brown planthopper resistance gene bph4 at the same chromosomal position of Bph3 using two different genetic backgrounds of rice

et al. 2010

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“…The understanding of the molecular basis of resistance to BPH was greatly advanced with the cloning and characterization of several BPH resistance genes. Among these, a broad spectrum BPH32 gene originally derived from PTB33 and RH has been extensively studied and introgressed into numerous commercial rice varieties with desirable backgrounds [6] [8]. New rice varieties with BPH resistance have been developed and introduced to farmers [1].…”

Section: Discussionmentioning

confidence: 99%

“…An introgression line UBN03078 was developed through successive introgression backcrossing (BC3) and MAS with a single introgressed segment [6]. The BPH resistance gene BPH32 from RH, the Sri Lankan landrace rice, was introgressed to KDML105 (KD), the aromatic rice known as Jasmine rice.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Although the mechanism of losing resistance at the heading stage is not well understood, it has been hypothesized that the resistance gene BPH32 may be poorly expressed in the uppermost internodes of heading rice plants [11]. To verify that the low-level expression of BPH32 causes the loss of resistance in the uppermost internode, an introgression line UBN03078-101-342-4-1-141 (UBN03078) carrying BPH32 from RH [6] was used for gene expression analysis in this study. The results from our study will provide a starting point for future studies aimed at understanding the factors or mechanisms that depress the expression of the BPH32 gene in rice panicle.…”

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Loss of Resistance to <i>Nilaparvata lugens</i> May Be Due to the Low-Level Expression of <i>BPH32 </i>in Rice Panicles at the Heading Stage

Jairin¹,

Leelagud²,

Saejueng³

et al. 2017

AJPS

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The brown planthopper (BPH), Nilaparvata lugens (Stål), is the most important insect pest of rice in Asia. Host plant resistance is one of the strategies currently used to control BPH. The resistant rice cultivar Rathu Heenati (RH) carrying the BPH3 gene (recently renamed as "BPH32") remains effective despite more than 30 years of deployment. RH has been determined to be resistant against BPH at all growth stages. However, we observed that BPH could feed on panicles but not on the leaf sheaths of RH. The resistance gene BPH32 was introduced into KDML105 through marker-assisted selection, and the introgression line UBN03078 was developed. This rice line was used to observe the patterns of target gene's regulation. A low-level expression of BPH32 on panicles has been hypothesized to cause susceptibility in UBN03078 at the heading stage. Findings from our gene expression analysis support the hypothesis that the resistance gene was down regulated in the uppermost internodes compared with the leaf sheaths of the heading rice plant. This phenomenon may allow BPH to feed on the panicles of the resistant plants, but this requires further investigation.

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“…A number of major BPH-resistance genes have been identified from indica rice cultivars (Chen et al 2006, Kabir and Khush 1988, Khush 1979, Khush et al 1985, Nemoto et al 1989, Sun et al 2005. The development of near-isogenic lines (NILs) and pyramided lines (PYLs) carrying one or several resistance genes has been performed (Jairin et al 2009, Sharma et al 2004 and is a most effective manner to facilitate the use of the BPH-resistance genes or of quantitative trait loci (QTLs) for future rice improvement.…”

Section: Introductionmentioning

confidence: 99%

Development of near-isogenic lines for BPH25(t) and BPH26(t), which confer resistance to the brown planthopper, Nilaparvata lugens (Stal.) in indica rice 'ADR52'

et al. 2010

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Development of near-isogenic lines for BPH25(t) and BPH26(t), which confer resistance to the brown planthopper, Nilaparvata lugens (Stål.) in indica rice 'ADR52' Asanori Yara 1) , Cong Nguyen Phi 1,2) , Masaya Matsumura 3) , Atsushi Yoshimura 1) and Hideshi Yasui* 1) The brown planthopper [BPH; Nilaparvata lugens (Stål.)] is one of the most destructive insect pests in Asian rice-growing areas. Two genes conferring resistance to BPH, BPH25(t) and BPH26(t) derived from a BPHresistant indica rice cultivar, Oryza sativa ADR52, have been identified. However, they are linked to genes conferring late heading and hybrid spikelet sterility. To eliminate these unfavorable traits (linkage drag), we generated BC 6 F 1 populations carrying BPH25(t) or BPH26(t) in a BPH-susceptible japonica cultivar, Taichung 65, through marker-assisted selection. We selected three near-isogenic lines (NILs) carrying BPH25(t) without late heading date and one NIL carrying BPH26(t) without spikelet sterility from BC 6 F 2 progeny that showed between 96.3 and 99.8% of the Taichung 65 genetic background through whole-genome survey. In antibiosis testing, the rates of surviving insects and of females with swollen abdomens were lower on the NILs than on Taichung 65, indicating that bph25(t) and Bph26(t) alleles from ADR52 controlled the resistance to BPH. The NILs will serve as useful resources for (1) monitoring BPH virulence and for (2) increasing resistance to BPH.

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Development of rice introgression lines with brown planthopper resistance and KDML105 grain quality characteristics through marker-assisted selection

Cited by 71 publications

References 30 publications

Detection of a brown planthopper resistance gene bph4 at the same chromosomal position of Bph3 using two different genetic backgrounds of rice

Detection of a brown planthopper resistance gene bph4 at the same chromosomal position of Bph3 using two different genetic backgrounds of rice

Loss of Resistance to <i>Nilaparvata lugens</i> May Be Due to the Low-Level Expression of <i>BPH32 </i>in Rice Panicles at the Heading Stage

Development of near-isogenic lines for BPH25(t) and BPH26(t), which confer resistance to the brown planthopper, Nilaparvata lugens (Stal.) in indica rice 'ADR52'

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Development of rice introgression lines with brown planthopper resistance and KDML105 grain quality characteristics through marker-assisted selection

Cited by 71 publications

References 30 publications

Detection of a brown planthopper resistance gene bph4 at the same chromosomal position of Bph3 using two different genetic backgrounds of rice

Detection of a brown planthopper resistance gene bph4 at the same chromosomal position of Bph3 using two different genetic backgrounds of rice

Loss of Resistance to &lt;i&gt;Nilaparvata lugens&lt;/i&gt; May Be Due to the Low-Level Expression of &lt;i&gt;BPH32 &lt;/i&gt;in Rice Panicles at the Heading Stage

Development of near-isogenic lines for BPH25(t) and BPH26(t), which confer resistance to the brown planthopper, Nilaparvata lugens (Stal.) in indica rice 'ADR52'

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Loss of Resistance to <i>Nilaparvata lugens</i> May Be Due to the Low-Level Expression of <i>BPH32 </i>in Rice Panicles at the Heading Stage