Development of Protective Immunity against Inactivated Iranian Isolate of Foot-and-Mouth Disease Virus Type O/IRN/2007 Using Gamma Ray-Irradiated Vaccine on BALB/c Mice and Guinea Pigs

Motamedi-Sedeh, Farahnaz; Soleimanjahi, Hoorieh; Jalilian, Amir Reza; Mahravani, Homayoon; Shafaee, Kamalodin; Sotoodeh, Masood; Taherkarami, Hamdolah; Jairani, Faramarz

doi:10.1159/000433538

Cited by 12 publications

(9 citation statements)

References 27 publications

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“…Motamedi-Sedeh showed that radiation can keep the antigenic characteristic of inactivated virus antigen and thus it is a good way for the immunization of animals to induce neutralizing antibody titers and cytokine concentration such as IL-2 and IFNγ [33]. Meng explains IFN-α can to protect chickens against AIV [34].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Immune Responses and Histopathological Effects against Gamma Irradiated Avian Influenza (Sub type H9N2) Vaccine on Broiler Chicken

Javan

Motamedi-Sedeh²,

Dezfoolian

et al. 2020

Braz. arch. biol. technol.

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Vaccination is a good strategy for the prevention of avian influenza virus. In this research Gamma Irradiated Avian Influenza (Sub type H9N2) Vaccine (GAIV) was prepared by 30 kGy irradiation and used for vaccination of broiler chickens. The purpose was a comparison of immune responses in the two routes of administration for the GAIV vaccine; intranasal and subcutaneously, use of Montanide ISA70 and Trehalose accompanied with irradiated vaccine and compare with formalin vaccine. The Influenza Virus A/Chicken/IRN/Ghazvin/2001/H9N2 was irradiated and used for vaccine formulation, and formalin inactivated AIV was used as conventional vaccine. Chickens were vaccinated by GAIV with and without Trehalose, GAIV and formalin vaccines with ISA70, two routes of administration were intranasal and subcutaneously. All the vaccinated chickens showed a significant increase in antibody titration. The most significant increase of antibody titration was in irradiated vaccine plus Trehalose groups intranasal and subcutaneously. After the first and second intranasal vaccination, the amount of IFN-gamma increased in the irradiated vaccine plus Trehalose group compared to other groups. However, most of the vaccinated groups did not show any significant increase of IFN-α concentration. Histopathological examination revealed lymphocyte infiltration (++), foci dispersed of hemorrhage and edema in intranasal vaccination groups and in addition to these, thickening of alveolar septa was observed in the injection groups. GAIV vaccine can be a good candidate for vaccine preparation, and Trehalose as a stabilizer protects viral antigenic proteins, also makes more absorbance of antigen by the inhalation route. In vaccinated chickens the ulcers in injected vaccines were lower than intranasal vaccines. HIGHLIGHTS  Gamma Irradiated Avian Influenza (H9N2) Vaccine was prepared by 30 kGy irradiation.  Broiler chickens were vaccinated by two routs; intranasal and subcutaneously.  All the vaccinated chickens showed a significant increase in antibody titration.  The most increasing of immune responses was in irradiated vaccine plus Trehalose. 2 Motamedi-Sedeh, F.; et al.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Immune Responses and Histopathological Effects against Gamma Irradiated Avian Influenza (Sub type H9N2) Vaccine on Broiler Chicken

Javan

Motamedi-Sedeh²,

Dezfoolian

et al. 2020

Braz. arch. biol. technol.

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“…The LD50 of the live virus stock (WSSV/IRN/1/2010) was calculated at 10 5.4 /ml by the Karber formula [23,27]. D 10 value and the optimum dose of gamma ray for viral inactivation were calculated according to the dose/response curve for the irradiated and unirradiated viral samples at 2.56 and 15 kGy, respectively (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…SAL is in general a value of 10 -6 (a one in a million chance of having live microorganisms) [29]. When inactivated vaccine are going to be prepared, the most important parameter is the minimum inactivation endpoint which is dependent on parameters such as the inactivation rate, dose of irradiation and virus titer per volume unit [27]. Ionizing radiations such as gamma rays can damage the molecular structure of viral nucleic acids that cause virucidal effect [28,30,31].…”

Section: Discussionmentioning

confidence: 99%

Immunization of Litopenaeus vannamei shrimp against white spot syndrome virus (WSSV) by gamma-irradiated WSSV plus Vibrio paraheomolyticus

Motamedi-Sedeh

Afsharnasab²,

Heidarieh

2015

vacres

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Introduction: White spot syndrome virus (WSSV) is one of the most deadly infectious pathogens of the shrimp culture industry. Neither effective vaccines nor efficient treatments are currently available for this disease. Vibrio species are well known dominant bacterial pathogens in the shrimp ponds. As facultative pathogenic bacteria, it is possible that Vibrio spp. along with WSSV to co-infect the shrimp species such as Litopenaeus vannamei. The aim of this study was to investigate the co-administration of gamma-irradiated Vibrio paraheomolyticus as a kind of probiotic and immune-stimulator with gamma-irradiated and inactivated WSSV as an intramuscular vaccine for protection of L. vannamei against WSSV infection. Methods: WSSV was isolated from the infected shrimp samples and multiplied in Astacus leptodactylus crayfish. Titration of WSSV was obtained in post-larvae as 10 5.4 LD 50 /ml. The virus was irradiated where D 10 value and optimum dose of gamma ray were calculated to be 2.56 and 15 kGy, respectively. The gamma-irradiated WSSV samples, named GI-WSSV were used as a vaccine to immunize L. vannamei shrimps. The freeze-dried V. paraheomolyticus cultures, inactivated by gamma ray (8 kGy) were named GI-V.P and used as a probiotic. Results: Protective dose50 was calculated as 5.61 and 7.94 for the shrimp groups which were vaccinated by GI-WSSV vaccine and GI-WSSV vaccine + GI-V.P, respectively. Calculated RPS values were 73.3%, 86.66% and 26.66% for the GI-WSSV vaccine, GI-WSSV + GI-V.P and isolated probiotic groups, respectively. Significant differences in cumulative mortalities were observed between the vaccination groups and the positive control group (P < 0.05). No significant difference was observed in cumulative mortalities between the two vaccination groups (P > 0.05). Conclusion: GI-WSSV vaccine can induce immune responses in shrimps infected with WSSV and probiotic GI-V.P enhances these responses.

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“…On the other hand, the electromagnetic (EM) spectrum between the UV (i.e., 10 18 Hz) and the X-ray radiation (i.e., 10 19 Hz) is very sensitive to molecular vibration between groups of atoms in macromolecules [30]. Since the resonance absorption energy depends on the protein backbone molecular structure of the amino acids, then each molecule produces its own vibration resonance frequency signature which may lead to the breaking up of the protein structure at speci c position along its backbone [31,32].…”

Section: Introductionmentioning

confidence: 99%

Energetic photons applied to COVID-19 Coronavirus S2 subunit of spike glycoprotein for host cell entry inhabitation: An alternative approach for conventional vaccination

Khattari

AlSayed²,

Afaneh

et al. 2021

Preprint

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We have investigated numerically the ability to inhibit spike protein from SARS-CoV-2 to attach and inter the host cell when exposed to energetic photons. The Geometric Progression fitting method have been adopted to calculate the equivalent atomic number for photon energy absorption (Zeq), exposure and absorption buildup factors in the energy range E∈ [15–300] keV for the S2-subunit in the spike protein. The buildup factors have shown a peak value at adsorption resonance energy between 36–60 eV per amino acid of the S2-subunit which depends on the mean free path of the photon within the protein structure and the type of mutation. The resonance energies (between UV and X-ray range) have been found to depend on the protein molecular composition. This opens the possibility of using energetic photons to break up the S2-subunit into small fragments. Our results may contribute to the continues racing for finding noninvasive technique for medical trials using radiotherapy treatment for the COVID-19 virus.

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Development of Protective Immunity against Inactivated Iranian Isolate of Foot-and-Mouth Disease Virus Type O/IRN/2007 Using Gamma Ray-Irradiated Vaccine on BALB/c Mice and Guinea Pigs

Cited by 12 publications

References 27 publications

Evaluation of Immune Responses and Histopathological Effects against Gamma Irradiated Avian Influenza (Sub type H9N2) Vaccine on Broiler Chicken

Evaluation of Immune Responses and Histopathological Effects against Gamma Irradiated Avian Influenza (Sub type H9N2) Vaccine on Broiler Chicken

Immunization of Litopenaeus vannamei shrimp against white spot syndrome virus (WSSV) by gamma-irradiated WSSV plus Vibrio paraheomolyticus

Energetic photons applied to COVID-19 Coronavirus S2 subunit of spike glycoprotein for host cell entry inhabitation: An alternative approach for conventional vaccination

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