Development of Practical Media and Fermentative Technique for Mass Cultivation from Agricultural and Livestock Microorganism

Abstract

Agricultural and livestock microorganisms including bacteria, yeast and fungi have been used as eco-friendly biological agent in plant growth promoting, disease suppression and odor reduction. In this study, we have optimized culture conditions of three species, such as Bacillus subtilis, Saccharomyces cerevisiae and Lactobacillus casei that could act as effective biological agent by producing growth-promoting factors to crops. They also reduce harmful odors generated from livestock manure. We made up the optimal media recipes for Bacillus subtilis, Saccharomyces cerevisae and Lactobacillus casei named as MMS (glucose 0.5%, soy bean flour 0.8%, NaCl 0.15%, K 2 HPO 4 0.25%, Na 2 CO 3 0.05%, MgSO 4 0.1%), YSM (dextrose 1%, soytone 0.5%, yeast extract 0.5%) and LSM (glucose 2%, soytone 0.5%, casein milk 0.5%, NaAc 0.5%, KH 2 PO 4 0.2%, MgSO 4 0.002%, MnSO 4 0.05%), respectively. The culture period of three microorganisms were determined on 30 hours in 500 L fermenter. In addition, the maximal cell growth of Bacillus subtilis, Saccharomyces cerevisae and Lactobacillus casei was measured as 1.4×10 9 cfu/mL, 6.4×10 8 cfu/mL, 3.3×10 9 cfu/mL, respectively. From above results, we represent that development of practical media and cultural techniques can economize heavily the cost for mass production of agricultural and livestock microorganisms.

Development of Spectinabilin Over-Producing Mutant Streptomyces sp. S-N87 having Nematicidal Activities

“…As a result, WT and S-N87 strains produced spectinabilin at 198 and 2,270 mg/L, respectively. As mass fermentation is essential for the industrial development of microorganisms (Park et al, 2018), this study con rms that the spectinabilin over-production of S-N87 was maintained more than tenfold even after mass fermentation.. After the improvement of the strain progresses, it is essential to determine with optimal conditions. The development of a commercially viable process requires (1) optimisation of the fermentation medium composition with low-cost nutrients, (2) optimisation of the process parameters, and (3) ease of use (Savergave et al, 2011).…”

Mass Cultivation of Rhodococcus sp. 3-2, a Carbendazim-Degrading Microorganism, and Development of Microbial Agents

“…Key words: Benomyl, Biodegradation, Mass production, Microbe, Residual pesticide 서 론 최근 안전한 먹거리에 대한 관심이 증가하고, 지속 가능한 농산업에 대한 필요성이 대두되면서 유해한 화학 잔류물이 없 는 농산품에 대한 수요가 증가하고 있다 [1]. 한편 지속가능한 산업에 대한 요구가 가속화됨으로서 농축산업분야에서 비료와 농약 사용량의 제한을 받을 전망이다 [2].…”