Development of pollen germination medium to test pollen viability of eggplant and its wild species

Jayaprakash, P.; Sheeba, D.; Vikas, V. K.; Sivasamy, M.; Sabesan, T.

doi:10.5958/0974-0112.2018.00041.5

Cited by 6 publications

(3 citation statements)

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“…The basic germination medium contained 100 g L −1 sucrose, 100 mg L −1 boric acid, 300 mg L −1 calcium nitrate, 200 mg L −1 magnesium sulfate, and 100 mg L −1 potassium nitrate in distilled water. Since then, the Brewbaker and Kwack media have become one of the standard germination media for in vitro pollen germination experiments, and several modifications have been made to optimize the medium for specific plant types [ 36 , 37 , 38 , 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

Novel Metrics to Characterize In Vitro Pollen Tube Growth Performance of Apple Cultivars

Roeder

Serra

Musacchi

2021

Plants

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In vitro germination assays are frequently used in screening trials to evaluate the pollen viability of pollinizers. To be effective, screening trials must have defined threshold criteria, from which individuals can then be assessed. However, despite decades of research on pollen viability, no established threshold is available to categorize apple cultivars based on their in vitro pollen tube lengths. This study aimed to identify and characterize the subgroups of cultivars based on their pollen tube growth performance. In vitro pollen tube lengths of 41 individuals were determined by incubating samples on artificial germination media at 15 and 25 °C. A six-number summary statistic was calculated, and hierarchical clustering on principal component (HCPC) analysis was used to determine and characterize subgroups. Furthermore, a decision tree model was used to predict class membership for future datasets. HCPC analysis partitioned the 41 individuals into three subgroups with different performances. The decision tree quickly predicted the cluster membership based on the second quartile at 15 °C and the third quartile at 25 °C. The thresholds from the decision tree can be used to characterize new observations. The use of the methods will be demonstrated using a case study with 29 apple accessions.

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Section: Discussionmentioning

confidence: 99%

Novel Metrics to Characterize In Vitro Pollen Tube Growth Performance of Apple Cultivars

Roeder

Serra

Musacchi

2021

Plants

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“…The medium is generally composed of sugar and ionic salts, such as calcium nitrate, magnesium sulfate and boric acid (1,28,30,50). Organic supplements such as polyethylene glycol, vitamins, amino acids and plant growth hormones were also incorporated to increase the rate of pollen germination (51)(52)(53). BK (30) medium is the most widely used pollen germination media (PGM) which was tested and was found to be suitable in 86 plant species (30,54).…”

Section: Optimization Of Pollen Germination Medium (Pgm) In Rice Bean (V Umbellata)mentioning

confidence: 99%

Pollen viability and incompatibility in indigenous rice bean (Vigna umbellata (Thunb.) Ohwi & Ohashi)

et al. 2021

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Pollen viability, germination and compatibility are essential in determining the success of pollination and seed setting of high-valued crops. Rice bean (Vigna umbellata (Thunb.) Ohwi & Ohashi) is an underutilized and unexplored indigenous legume with high potential for commercial production. In this study, pollen quality, viability, germination rate and incompatibility among selected six rice bean (V. umbellata) accessions from Barili, Cebu, Philippines were evaluated to determine the barriers and effective pollination habit for increased productivity while retaining the important traits, including high tolerance in poor soils, superior climatic resilience and resistance to pest and diseases. Results of acetocarmine calorimetric assay showed that rice beans’ (V. umbellata) pollens are highly viable, with accessions VU 004 (56.33 ± 4.91%) and VU 007 (54.34 ± 4.53%) having the optimum viability rate. Brewbaker and Kwack medium treated with 0.2 g.l-1 and 0.3 g.l-1 boric acid (H3BO4) enhanced the germination rate in vitro (11.56 ± 5.53% and 9.47 ± 6.50% respectively). Bud (14.96 ± 1.53%) and post-anthesis pollens (10.28 ± 0.94%) have optimum germination rate in 0.2 g.l-1 boric acid media, while anthesis pollens are suitable in media supplemented with sucrose and boric acid alone (12.20 ± 1.50%) and with 0.1 g.l-1 myo-inositol supplementation (8.49 ± 1.86%). Pollination test revealed that rice bean accessions have high self-compatibility (50.76 + 3.45%) and low cross-compatibility (26.57 + 2.49%). The findings provide an important background in understanding the pollen quality and intraspecific interaction among indigenous rice bean (V. umbellata) accessions in Barili, Cebu to improve production and hybridization.

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“…Firstly, information on PGM requirements for a species in question is usually extremely scattered in works published in very different journals and/or years. Secondly, although numerous protocols describe methods to induce PG, they are not applicable to all species as they are strongly biased either to model species, such as Arabidopsis thaliana (Boavida and McCormick, 2007;Rodriguez-Enriquez et al, 2013) or domesticated plant species, their cultivars and wild relatives (Roberts et al, 1983;Cheng and Mcomb, 1992;Jayaprakash et al, 2018). In contrast, PG studies for any given wild species are limited (Mortenson et al, 1964;Fernando et al, 2001;Sorkheh et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

A Compendium of in vitro Germination Media for Pollen Research

Tushabe

Rosbakh

2021

Front. Plant Sci.

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The correct choice of in vitro pollen germination media (PGM) is crucial in basic and applied pollen research. However, the methodological gaps (e.g., strong focus of current research on model species and cultivated plants along with the lack of general rules for developing a PGM) makes experimenting with pollen difficult. We closed these gaps by compiling a compendium of optimized in vitro PGM recipes from more than 1800 articles published in English, German, and Russian from 1926 to 2019. The compendium includes 1572 PGM recipes successfully used to germinate pollen grains or produce pollen tubes in 816 species representing 412 genera and 114 families (both monocots and dicots). Among the 110 components recorded from the different PGM recipes, sucrose (89% of species), H3BO3 (77%), Ca2+ (59%), Mg2+ (44%), and K+ (39%) were the most commonly used PGM components. PGM pH was reported in 35% of all studies reviewed. Also, we identified some general rules for creating PGM for various groups of species differing in area of research (wild and cultivated species), phylogenetic relatedness (angiosperms vs. gymnosperms, dicots vs. monocots), pollen physiology (bi- and tri-cellular), biochemistry (starchy vs. starchless pollen grains), and stigma properties (dry vs. wet), and compared the component requirements. Sucrose, calcium, and magnesium concentrations were significantly different across most categories indicating that pollen sensitivity to sugar and mineral requirements in PGM is highly group-specific and should be accounted for when composing new PGM. This compendium is an important data resource on PGM and can facilitate future pollen research.

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Development of pollen germination medium to test pollen viability of eggplant and its wild species

Cited by 6 publications

References 0 publications

Novel Metrics to Characterize In Vitro Pollen Tube Growth Performance of Apple Cultivars

Novel Metrics to Characterize In Vitro Pollen Tube Growth Performance of Apple Cultivars

Pollen viability and incompatibility in indigenous rice bean (Vigna umbellata (Thunb.) Ohwi & Ohashi)

A Compendium of in vitro Germination Media for Pollen Research

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