Development of Pilot Scale Process for Mass Production of Lilium Bulblets in Vitro

Reduced temperature, elevated sucrose, continuous light and gibberellic acid promote corm formation in Watsonia vanderspuyiae

“…This in turn depends on the species and explant type that is being used. For example, 6% or 9% sucrose was effective for Narcissus jonquilla shoot explants (Chow et al 1992), 2% for Lilium longiflorum bulbscales (Nhut 1998), 3% for Lilium 'Casablanca' bulbscales (Lim et al 1998), 4% for Lilium rubellum leaf explants (Niimi and Onozawa 1979) and 6% for Dierama luteoalbidum shoot explants (Madubanya et al 2006). Even different cultivars of the same genus can have differing optimum sugar requirements for storage organ formation.…”

Application of bioreactor systems for large scale production of horticultural and medicinal plants

“…Ginseng tissue culture was first documented in 1964 (Luo et al, 1964); since then numerous studies have been reported (as reviewed by Wu and Zhong, 1999). The large-scale suspension culture of ginseng cells was first reported by Yasuda et al (1972).…”

“…Numerous studies have been reported on regeneration of bulblets from excised lily bulb scales (Robb, 1957;Hackett, 1969;Allen, 1974;Anderson, 1977;Novak and Petru, 1981;Takayama and Misawa, 1983;Varshney et al, 2000) and it is now the current commercial method used for vegetative propagation of lilies. In our previous study, we reported a two-stage culture process of Lilium micropropagation (bulblet formation and then their development) in 5-20 l batches of non-stirred bioreactors within 60 days (Lim et al, 1998;Kim, 1999. The fed-batch culture system using a bioreactor was used for the mass production of Lilium bulblets by Seon et al (2000).…”

Bioreactor systems for micropropagation of plants: present scenario and future prospects

“…Features such as high specific power input, high energy dissipation rate, turbulence around the agitator, and shear may damage the cultured plant tissues ( Huang and McDonald, 2009 ). Nevertheless, mechanically agitated bioreactors have been used to culture bulblets of Lilium auratum and shoot primordia of Stevia rebaudiana ( Takayama and Akita, 1994 ; Lim et al., 1998 ). Severe damage to shoots/propagules have been experienced owing to the high shear stress caused by mechanical agitation.…”