Development of photosynthetic sutures for the local delivery of oxygen and recombinant growth factors in wounds

Centeno-Cerdas, Carolina; Jarquín-Cordero, Montserrat; Chávez, Myra N.; Hopfner, Úrsula; Holmes, Christopher P.; Schmauß, Daniel; Machens, Hans‐Günther; Nickelsen, Jörg; Egaña, José Tomás

doi:10.1016/j.actbio.2018.09.060

Cited by 62 publications

(49 citation statements)

References 55 publications

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“…In this study, they also used immunocompetent hairless female Skh1 mice to prove the safety and biocompatibility of the algal cells over an extended length of time. Finally, the concepts of photosynthetic biomaterials and photosynthetic gene therapy were implemented by the same group to create photosynthetic sutures for the local delivery of oxygen and recombinant growth factors to incisional wounds ( Centeno-Cerdas et al, 2018 ).…”

Section: Photosynthetic Biomaterials For Tissue Engineering and Regenmentioning

confidence: 99%

“…The authors showed that overexpression of recombinant hVEGF by these algal cells following their implantation in immunocompetent mice promoted endothelial cell migration and blood vessel formation, whereas blood vessel ingrowth was observed after injection of the transgenic microalgae into the yolk sac of zebrafish embryos. In the second study, Centeno-Cerdas et al (2018) tested the hypothesis that seeding of absorbable sutures with genetically modified C. reinhardtii would promote wound healing immediately after surgical closure. Here, the authors demonstrated that the photosynthetic sutures were indeed capable of secreting significant amounts of bio-functional recombinant hVEGF, platelet-derived growth factor B (hPDGF-B) and stromal cell-derived factor-1 (hSDF-1), as well as releasing substantial levels of oxygen upon exposure to light ( Centeno-Cerdas et al, 2018 ).…”

Section: Transgenic Photosynthetic Organismsmentioning

confidence: 99%

“…In the second study, Centeno-Cerdas et al (2018) tested the hypothesis that seeding of absorbable sutures with genetically modified C. reinhardtii would promote wound healing immediately after surgical closure. Here, the authors demonstrated that the photosynthetic sutures were indeed capable of secreting significant amounts of bio-functional recombinant hVEGF, platelet-derived growth factor B (hPDGF-B) and stromal cell-derived factor-1 (hSDF-1), as well as releasing substantial levels of oxygen upon exposure to light ( Centeno-Cerdas et al, 2018 ). Finally, Jarquín-Cordero et al (2020) recently established an optimized platform for the secretion of alga-derived human growth factors and described the potentiating angiogenic effect of the combinatorial application of C. reinhardtii transgenic strains.…”

Section: Transgenic Photosynthetic Organismsmentioning

confidence: 99%

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Photosymbiosis for Biomedical Applications

Chávez

Moellhoff

Schenck

et al. 2020

Front. Bioeng. Biotechnol.

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Without the sustained provision of adequate levels of oxygen by the cardiovascular system, the tissues of higher animals are incapable of maintaining normal metabolic activity, and hence cannot survive. The consequence of this evolutionarily suboptimal design is that humans are dependent on cardiovascular perfusion, and therefore highly susceptible to alterations in its normal function. However, hope may be at hand. “Photosynthetic strategies,” based on the recognition that photosynthesis is the source of all oxygen, offer a revolutionary and promising solution to pathologies related to tissue hypoxia. These approaches, which have been under development over the past 20 years, seek to harness photosynthetic microorganisms as a local and controllable source of oxygen to circumvent the need for blood perfusion to sustain tissue survival. To date, their applications extend from the in vitro creation of artificial human tissues to the photosynthetic maintenance of oxygen-deprived organs both in vivo and ex vivo , while their potential use in other medical approaches has just begun to be explored. This review provides an overview of the state of the art of photosynthetic technologies and its innovative applications, as well as an expert assessment of the major challenges and how they can be addressed.

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Section: Photosynthetic Biomaterials For Tissue Engineering and Regenmentioning

confidence: 99%

Section: Transgenic Photosynthetic Organismsmentioning

confidence: 99%

Section: Transgenic Photosynthetic Organismsmentioning

confidence: 99%

See 1 more Smart Citation

Photosymbiosis for Biomedical Applications

Chávez

Moellhoff

Schenck

et al. 2020

Front. Bioeng. Biotechnol.

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“…When a trauma in the skin is created, ROS production is initiated by hypoxia [36]. Furthermore, ROS mediate or recruit immune cells and platelets at the wound site [37], which in turn leads to the release of cytokines, one of which is vascular endothelial growth factor (VEGF), a potent protein produced by cells that is involved in the formation of blood vessels. Therefore, angiogenesis begins immediately after injury.…”

Section: The Impacts Of Gpsma On Skin Tissue Regenerationmentioning

confidence: 99%

The Bioactive Core and Corona Synergism of Quantized Gold Enables Slowed Inflammation and Increased Tissue Regeneration in Wound Hypoxia

Yeh

Chen

Liao

et al. 2020

IJMS

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The progress of wound regeneration relies on inflammation management, while neovascular angiogenesis is a critical aspect of wound healing. In this study, the bioactive core and corona synergism of quantized gold (QG) were developed to simultaneously address these complicated issues, combining the abilities to eliminate endotoxins and provide oxygen. The QG was constructed from ultrasmall nanogold and a loosely packed amine-based corona via a simple process, but it could nonetheless eliminate endotoxins (a vital factor in inflammation also called lipopolysaccharides) and provide oxygen in situ for the remodeling of wound sites. Even while capturing endotoxins through electrostatic interactions, the catalytic active sites inside the nanogold could maintain its surface accessibility to automatically transform the overexpressed hydrogen peroxide in hypoxic wound regions into oxygen. Since the inflammatory stage is an essential stage of wound healing, the provision of endotoxin clearance by the outer organic corona of the QG could slow inflammation in a way that subsequently promoted two other important stages of wound bed healing, namely proliferation and remodeling. Relatedly, the efficacy of two forms of the QG, a liquid form and a dressing form, was demonstrated at wound sites in this study, with both forms promoting the development of granulation, including angiogenesis and collagen deposition. Thus, the simply fabricated dual function nanocomposite presented herein not only offers reduced batch-to-batch variation but also increased options for homecare treatments.

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“…Por otro lado, mediante colaboraciones internacionales (Universidad Técnica de Múnich, y Universidad Ludwig Maximilliam, Alemania), se han estudiado métodos alternativos para la regeneración de la piel, garantizando su apropiada oxigenación y promoviendo su vascularización mediante el uso de microalgas genéticamente modificadas para la liberación de oxígeno y factores de crecimiento humano como VEGF, PDGF y SDF-1 alfa [18], [19].…”

Section: Cultivo De Piel In Vitrounclassified

Cultivo Celular e Ingeniería de Tejidos: Aplicaciones en Biomedicina

Morales-Sánchez

Ulloa-Fernández

Castro-Piedra

et al. 2019

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El Laboratorio de Ingeniería de Tejidos (LAINTEC) del Centro de Investigación en Biotecnología (CIB) de la Escuela de Biología del Instituto Tecnológico de Costa Rica (TEC) nació en el 2005 con el objetivo de desarrollar terapias celulares para aplicaciones en medicina regenerativa. Actualmente, el LAINTEC aloja más de una decena de proyectos multidisciplinarios, realizados en colaboración con otros centros de investigación del TEC y con otras universidades a nivel nacional e internacional. Estas investigaciones se enfocan en el desarrollo y evaluación de implantes, biomateriales y terapias regenerativas de piel, músculo y hueso, incluyendo el aislamiento y caracterización de células madre mesenquimales de tejido adiposo. Además, se han establecido modelos celulares y tisulares para la evaluación del potencial bioactivo de distintos agentes con potenciales aplicaciones en biomedicina.

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Development of photosynthetic sutures for the local delivery of oxygen and recombinant growth factors in wounds

Cited by 62 publications

References 55 publications

Photosymbiosis for Biomedical Applications

Photosymbiosis for Biomedical Applications

The Bioactive Core and Corona Synergism of Quantized Gold Enables Slowed Inflammation and Increased Tissue Regeneration in Wound Hypoxia

Cultivo Celular e Ingeniería de Tejidos: Aplicaciones en Biomedicina

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