Development of PCR assay based on ITS2 rDNA polymorphism for the detection and differentiation of<i>Fusarium sporotrichioides</i>

Kulik, Tomasz; Fordoñski, G.; Pszczółkowska, Agnieszka; Plodzien, K.; Lapinski, M.

doi:10.1016/j.femsle.2004.08.037

Cited by 40 publications

(17 citation statements)

References 15 publications

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“…DNA of the mentioned Fusarium isolate were used in PCR with the F. sporotrichioides-specific primers according to Kulik et al (2004) and the F. langsethiae-specific primers (Wilson et al, 2004). Internal transcribed spacer (ITS) regions of isolate was amplified using universal primers ITS4 and ITS5 (White et al, 1990).…”

Section: Identification Of Fusarium Cf Langsethiaementioning

confidence: 99%

Investigation of stored wheat mycoflora, reporting theFusarium cf.langsethiae in three provinces of Iran during 2007

et al. 2009

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-were the predominant Aspergillus species identified as exogenous mycoflora. Aspergillus flavus (26.6%) was the predominant Aspergillus species identified as endogenous mycoflora. Flotation method with MGA 0.25 recommended for isolating of hyaline fungi from wheat cereals. In this study one isolate from Fusarium species was isolated on the basis of morphology and ribosomal internal transcribed spacer classified as Fusarium langsethiae but on the basis of partial translation elongation factor-1alpha gene grouped with Fusarium sporotrichioides. To our knowledge, this is the first report about F. cf. langsethiae in Iran and Asia.

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Section: Identification Of Fusarium Cf Langsethiaementioning

confidence: 99%

Investigation of stored wheat mycoflora, reporting theFusarium cf.langsethiae in three provinces of Iran during 2007

et al. 2009

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“…However, these primers generated 2 unexpected bands for most strains of F. sporotrichioides and F. langsethiae and many nonspecific products for other species. Kulik et al [2004] developed an assay to detect F. sporotrichioides based on ITS2. The FspITS2K and P28SL primer pair amplified a fragment of 288 bp.…”

Section: Pcr Assaysmentioning

confidence: 99%

A Review of Conventional PCR Assays for the Detection of Selected Phytopathogens of Wheat

Kuzdraliński

Kot²,

Szczerba³

et al. 2017

Microb Physiol

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Infection of phyllosphere (stems, leaves, husks, and grains) by pathogenic fungi reduces the wheat yield and grain quality. Detection of the main wheat pathogenic fungi provides information about species composition and allows effective and targeted plant treatment. Since conventional procedures for the detection of these organisms are unreliable and time consuming, diagnostic DNA-based methods are required. Nucleic acid amplification technologies are independent of the morphological and biochemical characteristics of fungi. Microorganisms do not need to be cultured. Therefore, a number of PCR-based methodologies have been developed for the identification of key pathogenic fungi, such as Fusarium spp., Puccinia spp., Zymoseptoria tritici, Parastagonospora nodorum, Blumeria graminis f. sp. tritici, and Pyrenophora tritici-repentis. This article reviews frequently used DNA regions for fungus identification and discusses already known PCR assays for detection of the aforementioned wheat pathogens. We demonstrate that PCR-based wheat pathogen identification assays require further research. In particular, the number of diagnostic tests for Fusarium graminearum, Puccinia spp., and P. tritici-repentis are insufficient.

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“…The species F. sporotrichioides, of the section Sporotrichiella, is widespread on plants and in the soil throughout the cold and cool regions of the world (Kulik et al 2004;Visconti et al 1985). This species was often isolated from oat panicles and kernels.…”

Section: Introductionmentioning

confidence: 99%

Fusarium Sporotrichioides Sherb. Toxins Evaluated in Cereal Grain with Trichoderma Harzianum

Wiśniewska¹,

Basiński²,

Chełkowski³

et al. 2011

Journal of Plant Protection Research

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Fusarium head blight is one of the most severe diseases of small grain cereals and is caused by several toxigenic Fusarium species. Yield losses and mycotoxin accumulation in grain are caused by the disease. F. sporotrichioides and F. poae produce type A trichothecenes. Saprophyte fungal antagonists, especially Trichoderma harzianum, are effective biocontrol agents against several fungal soil-borne plant pathogens. These fungal antagonists can reduce the production of Fusarium spp. mycotoxins in some crop plants. The aim of this study was to examine the influence of T. harzianum AN4 on the production of type A trichothecenes by F. sporotrichioides in cereals. The accumulation of six trichothecene mycotoxins (scirpentriol, i.e. STO, T-2 tetraol, T-2 triol, HT-2 toxin i.e., HT-2, T-2 toxin i.e., T-2, diacetoxyscirpenol, i.e., DAS) was reduced on average, by over 89% in bioassays of F. sporotrichioides and Trichoderma isolate AN4 on a liquid medium and on solid substrates (seeds of naked and husked oats and wheat). The reduction in our experiments depended on fungal isolate and substrate. From the three isolates of F. sporotrichioides used in the experiments, the highest accumulation of all the metabolites after 21 days by F. sporotrichioides in nearly all substrates, was recorded for strain ZFR 159. On the liquid medium inoculated with F. sporotrichioides ZFR 159, the amount of type A trichothecenes was the lowest (STO, T-2 tetraol, and T-2 triol not detected, HT-2 toxin 0.02 ppm, DAS 0.10 ppm, T-2 toxin 0.99 ppm). The highest total concentration of these toxins was produced by this isolate in husked oat cv. German (180.16 ppm), but in naked oat cv. Akt the toxin concentration was low (27.62 ppm). Trichothecene accumulation by T. harzianum AN4 was reduced the most in oat cv. Akt (98.48%) in the liquid medium (98.22%), while the lowest reduction was in oat cv. German (48.77%). The non-toxigenic T. harzianum AN4 isolate proved to be a useful biocontrol agent against the toxigenic F. sporotrichioides in cereals, significantly reducing the production of six type A trichothecenes. This is the first report on effective biocontrol of F. sporotrichioides in cereals by T. harzianum.

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Development of PCR assay based on ITS2 rDNA polymorphism for the detection and differentiation ofFusarium sporotrichioides

Cited by 40 publications

References 15 publications

Investigation of stored wheat mycoflora, reporting theFusarium cf.langsethiae in three provinces of Iran during 2007

Investigation of stored wheat mycoflora, reporting theFusarium cf.langsethiae in three provinces of Iran during 2007

A Review of Conventional PCR Assays for the Detection of Selected Phytopathogens of Wheat

Fusarium Sporotrichioides Sherb. Toxins Evaluated in Cereal Grain with Trichoderma Harzianum

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