Development of one-step real-time RT-PCR assay for detection and quantitation of peste des petits ruminants virus

Bao, Jingyue; Li, Lin; Wang, Zhiliang; Barrett, Tom; Suo, Longciren; Wen-ji, Zhao; Liu, Yutian; Liu, Chunquan; Li, Jinming

doi:10.1016/j.jviromet.2007.12.003

Cited by 75 publications

(59 citation statements)

References 18 publications

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“…Therefore, specific diagnosis of PPRV infection can be achieved by cDNA hybridization [41,105,127,149], mobility of N protein [40,149], neutralization test [28,116,149] and MAb-based ELISA [81,82,135,136], RT-PCR [7,53] and Real time RT-PCR [12,13,18]. A battery of serological tests and molecular assays are available to detect and identify PPRV antigen/nucleic acid and antibodies.…”

Section: Diagnosismentioning

confidence: 99%

“…Earlier, N gene based one step TaqMan real-time RT-PCR has been developed for detection of PPRV nucleic acid in the clinical samples with high sensitivity [18]. Recently, M gene based two step TaqMan hydrolysis probe [12] and one step real-time RT-PCR based on SYBR Green chemistry [13] have been optimized for specific detection of PPRV in clinical samples.…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

See 1 more Smart Citation

Diagnosis and control of peste des petits ruminants: a comprehensive review

Balamurugan¹,

Hemadri²,

Gajendragad³

et al. 2013

VirusDis.

120

109

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Peste des petits ruminants (PPR) is an acute, highly contagious, world organization for animal health (OIE) notifiable and economically important transboundary viral disease of sheep and goats associated with high morbidity and mortality and caused by PPR virus. PPR is considered as one of the main constraints in augmenting the productivity of small ruminants in developing countries and particularly severely affects poor farmer's economy. The disease is clinically manifested by pyrexia, oculo-nasal discharges, necrotizing and erosive stomatitis, gastroenteritis, diarrhoea and bronchopneumonia. The disease can be diagnosed from its clinical signs, pathological lesions, and specific detection of virus antigen/antibodies/genome in the clinical samples by various serological tests and molecular assays. PPR is the one of the priority animal diseases whose control is considered important for poverty alleviation in enzootic countries. Availability of effective and safe live attenuated cell culture PPR vaccines and diagnostics have boosted the recently launched centrally sponsored control programme in India and also in other countries. This review article primarily focus on the current scenario of PPR diagnosis and its control programme with advancement of research areas that have taken place in the recent years with future perspectives.

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Section: Diagnosismentioning

confidence: 99%

Section: Polymerase Chain Reactionmentioning

confidence: 99%

Diagnosis and control of peste des petits ruminants: a comprehensive review

Balamurugan¹,

Hemadri²,

Gajendragad³

et al. 2013

VirusDis.

120

109

View full text Add to dashboard Cite

show abstract

“…9.4, 9.5). Particularly, for the detection of PPRV genome, the TaqMan technology approach has been used by various authors (Bao et al 2008;Wang et al 2009;Kwiatek et al 2010;Batten et al 2011;Polci et al 2013). …”

Section: Real-time Rt-pcrmentioning

confidence: 99%

Current Advances in Genome Detection of Peste des Petits Ruminants Virus

Couacy‐Hymann

2014

Peste Des Petits Ruminants Virus

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Molecular techniques have given various opportunities to detect the genome of peste des petits ruminants virus (PPRV) at high resolutions, and these powerful methods are very sensitive and specific. In the past, radioisotope-based techniques have been used for diagnostic purposes. However, because of associated hazards these may cause to human and environment, the radioelement hybridization techniques are no more in use. Alternative techniques such as hybridization with the digoxigenin/anti-digoxigenin system have been developed and are being practised. Moreover, genome amplification with different polymerase chain reaction (PCR) chemistries (conventional PCR, real-time PCR, multiplex real-time PCR, LAMP-PCR) has been developed to easily detect genome of PPRV, independent of lineage variations. Prior to these, adequate samples should be taken from sick animals and should be well conserved and transported rapidly to the laboratory for analysis. Despite convincing performance of these new diagnostic methods, currently, it is not possible to directly differentiate lineages of PPRV strains, which are now prevalent without distinct geographical demarcation. Currently, the amplified PCR products are sequenced to determine genetic classification of PPRV lineages and to establish epidemiological links.

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“…It was not until 2008 when Bao and co-workers developed a very sensitive and specific TaqMan based, one-step real-time quantitative reverse transcription PCR (qRT-PCR) for the detection of PPRV in field samples (Bao et al 2008), which proved to be very useful for the analysis of samples collected during PPR epidemic in Tibet in 2007. This assay overwhelmed the existing RT-PCRs for rapid, specific, and sensitive laboratory detection of PPRV in tissue samples from field cases (Bao et al 2008) (Table 6.1). However, the test was not validated on all PPRV lineages, and its performance was not established clearly on field samples.…”

Section: Genome Detection For Ppr Diagnosismentioning

confidence: 99%

Current Advances in Molecular Diagnosis and Vaccines for Peste des Petits Ruminants

Munir

Zohari²,

Berg

2012

Molecular Biology and Pathogenesis of Peste Des Petits Ruminants Virus

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There has been a substantial improvement in the detection of the nucleic acids of Peste des Petits Ruminants Virus (PPRV), and effective vaccines have been developed in recent years. Demonstrations of several real-time PCR assays have provided powerful and novel means of not only detection but also quantification of the nucleic acids of PPRV in several types of clinical samples. Although most of the lineages are continent specific, reports on mixed lineages are emerging, such as in Sudan and Uganda. None of the available assays is devised so far to differentiate all of the lineages. Despite essential advances in the marker vaccines, it is still required to establish reverse genetics systems to rescue recombinant PPRV vaccines, primarily by manipulating the genes of PPRV and insertion of positive or negative markers, which ultimately will lead to the development of companion test. Similarly, swapping different genes will facilitate the establishment of a test that can differentiate vaccinated and infected animals. In this chapter, we discuss available diagnostic tests and potent PPRV vaccines. Furthermore, the current advances in both fields and possibilities to develop next-generation assays and vaccines for PPRV are critically discussed.

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Development of one-step real-time RT-PCR assay for detection and quantitation of peste des petits ruminants virus

Cited by 75 publications

References 18 publications

Diagnosis and control of peste des petits ruminants: a comprehensive review

Diagnosis and control of peste des petits ruminants: a comprehensive review

Current Advances in Genome Detection of Peste des Petits Ruminants Virus

Current Advances in Molecular Diagnosis and Vaccines for Peste des Petits Ruminants

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