Development of novel real-time PCR methodology for quantification of COL11A1 mRNA variants and evaluation in breast cancer tissue specimens

Karaglani, Makrina; Toumpoulis, Ioannis K.; Goutas, Nikolaοs; Poumpouridou, Nikoleta; Vlachodimitropoulos, Dimitrios; Vasilaros, Spyridon; Rizos, Ioannis; Kroupis, Christos

doi:10.1186/s12885-015-1725-8

Cited by 9 publications

(7 citation statements)

References 41 publications

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“… 22 , 23 Type XI collagen involvement has been reported in human diseases whereby type XI collagen is highly expressed in cancers but is almost always absent in non-cancerous tissues. 24 Notably, even in an early stage of cancer, collagen XI protein is expressed at high levels and has been shown to stimulate the stromal proliferative response of tumor-associated fibroblasts and to promote tumor cell metastasis. 25 , 26 Therefore, COL11A1 expression should be investigated for effects on CRC initiation and progression and evaluated for its suitability as a CRC biomarker.…”

Section: Introductionmentioning

confidence: 99%

BGN and COL11A1 Regulatory Network Analysis in Colorectal Cancer (CRC) Reveals That BGN Influences CRC Cell Biological Functions and Interacts with miR-6828-5p

Chen¹,

Qin

Dai³

et al. 2020

CMAR

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Purpose We explored specific expression profiles of BGN and COL11A1 genes and studied their biological functions in CRC using bioinformatics tools. Patients and Methods A total of 68 pairs of cancer and non-cancerous tissues from CRC patients were enrolled in this study. Methods we used in this articles including: qRT-PCR, Western blot analysis, ELISA, GO and KEGG regulatory network analysis, tumor infiltration, luciferase reporter-based protein and etc. Results According to The Cancer Genome Atlas (TCGA) data, BGN and COL11A1 expression levels were significantly higher in CRC patient samples than in samples from healthy controls. Moreover, levels were much higher in late-stage CRC than in early-stage disease, warranting evaluation of these genes as CRC prognostic biomarkers. Subsequently, qRT-PCR, Western blot analysis, and ELISA results obtained from analyses of CRC cells, tissues, and patient sera aligned with TCGA results. GO and KEGG regulatory network analysis revealed BGN- and COL11A1- associated genes that were functionally related to extracellular matrix (ECM) receptor pathway activation, with transcription factor genes RELA and NFKB1 positively associated with BGN expression and CEBPZ and SIRT1 with COL11A1 expression. Meanwhile, BGN and COL11A1 expression were separately and significantly correlated to tumor infiltration by six immune cell types. Additionally, kinase genes PLK1 and LYN appeared to be downstream targets of differentially expressed BGN and COL11A1 , respectively. In addition, the expression of PLK1 mRNA was down-regulated while BGN was down-regulated. Finally, BGN effects on CRC cell proliferation, cycle, apoptosis, invasion, and migration were studied using molecular biological methods, including luciferase reporter-based protein analysis, qRT-PCR, and Western blot results, which revealed that miR-6828-5p may regulate BGN expression. Conclusion We speculate that the use of BGN and COL11A1 as CRC biomarkers would improve CRC staging, while also providing several novel targets for use in the development of more effective CRC treatments.

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Section: Introductionmentioning

confidence: 99%

BGN and COL11A1 Regulatory Network Analysis in Colorectal Cancer (CRC) Reveals That BGN Influences CRC Cell Biological Functions and Interacts with miR-6828-5p

Chen¹,

Qin

Dai³

et al. 2020

CMAR

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“…Collagen was reported to be active in numerous biological processes in cancers [12]. Collagen type XI alpha 1 chain (COL11A1), a subtype of fibrillary type collagen, plays a role in extracellular matrix and tissues and was over-expressed in human tumors [13]. COL11A1 has also been regarded as a promising prognostic biomarker for breast cancer [13].…”

Section: Introductionmentioning

confidence: 99%

“…Collagen type XI alpha 1 chain (COL11A1), a subtype of fibrillary type collagen, plays a role in extracellular matrix and tissues and was over-expressed in human tumors [13]. COL11A1 has also been regarded as a promising prognostic biomarker for breast cancer [13]. On the basis of existing literature data, we carried out studies in an effort to investigate the possible CDX2 may act as a significant cytokine in breast cancer.…”

Section: Introductionmentioning

confidence: 99%

Over-expression of CDX2 alleviates breast cancer by up-regulating microRNA let-7b and inhibiting COL11A1 expression

et al. 2020

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Background: microRNA Let-7 serves as a tumor suppressor by targeting various oncogenic pathways in cancer cells. However, the underlying mechanism of its involvement in breast cancer remains largely unknown. With our research, our endeavor is to explore the role of the CDX2/let-7b/COL11A1 axis in breast cancer cell activities. Methods: Tumor tissues and adjacent normal tissues were collected from 86 patients with breast cancer. Human breast cancer epithelial cell line MCF-7 was treated with over-expressed CDX2, let-7b mimic, shRNA against COL11A1 and their negative controls. The expression of CDX2, let-7b, and COL11A1 in the tissues and cells was determined by RT-qPCR. Interactions among CDX2, let-7b, and COL11A1 were detected by ChIP and dual-luciferase reporter assay, respectively. After different transfections, cell invasion, migration, and proliferation abilities were determined by Transwell and EdU assays. Lastly, tumor xenografts in nude mice were established and hematoxylin and eosin staining was performed to assess the tumor growth and lymph node metastasis. Results: CDX2 and let-7b were poorly expressed in breast cancer cells and tissues. CDX2 bound to let-7b and promoted the expression of let-7b, which contrarily inhibited the expression of COL11A1. Cancer cell proliferation, invasion, migration, and metastasis were stimulated when CDX2 and let-7b were depleted or COL11A1 was overexpressed. Xenograft tumors growth and metastasis were in accordance with the results of cellular experiments. Conclusion: In agreement with these observations, we could reach a conclusion that CDX2 could promote let-7b expression, which may exert an inhibitory effect on the proliferation, migration, and metastasis of breast cancer cells via repressing the expression of COL11A1, providing a novel therapeutic strategy for the treatment of metastatic breast cancer.

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Section: Introductionmentioning

confidence: 99%

“…Collagen Type XI Alpha 1 Chain (COL11A1) is a subtype of fibrillar collagen, which was proved to be related to cancer cell proliferation, migration, and tumorigenesis [ 16 ]. COL11A1 is also thought to be a potential prognostic biomarker for breast cancer [ 16 ]. Moreover, previous studies revealed that COL11A1 strengthens cancer cell invasion through cooperating with invasion-sensing biomarkers [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

COL11A1 is Downregulated by miR-339-5p and Promotes Colon Carcinoma Progression

Liu

Meng

2022

Canadian Journal of Gastroenterology and Hepatology

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The roles of COL11A1 in cancer have been increasingly considered, but the understandings of the effects of COL11A1 on colon carcinoma progress are much limited yet. qRT-PCR and Western blot were utilized to evaluate COL11A1 expression at mRNA and protein levels, respectively, in colon carcinoma cell lines. Afterward, the tumorigenesis biological effects of COL11A1 were examined by CCK-8, colony formation, Transwell, and wound healing methods. Moreover, upstream miRNAs containing the binding sites with COL11A1 were predicted by the bioinformatics methods. The interplay between COL11A1 and miR-339-5p was identified by a dual-luciferase assay. COL11A1 expression was prominently upregulated in colon carcinoma cell lines relative to that in normal human colon mucosal epithelial cell lines, and it was related to tumor stages. The outcomes of in-vitro experiments suggested that interfering with COL11A1 remarkably repressed the malignant behaviors of SW480 and SW620 cells. MiR-339-5p was markedly lowly expressed in colon carcinoma cell lines. Furthermore, miR-339-5p directly targeted and negatively regulated COL11A1 expression. COL11A1 upregulation promoted colon carcinoma cell functions, while overexpressing miR-339-5p evidently attenuated the promotion. These results proved the modulation of the miR-339-5p/COL11A1 axis in colon carcinoma cells, and miR-339-5p repressed colon carcinoma progression via COL11A1 downregulation. These results offer new underlying targets for the accurate therapy of colon carcinoma patients.

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Development of novel real-time PCR methodology for quantification of COL11A1 mRNA variants and evaluation in breast cancer tissue specimens

Cited by 9 publications

References 41 publications

BGN and COL11A1 Regulatory Network Analysis in Colorectal Cancer (CRC) Reveals That BGN Influences CRC Cell Biological Functions and Interacts with miR-6828-5p

BGN and COL11A1 Regulatory Network Analysis in Colorectal Cancer (CRC) Reveals That BGN Influences CRC Cell Biological Functions and Interacts with miR-6828-5p

Over-expression of CDX2 alleviates breast cancer by up-regulating microRNA let-7b and inhibiting COL11A1 expression

COL11A1 is Downregulated by miR-339-5p and Promotes Colon Carcinoma Progression

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