COL11A1 is Downregulated by miR-339-5p and Promotes Colon Carcinoma Progression

Abstract

The roles of COL11A1 in cancer have been increasingly considered, but the understandings of the effects of COL11A1 on colon carcinoma progress are much limited yet. qRT-PCR and Western blot were utilized to evaluate COL11A1 expression at mRNA and protein levels, respectively, in colon carcinoma cell lines. Afterward, the tumorigenesis biological effects of COL11A1 were examined by CCK-8, colony formation, Transwell, and wound healing methods. Moreover, upstream miRNAs containing the binding sites with COL11A1 were predicted by the bioinformatics methods. The interplay between COL11A1 and miR-339-5p was identified by a dual-luciferase assay. COL11A1 expression was prominently upregulated in colon carcinoma cell lines relative to that in normal human colon mucosal epithelial cell lines, and it was related to tumor stages. The outcomes of in-vitro experiments suggested that interfering with COL11A1 remarkably repressed the malignant behaviors of SW480 and SW620 cells. MiR-339-5p was markedly lowly expressed in colon carcinoma cell lines. Furthermore, miR-339-5p directly targeted and negatively regulated COL11A1 expression. COL11A1 upregulation promoted colon carcinoma cell functions, while overexpressing miR-339-5p evidently attenuated the promotion. These results proved the modulation of the miR-339-5p/COL11A1 axis in colon carcinoma cells, and miR-339-5p repressed colon carcinoma progression via COL11A1 downregulation. These results offer new underlying targets for the accurate therapy of colon carcinoma patients.

Characteristics of endoplasmic reticulum stress in colorectal cancer for predicting prognosis and developing treatment options

“… 45 , 52 In CRC, high expression of COL11A1 was not only positively correlated with the tumor stage but also with the malignant behavior of CRC, and miR‐339‐5p could down‐regulate COL11A1 expression and inhibit colorectal progression. 53 We have verified the mRNA levels of these genes in CRC specimens by qRT‐PCR; however, further basic studies are needed to elucidate their functions in CRC. In CRC, the discovery of ERS‐related prognostic key genes has provided new clues to explore the molecular pathways of ERS and new ideas for the study of targeted therapies in CRC.…”

“…COL11A1 is an isoform of fibrillar collagen that is involved in the proliferation, migration and apoptosis of cancer cells 45,52 . In CRC, high expression of COL11A1 was not only positively correlated with the tumor stage but also with the malignant behavior of CRC, and miR‐339‐5p could down‐regulate COL11A1 expression and inhibit colorectal progression 53 . We have verified the mRNA levels of these genes in CRC specimens by qRT‐PCR; however, further basic studies are needed to elucidate their functions in CRC.…”

Targets in the Tumour Matrisome to Promote Cancer Therapy Response

“…Col11a1+ CAFs, which co-express LRRC15, are also associated with tumour progression in scRNA-seq and tissue-staining studies [106,107]. Other studies have identified COL11A1 as a functional driver of cancer progression and prognostic biomarker [108][109][110]. A similar body of the literature is emerging supporting pro-tumourigenic functions for COLVI in breast, ovarian [111], pancreatic [112], lung [113], and brain [114] cancers.…”

Circ_0091822 Contributes to the Proliferation, Invasion, and Migration of Vascular Smooth Muscle Cells Under Oxidized Low-Density Lipoprotein Treatment

“…Studies had revealed that miR-339-5p might regulate the progression of age-related neurodegeneration, which overexpression could promote spinal morphogenesis and calcium homeostasis (25). MiR-339-5p plays an antitumor role in many types of cancer by inhibiting cell proliferation, invasion, and migration, such as colon carcinoma (26) and prostate cancer (27). Furthermore, miR-339-5p abnormal expression also mediated the chemo-resistance development of laryngeal carcinoma (28).…”