Development of new generation of vaccines for Brucella abortus

Gheibi, Azam; Khanahmad, Hossein; Kashfi, Khosrow; Sarmadi, Mahdieh; Khorramizadeh, Mohammad Reza

doi:10.1016/j.heliyon.2018.e01079

Cited by 32 publications

(17 citation statements)

References 118 publications

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“…There are genes associated with virulence or organism survival; research into these genes will aid in the development of safe and protective vaccines, such as live but attenuated vaccines, which provide significant amounts of protection [ 70 ]. Purine biosynthesis pathway genes ferro chelatase hem H mutant, a gene required to transport lipid A 363 fatty acid, the phosphoglycerate kinase-encoding gene, LPS biosynthesis pathway genes, and 364 Type IV secretion Vir B genes are just a few examples of vaccines developed using deletions in B. abortus virulence genes that result in major attenuation [ 71 , 72 , 73 , 74 , 75 , 76 ].…”

Section: Discussionmentioning

confidence: 99%

Antibiogram Screening and Detection of Virulence-Associated Genes in Brucella Species Acquired from Cattle in South Africa’s Eastern Cape Province

Manafe

Bhembe-Magadaza

Green

2022

IJERPH

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Brucellosis is a widespread zoonotic illness, and it poses serious public health and economic risks. The purpose of this investigation is to look at the antimicrobial susceptibility of unpasteurized milk, blood, and lymph node specimens from cattle, goats, and sheep, as well as to identify virulence-associated genes. In this investigation, a total of 123 isolates were examined. The activity of 15 antimicrobials against Brucella pathogens were assessed using the Kirby–Bauer disk diffusion technique. Nine virulence factors were detected with polymerase chain reaction analysis. Five antibiotics were 100% effective against Brucella isolates. A high level of resistance (100%) was documented with streptomycin, penicillin, and seven more antibiotics. Doxycycline resistance was found in 12% of goat isolates, and tetracycline resistance was found in 21% and 44% of goat and sheep isolates, respectively. Multiple antibiotic resistance (MAR) index >0.2 was found in 38.2% (47/123) of Brucella isolates. VecC and BetB, two B. abortus genes, were confirmed to be comparable. The findings of this study suggests that Brucella spp. are reservoirs of antibiotic resistance in the Eastern Cape Province. As such, they represent a potential pool of antibiotic genes that might be transferred to other pathogens in the community, and thus continue to pose a healthcare hazard.

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Section: Discussionmentioning

confidence: 99%

Antibiogram Screening and Detection of Virulence-Associated Genes in Brucella Species Acquired from Cattle in South Africa’s Eastern Cape Province

Manafe

Bhembe-Magadaza

Green

2022

IJERPH

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“…Although S19∆per has the added advantage of the safety parameters, to be considered as a potential vaccine candidate, it needs to commensurate with the inherent ability of conferring protection against infection. The LPS deficient mutants have been demonstrated to be highly attenuated in a number of studies, but in many instances, they failed to induce protective immune responses [47,48].…”

Section: Discussionmentioning

confidence: 99%

Immunization with Brucella abortus S19Δper Conferred Protection in Water Buffaloes against Virulent Challenge with B. abortus Strain S544

et al. 2021

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Vaccination of cattle and buffaloes with Brucella abortus strain 19 has been the mainstay for control of bovine brucellosis. However, vaccination with S19 suffers major drawbacks in terms of its safety and interference with serodiagnosis of clinical infection. Brucella abortus S19∆per, a perosamine synthetase wbkB gene deletion mutant, overcomes the drawbacks of the S19 vaccine strain. The present study aimed to evaluate the potential of Brucella abortus S19Δper vaccine candidate in the natural host, buffaloes. Safety of S19∆per, for animals use, was assessed in guinea pigs. Protective efficacy of vaccine was assessed in buffaloes by immunizing with normal dose (4 × 1010 colony forming units (CFU)/animal) and reduced dose (2 × 109 CFU/animal) of S19Δper and challenged with virulent strain of B. abortus S544 on 300 days post immunization. Bacterial persistency of S19∆per was assessed in buffalo calves after 42 days of inoculation. Different serological, biochemical and pathological studies were performed to evaluate the S19∆per vaccine. The S19Δper immunized animals showed significantly low levels of anti-lipopolysaccharides (LPS) antibodies. All the immunized animals were protected against challenge infection with B. abortus S544. Sera from the majority of S19Δper immunized buffalo calves showed moderate to weak agglutination to RBPT antigen and thereby, could apparently be differentiated from S19 vaccinated and clinically-infected animals. The S19Δper was more sensitive to buffalo serum complement mediated lysis than its parent strain, S19. Animals culled at 6-weeks-post vaccination showed no gross lesions in organs and there was comparatively lower burden of infection in the lymph nodes of S19Δper immunized animals. With attributes of higher safety, strong protective efficacy and potential of differentiating infected from vaccinated animals (DIVA), S19Δper would be a prospective alternate to conventional S19 vaccines for control of bovine brucellosis as proven in buffaloes.

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“…B. abortus strains 19 and RB51 are very efficient and common vaccines being used against bovine brucellosis. B. abortus S19 was a result of natural attenuation lacking 720-bp region in the erythritol catabolic genes (Sangari et al 1994 ; reviewed in Gheibi et al 2018 ). The strain RB51 vaccine does not interfere with serodiagnostic results unlike strain 19 vaccine (Moriyón et al 2004 ).…”

Section: Vaccinationmentioning

confidence: 99%

Bovine brucellosis – a comprehensive review

Khurana

Sehrawat

Tiwari

et al. 2021

Veterinary Quarterly

138

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Brucellosis is a zoonotic disease of great animal welfare and economic implications worldwide known since ancient times. The emergence of brucellosis in new areas as well as transmission of brucellosis from wild and domestic animals is of great significance in terms of new epidemiological dimensions. Brucellosis poses a major public health threat by the consumption of non-pasteurized milk and milk products produced by unhygienic dairy farms in endemic areas. Regular and meticulous surveillance is essentially required to determine the true picture of brucellosis especially in areas with continuous high prevalence. Additionally, international migration of humans, animals and trade of animal products has created a challenge for disease spread and diagnosis in non-endemic areas. Isolation and identification remain the gold standard test, which requires expertise. The advancement in diagnostic strategies coupled with screening of newly introduced animals is warranted to control the disease. Of note, the diagnostic value of miRNAs for appropriate detection of B. abortus infection has been shown. The most widely used vaccine strains to protect against Brucella infection and related abortions in cattle are strain 19 and RB51. Moreover, it is very important to note that no vaccine, which is highly protective, safe and effective is available either for bovines or human beings. Research results encourage the use of bacteriophage lysates in treatment of bovine brucellosis. One Health approach can aid in control of this disease, both in animals and man.

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Development of new generation of vaccines for Brucella abortus

Cited by 32 publications

References 118 publications

Antibiogram Screening and Detection of Virulence-Associated Genes in Brucella Species Acquired from Cattle in South Africa’s Eastern Cape Province

Antibiogram Screening and Detection of Virulence-Associated Genes in Brucella Species Acquired from Cattle in South Africa’s Eastern Cape Province

Immunization with Brucella abortus S19Δper Conferred Protection in Water Buffaloes against Virulent Challenge with B. abortus Strain S544

Bovine brucellosis – a comprehensive review

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