Development of Neisseria gonorrhoeae in vitro susceptibility test methods for cefixime including quality control guidelines

Jones, Ronald N.; Gerlach, E.; Koontz, Franklin P.; Murray, Patrick R.; Pfaller, Michael A.; Washington, John A.; Erwin, Meridith E.; Knapp, C C

doi:10.1016/0732-8893(91)90065-n

Cited by 8 publications

(7 citation statements)

References 13 publications

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“…In several studies, other 1-lactams such as cefmetazole (26), cefotetan (59), and cefuroxime (40) showed diminished in vitro potency for CMRNG compared with penicillin-susceptible strains. In some cases, there was a weaker association between the MIC of penicillin and those of spectinomycin and tetracycline (61).…”

Section: Benefits and Problems Of Astmentioning

confidence: 99%

“…There is ample evidence that gonococcal AST has been and continues to be consistent and reliable despite minor variations in recommended test conditions. With a commercial, prepared agar medium for disk diffusion testing available and with the careful determination of quality control parameters assembled by large multicenter studies (57,58,105), it will be interesting to see whether clinical laboratories will rise to the challenge and begin to determine more antimicrobial susceptibilities for their isolates of N. gonorrhoeae.…”

Section: Cephalosporinsmentioning

confidence: 99%

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Antimicrobial susceptibility testing of Neisseria gonorrhoeae and implications for epidemiology and therapy

Fekete

1993

Clin Microbiol Rev

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Antimicrobial susceptibility testing (AST) of Neisseria gonorrhoeae has been under development since the early days of antimicrobial agents. However, it is rarely applied to clinical isolates today. The history of the various in vitro tests to determine the susceptibility of N. gonorrhoeae to antibiotics is rich with evidence that these results predict response to therapy for almost all agents tested. Further, AST is a useful and important aspect of strain characterization and disease epidemiology in conjunction with the more specific but laborious techniques of auxotyping, serotyping, and plasmid analysis. Current technology has overcome many of the objections to AST for N. gonorrhoeae with standardization of test media and the development of an accurate disk diffusion AST method that is suited to most clinical laboratories regardless of volume or level of technical expertise. Ironically, the very low level of resistance to the current primary treatment strategy in the United States, ceftriaxone or another potent cephalosporin, makes the use of AST somewhat superfluous.

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Section: Benefits and Problems Of Astmentioning

confidence: 99%

Section: Cephalosporinsmentioning

confidence: 99%

Antimicrobial susceptibility testing of Neisseria gonorrhoeae and implications for epidemiology and therapy

Fekete

1993

Clin Microbiol Rev

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“…The Working Group developed disk diffusion and agar dilution procedures, interpretive susceptibility breakpoints, and guidelines for laboratory control strains (N. gonorrhoeae ATCC 49226, N. gonorrhoeae WHO V, and Staphylococcus aureus ATCC 25923) initially for penicillin, spectinomycin, tetracycline, and ceftriaxone (12). Subsequent criteria have been published and accepted by the NCCLS for cefotaxime, ceftazidime, cefuroxime, cefoxitin, and cefixime (11,13). Table 1 summarizes the mean MICs and the MIC ranges for each of the tested drugs for both the SEA and the SD strains.…”

Section: Resultsmentioning

confidence: 99%

“…Susceptibility breakpoints were the only categories proposed for ceftriaxone and cefotaxime by Jones et al (12,13) because of the absence of resistant strains. The susceptibility breakpoints (MIC [zone diameter]) for ceftriaxone and cefotaxime were set at .0.25 ,ug/ml (>35 mm) and c0.5 ug/ml (.31 mm), respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Alternative methods, such as agar dilution antimicrobial testing (17), are generally laborious and not practical for routine use. The development of a standardized agar diffusion method for these tests is more practical, reliable, and reproducible for clinical microbiology laboratories (11,13). The purpose of this study was to evaluate the National Committee for Clinical Laboratory Standards * Corresponding author.…”

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confidence: 99%

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Evaluation of the standardized disk diffusion and agar dilution antibiotic susceptibility test methods by using strains of Neisseria gonorrhoeae from the United States and Southeast Asia

et al. 1992

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Presently, most Neisseria gonorrhoeae testing is based on f-lactamase tests and agar dilution with common therapeutic agents. The National Committee for Clinical Laboratory Standards (NCCLS) recently described a disk diffusion test that produced results similar to the reference agar dilution method for the antibiotic susceptibility of N. gonorrhoeae. We obtained 71 gonococcal isolates from active-duty males aboard a United States Navy vessel while deployed in the Western Pacific during 1989. In addition, 47 isolates ofN. gonorrhoeae were obtained from sexually transmitted disease clinics within the branch clinic operations of the Naval Hospital, San Diego (SD), and tested. Antibiotic susceptibility tests by using the NCCLS agar dilution and disk diffusion techniques were compared. Among the Southeast Asia (SEA) isolates, 47% were 1i-lactamase producers compared with 10.5% of the SD isolates. The mean MICs (SEA/SD) in micrograms per milliliter for both groups were as follows:

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Recent trends in the college of American pathologists proficiency results for antimicrobial susceptibility testing: Preparing for CLIA '88

Jones

1992

Clinical Microbiology Newsletter

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Development of Neisseria gonorrhoeae in vitro susceptibility test methods for cefixime including quality control guidelines

Cited by 8 publications

References 13 publications

Antimicrobial susceptibility testing of Neisseria gonorrhoeae and implications for epidemiology and therapy

Antimicrobial susceptibility testing of Neisseria gonorrhoeae and implications for epidemiology and therapy

Evaluation of the standardized disk diffusion and agar dilution antibiotic susceptibility test methods by using strains of Neisseria gonorrhoeae from the United States and Southeast Asia

Recent trends in the college of American pathologists proficiency results for antimicrobial susceptibility testing: Preparing for CLIA '88

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