Development of near-isogenic lines for a major QTL on 3BL conferring Fusarium crown rot resistance in hexaploid wheat

Ma, Jun; Yan, Guijun; Liu, Chunji

doi:10.1007/s10681-011-0414-1

Cited by 57 publications

(37 citation statements)

References 20 publications

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“…The use of the same source of resistance could be responsible for the detection of the strong correlations between FCR severity and plant height in this study from both of the populations assessed. These results reaffirm the need of determining whether FCR resistance and plant height at this locus were conditioned by the same gene(s), which may need the development and exploitation of some specific genetic stocks such as near isogenic lines (Ma et al 2012) and NIL-derived populations.…”

Section: Discussionsupporting

confidence: 58%

Enhancing Fusarium crown rot resistance by pyramiding large-effect QTL in barley

et al. 2015

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Fusarium crown rot (FCR) is a serious disease in wheat and barley in semiarid regions worldwide. The feasibility of enhancing FCR resistance by gene pyramiding was investigated by generating and assessing two populations segregating for three large-effect QTL located on the long arms of chromosomes 1H, 3H and 4H, respectively. Significant effects were detected for each of the three QTL in both of the populations assessed. Lines with any combination of two resistant alleles from the three QTL gave, on average, significantly better resistance than those with a single resistant allele only, and lines with resistant alleles from all three of the QTL gave the least FCR symptom. However, wide variations in FCR severity were detected for lines belonging to each of the groups with different numbers of resistant alleles. Significant effects of plant height on FCR were detected in both populations, and a significant association between heading date and FCR severity was detected in one of the two populations assessed. Results from the current study also showed that the effects of a given allele of resistance decreased with the increase in the number of resistant alleles an individual group possessed. Overall, results from this study demonstrate that gene pyramiding can be an effective approach in improving FCR resistance and those lines with resistant alleles from all of the three QTL could be valuable for breeding programs.

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Section: Discussionsupporting

confidence: 58%

Enhancing Fusarium crown rot resistance by pyramiding large-effect QTL in barley

et al. 2015

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“…Qcrs.cpi.3B , one of the strongest FCR QTLs, is derived from Triticum spelta , a wild relative of hexaploid or bread wheat (Ma et al ., ). Qcrs.cpi.3B is located on the long arm of chromosome 3B (Ma et al ., ), and resistant and susceptible near‐isogenic lines (NILs) with this locus have been generated to facilitate fine‐mapping and cloning of the gene underlying this QTL (Ma et al ., ; Zheng et al ., ).…”

Section: Breeding For Fcr Resistance and Tolerancementioning

confidence: 99%

Fusarium crown rot caused by Fusarium pseudograminearum in cereal crops: recent progress and future prospects

Kazan

Gardiner

2018

Molecular Plant Pathology

201

158

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Diseases caused by Fusarium pathogens inflict major yield and quality losses on many economically important plant species worldwide, including cereals. Fusarium crown rot (FCR), caused by Fusarium pseudograminearum, is a cereal disease that occurs in many arid and semi-arid cropping regions of the world. In recent years, this disease has become more prevalent, in part as a result of the adoption of moisture-preserving cultural practices, such as minimum tillage and stubble retention. In this pathogen profile, we present a brief overview of recent research efforts that have not only advanced our understanding of the interactions between F. pseudograminearum and cereal hosts, but have also provided new disease management options. For instance, significant progress has been made in the genetic characterization of pathogen populations, the development of new tools for disease prediction, and the identification and pyramiding of loci that confer quantitative resistance to FCR in wheat and barley. In addition, transcriptome analyses have revealed new insights into the processes involved in host defence. Significant progress has also been made in understanding the mechanistic details of the F. pseudograminearum infection process. The sequencing and comparative analyses of the F. pseudograminearum genome have revealed novel virulence factors, possibly acquired through horizontal gene transfer. In addition, a conserved pathogen gene cluster involved in the degradation of wheat defence compounds has been identified, and a role for the trichothecene toxin deoxynivalenol (DON) in pathogen virulence has been reported. Overall, a better understanding of cereal host-F. pseudograminearum interactions will lead to the development of new control options for this increasingly important disease problem. Taxonomy: Fusarium pseudograminearum O'Donnell & Aoki; Kingdom Fungi; Phylum Ascomycota; Subphylum Pezizomycotina; Class Sordariomycetes; Subclass Hypocreomycetidae; Order Hypocreales; Family Nectriaceae; Genus Fusarium. Disease symptoms: Fusarium crown rot caused by F. pseudograminearum is also known as crown rot, foot rot and root rot. Infected seedlings can die before or after emergence. If infected seedlings survive, typical disease symptoms are browning of the coleoptile, subcrown internode, lower leaf sheaths and adjacent stems and nodal tissues; this browning can become evident within a few weeks after planting or throughout plant development. Infected plants may develop white heads with no or shrivelled grains. Disease symptoms are exacerbated under water limitation. Identification and detection: Fusarium pseudograminearum macroconidia usually contain three to five septa (22-60.5 × 2.5-5.5 μm). On potato dextrose agar (PDA), aerial mycelia appear floccose and reddish white, with red or reddish-brown reverse pigmentation. Diagnostic polymerase chain reaction (PCR) tests based on the amplification of the gene encoding translation elongation factor-1a (TEF-1a) have been developed for molecular identification. Host range: All maj...

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“…Thus, a very important step is to validate QTL effects before further research is pursued into QTL cloning, genomics studies, or Marker-Assisted Selection (MAS) to introgress QTL into new genetic backgrounds. NILs have been used advantageously to identify and validate QTL, as well as to produce fine maps, especially in tomato (Brouwer and St Clair 2004), wheat (Ma et al 2011), maize (Koester et al 1993), rice (Yu et al 1991), barley (Schmalenbach et al 2008), chickpea (Castro et al 2010) and rapeseed (Delourme et al 2008). The goal of MAB is to incorporate a major gene or QTL from an agronomically inferior source (the donor parent) into an elite cultivar or breeding line (the recurrent or recipient parent).…”

Section: Introductionmentioning

confidence: 99%

Validation of QTL for resistance to Aphanomyces euteiches in different pea genetic backgrounds using near-isogenic lines

et al. 2015

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Marker-assisted backcrossing was used to generate pea NILs carrying individual or combined resistance alleles at main Aphanomyces resistance QTL. The effects of several QTL were successfully validated depending on genetic backgrounds. Quantitative trait loci (QTL) validation is an important and often overlooked step before subsequent research in QTL cloning or marker-assisted breeding for disease resistance in plants. Validation of QTL controlling partial resistance to Aphanomyces root rot, one of the most damaging diseases of pea worldwide, is of major interest for the future development of resistant varieties. The aim of this study was to validate, in different genetic backgrounds, the effects of various resistance alleles at seven main resistance QTL recently identified. Five backcross-assisted selection programs were developed. In each, resistance alleles at one to three of the seven main Aphanomyces resistance QTL were transferred into three genetic backgrounds, including two agronomically important spring (Eden) and winter (Isard) pea cultivars. The subsequent near-isogenic lines (NILs) were evaluated for resistance to two reference strains of the main A. euteiches pathotypes under controlled conditions. The NILs carrying resistance alleles at the major-effect QTL Ae-Ps4.5 and Ae-Ps7.6, either individually or in combination with resistance alleles at other QTL, showed significantly reduced disease severity compared to NILs without resistance alleles. Resistance alleles at some minor-effect QTL, especially Ae-Ps2.2 and Ae-Ps5.1, were also validated for their individual or combined effects on resistance. QTL × genetic background interactions were observed, mainly for QTL Ae-Ps7.6, the effect of which increased in the winter cultivar Isard. The pea NILs are a novel and valuable resource for further understanding the mechanisms underlying QTL and their integration in breeding programs.

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Development of near-isogenic lines for a major QTL on 3BL conferring Fusarium crown rot resistance in hexaploid wheat

Cited by 57 publications

References 20 publications

Enhancing Fusarium crown rot resistance by pyramiding large-effect QTL in barley

Enhancing Fusarium crown rot resistance by pyramiding large-effect QTL in barley

Fusarium crown rot caused by Fusarium pseudograminearum in cereal crops: recent progress and future prospects

Validation of QTL for resistance to Aphanomyces euteiches in different pea genetic backgrounds using near-isogenic lines

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