Development of nanostructured biomedical micro-drug testing device based on in situ cellular activity monitoring

Prasad, Shalini; Quijano, Jorge E.

doi:10.1016/j.bios.2005.05.005

Cited by 44 publications

(11 citation statements)

References 44 publications

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“…Optical and spectroscopic methods such as spectrophotometric methods [2], fluorescence microscopy [3] and confocal microscopy [4] can be utilized for the analysis of living cells, but have critical disadvantages due to the presence of exogenous fluorophores, which results in their providing limited information specific to only a small range of subcellular components. Non-optical methods such as cyclic voltammetry (CV) or differential pulsed voltammetry (DPV) have also been employed to analyze the cellular behavior without fluorescence dyes [5], [6]; however, the voltammetric signals achieved using electrochemical tools only represent cell viability, which is insufficient for intensive cell-based research.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Intracellular State Based on Controlled 3D Nanostructures Mediated Surface Enhanced Raman Scattering

El‐Said

Kim

et al. 2011

PLoS ONE

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Near-infrared surface-enhanced Raman spectroscopy (SERS) is a powerful technique for analyzing the chemical composition within a single living cell at unprecedented resolution. However, current SERS methods employing uncontrollable colloidal metal particles or non-uniformly distributed metal particles on a substrate as SERS-active sites show relatively low reliability and reproducibility. Here, we report a highly-ordered SERS-active surface that is provided by a gold nano-dots array based on thermal evaporation of gold onto an ITO surface through a nanoporous alumina mask. This new combined technique showed a broader distribution of hot spots and a higher signal-to-noise ratio than current SERS techniques due to the highly reproducible and uniform geometrical structures over a large area. This SERS-active surface was applied as cell culture system to study living cells in situ within their culture environment without any external preparation processes. We applied this newly developed method to cell-based research to differentiate cell lines, cells at different cell cycle stages, and live/dead cells. The enhanced Raman signals achieved from each cell, which represent the changes in biochemical compositions, enabled differentiation of each state and the conditions of the cells. This SERS technique employing a tightly controlled nanostructure array can potentially be applied to single cell analysis, early cancer diagnosis and cell physiology research.

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Section: Introductionmentioning

confidence: 99%

Analysis of Intracellular State Based on Controlled 3D Nanostructures Mediated Surface Enhanced Raman Scattering

El‐Said

Kim

et al. 2011

PLoS ONE

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“…[16] Nanoporous AAO substrates have also been used as a cellular interface for primary human osteoblast-like cells [17] and nerve cells, [18] and have been embedded in a silicon-based microfluidic system to generate a drug-testing platform. [19] In this study, we fabricated four different types of AAO-based nanotopographical substrates, and investigated how various AAO nanostructures affected the development of primary hippocampal neurons. In particular, we focused on neurite development during the first two days: cultured neurons were categorized into three developmental stages, and their growth was analyzed by neurite-length measurement.…”

mentioning

confidence: 99%

Pitch‐Dependent Acceleration of Neurite Outgrowth on Nanostructured Anodized Aluminum Oxide Substrates

Cho

Kang

et al. 2010

Angew Chem Int Ed

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“…Nanopatterns are used basic neurobiology in tissue-engineered scaffolds [25-27], nerve prostheses [28], and neurobiosensors [13,29]. The current study provides further evidence that nanotopography regulates cell-cell interactions and communication by controlling the cell growth and gap junction proteins.…”

Section: Resultsmentioning

confidence: 57%

Topographical control of cell-cell interaction in C6 glioma by nanodot arrays

2014

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Nanotopography modulates the physiological behavior of cells and cell-cell interactions, but the manner of communication remains unclear. Cell networking (syncytium) of astroglia provides the optimal microenvironment for communication of the nervous system. C6 glioma cells were seeded on nanodot arrays with dot diameters ranging from 10 to 200 nm. Cell viability, morphology, cytoskeleton, and adhesion showed optimal cell growth on 50-nm nanodots if sufficient incubation was allowed. In particular, the astrocytic syncytium level maximized at 50 nm. The gap junction protein Cx43 showed size-dependent and time-dependent transport from the nucleus to the cell membrane. The transport efficiency was greatly enhanced by incubation on 50-nm nanodots. In summary, nanotopography is capable of modulating cell behavior and influencing the cell-cell interactions of astrocytes. By fine-tuning the nanoenvironment, it may be possible to regulate cell-cell communications and optimize the biocompatibility of neural implants.

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Development of nanostructured biomedical micro-drug testing device based on in situ cellular activity monitoring

Cited by 44 publications

References 44 publications

Analysis of Intracellular State Based on Controlled 3D Nanostructures Mediated Surface Enhanced Raman Scattering

Analysis of Intracellular State Based on Controlled 3D Nanostructures Mediated Surface Enhanced Raman Scattering

Pitch‐Dependent Acceleration of Neurite Outgrowth on Nanostructured Anodized Aluminum Oxide Substrates

Topographical control of cell-cell interaction in C6 glioma by nanodot arrays

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