Development of N-ferrocenyl(benzoyl)amino-acid esters stationary phase for high performance liquid chromatography

Abstract

A new stationary phase for high-performance liquid chromatography was prepared by covalently bonding N-ferrocenyl(benzoyl)amino-acid esters (L(1)) onto silica gel using 3-aminopropyltriethoxysilane as coupling reagent. The structure of new material was characterized by infrared spectroscopy, elemental analysis and thermogravimetric analysis. The chromatographic behavior of the phase was illustrated in reversed-phase (RP) and normal-phase (NP) modes using polycyclic aromatic hydrocarbons (PAHs), aromatics positional isomers, amines, 5-nitroimidazoles, organophosphorus pesticides and phenols as probes. Multiple mechanisms including hydrophobic, hydrogen bonding, π-π, dipole-dipole, charge-transfer and acid-base equilibrium interactions are involved. Thus, multi-interaction mechanisms and mixed-mode separation of the new phase can very likely guarantee its excellent chromatographic performance for the analysis of complex samples. The L(1) AminoSil column was successfully employed for the analysis of organophosphorus pesticides in vegetable.

Nanogold hybrid silica gel and its 1-octadecanethiol self-assembled modified composite as a stationary phase for liquid chromatography

“…9 Thus, various modified chromatographic stationary phases based on porous silica have been investigated quantitatively, especially their chemical organic bonding. 5,[10][11][12][13] However, chemical modification always accompanies volatile and toxic substances, such as toluene, 14,15 methanol, 10,16 and chloroform. 17,18 In addition, organic compounds that bond to the stationary phase can be easily sloughed when rushed for a long time.…”

Determination of trace sulfonamides in foodstuffs by HPLC using a novel mixed-mode functionalized ferrocene sorbent for solid-phase extraction cleanup

“…Our group has been committed to the research of ferrocene stationary phases and several novel multi-interaction and mixed-mode stationary phases based on ferrocene complex modied silica gels have been synthesized in our laboratory. 32,33 It is noteworthy that aromatic derivatives, which own polar groups such as carbonyl, NH 2 , OH and NO 2 , might tune the separation selectivity and tend to give better results for ferrocene-bonded phases by contrast. Recently, we prepared a novel functionalized ferrocene modied silica (NFcS) (Fig.…”