Development of multifunctional 3D printed bioscaffolds from polysaccharides and NiCu nanoparticles and their application

Milojević, Marko; Gradišnik, Lidija; Stergar, Janja; Klemen, Maša Skelin; Stožer, Andraž; Vesenjak, Matej; Dubrovski, Polona Dobnik; Maver, Tina; Mohan, Tamilselvan; Stana‐Kleinschek, Karin; Maver, Uroš

doi:10.1016/j.apsusc.2019.05.283

Cited by 37 publications

(36 citation statements)

References 52 publications

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“…Nevertheless, many studies have shown that for wound healing purposes, polysaccharides ALG and CMC also have a similarly positive effect, which is related to their similarity in many aspects to the natural skin ECM. These similarities include a good mechanical strength and stiffness, biocompatibility, and providing a highly hydrated environment, favorable for the skin [23][24][25][26][27]. Still, the application of hydrogel-based bioinks in fabrication of clinically relevant 3D skin constructs can be challenging, which is mostly related to their suboptimal printability for this purpose.…”

Section: Introductionmentioning

confidence: 99%

“…To compensate the inability to maintain a uniform 3D structure, hydrogels are often printed in combination with other materials. Recently, it has been demonstrated that shortcomings associated with using hydrogels, especially alginate-derived, can be overcome by adding cellulose nanofibrils [24,28,30]. Depending on the cellulose source and its processing conditions, these nanofibrills can be divided into three main categories: microfibrillated cellulose, nanocrystalline cellulose, and bacterial nanocellulose.…”

Section: Introductionmentioning

confidence: 99%

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Polysaccharide-Based Bioink Formulation for 3D Bioprinting of an In Vitro Model of the Human Dermis

et al. 2020

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Limitations in wound management have prompted scientists to introduce bioprinting techniques for creating constructs that can address clinical problems. The bioprinting approach is renowned for its ability to spatially control the three-dimensional (3D) placement of cells, molecules, and biomaterials. These features provide new possibilities to enhance homology to native skin and improve functional outcomes. However, for the clinical value, the development of hydrogel bioink with refined printability and bioactive properties is needed. In this study, we combined the outstanding viscoelastic behavior of nanofibrillated cellulose (NFC) with the fast cross-linking ability of alginate (ALG), carboxymethyl cellulose (CMC), and encapsulated human-derived skin fibroblasts (hSF) to create a bioink for the 3D bioprinting of a dermis layer. The shear thinning behavior of hSF-laden bioink enables construction of 3D scaffolds with high cell density and homogeneous cell distribution. The obtained results demonstrated that hSF-laden bioink supports cellular activity of hSF (up to 29 days) while offering proper printability in a biologically relevant 3D environment, making it a promising tool for skin tissue engineering and drug testing applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polysaccharide-Based Bioink Formulation for 3D Bioprinting of an In Vitro Model of the Human Dermis

et al. 2020

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“…As can be concluded from the currently available literature, which grew for the last couple of years, NiCu MNPs could be among future formulations for use as MH formulations, drug delivery systems, or other applications. With an already proven track record of successful application (currently only in in vitro and animal models), the potential of NiCu MNPs will only grow; many research groups are now testing their suitability using various additional tissue models to determine their application as part of the abovementioned uses or even in regenerative medicine, which was not addressed above [114].…”

Section: Discussionmentioning

confidence: 99%

The Potential Biomedical Application of NiCu Magnetic Nanoparticles

2019

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Magnetic nanoparticles became increasingly interesting in recent years as a result of their tailorable size-dependent properties, which enable their use in a wide range of applications. One of their emerging applications is biomedicine; in particular, bimetallic nickel/copper magnetic nanoparticles (NiCu MNPs) are gaining momentum as a consequence of their unique properties that are suitable for biomedicine. These characteristics include stability in various chemical environments, proven biocompatibility with various cell types, and tunable magnetic properties that can be adjusted by changing synthesis parameters. Despite the obvious potential of NiCu MNPs for biomedical applications, the general interest in their use for this purpose is rather low. Nevertheless, the steadily increasing annual number of related papers shows that increasingly more researchers in the biomedical field are studying this interesting formulation. As with other MNPs, NiCu-based formulations were examined for their application in magnetic hyperthermia (MH) as one of their main potential uses in clinics. MH is a treatment method in which cancer tissue is selectively heated through the localization of MNPs at the target site in an alternating magnetic field (AMF). This heating destroys cancer cells only since they are less equipped to withstand temperatures above 43 °C, whereas this temperature is not critical for healthy tissue. Superparamagnetic particles (e.g., NiCu MNPs) generate heat by relaxation losses under an AMF. In addition to MH in cancer treatment, which might be their most beneficial potential use in biomedicine, the properties of NiCu MNPs can be leveraged for several other applications, such as controlled drug delivery and prolonged localization at a desired target site in the body. After a short introduction that covers the general properties of NiCu MNPs, this review explores different synthesis methods, along with their main advantages and disadvantages, potential surface modification approaches, and their potential in biomedical applications, such as MH, multimodal cancer therapy, MH implants, antibacterial activity, and dentistry.

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“…For extrusion, hydrogels need to be prepared such that the viscoelastic properties and shear modulus during extrusion is appropriate for proper printing of features at high fidelity and resolution. Approaches include tuning the degree of crosslinking of the bioink and formation of composites or mixtures such as with NiCu NPs [237], e-polylysine [238], carrageenan [239], gelatin [240][241][242], and nanocellulose [243,244]. Rheological studies show composition ratio, printing temperature, extrusion pressure, and crosslinking concentration affect fidelity and resolution [239,240,242,245].…”

Section: Process and Materialsmentioning

confidence: 99%

“…Inclusion of NiCu [237], e-polylysine [238], carrageenan [239], gelatin [240][241][242], cellulose [243,244], PVA [252], TiO 2 [253], β-TCP [253]; Feature size 150 µm, E: 280 kPa [240] Myoblasts [242]; endothelial cells [259]; E.coli [260], growth factor [250], human adipose stem cells [248]; human induced pluripotent stem cells [261]; chondrocytes [243,244,251]; Schwann cells [265] Cellulose (4.3.4) Mixed with alginate [244,[266][267][268][269][270][271][272]; As reinforcement material, E: 2.5-22.5 kPa [281] Feature size 500 µm [280]; Tensile E: 0.67-0.63 GPa [282] Chondrocytes for cartilage tissue engineering [244,269,271]; human induced pluripotent stem cells, bone-marrow hMSCs [272]; pancreatic cancer cells [268]; fibroblast and hepatoma cells [270]; NIH 3T3 cells [274] Collagen (4.4.1)…”

Section: ) Applicationsmentioning

confidence: 99%

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

2019

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The realization of biomimetic microenvironments for cell biology applications such as organ-on-chip, in vitro drug screening, and tissue engineering is one of the most fascinating research areas in the field of bioengineering. The continuous evolution of additive manufacturing techniques provides the tools to engineer these architectures at different scales. Moreover, it is now possible to tailor their biomechanical and topological properties while taking inspiration from the characteristics of the extracellular matrix, the three-dimensional scaffold in which cells proliferate, migrate, and differentiate. In such context, there is therefore a continuous quest for synthetic and nature-derived composite materials that must hold biocompatible, biodegradable, bioactive features and also be compatible with the envisioned fabrication strategy. The structure of the current review is intended to provide to both micro-engineers and cell biologists a comparative overview of the characteristics, advantages, and drawbacks of the major 3D printing techniques, the most promising biomaterials candidates, and the trade-offs that must be considered in order to replicate the properties of natural microenvironments.

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Development of multifunctional 3D printed bioscaffolds from polysaccharides and NiCu nanoparticles and their application

Cited by 37 publications

References 52 publications

Polysaccharide-Based Bioink Formulation for 3D Bioprinting of an In Vitro Model of the Human Dermis

Polysaccharide-Based Bioink Formulation for 3D Bioprinting of an In Vitro Model of the Human Dermis

The Potential Biomedical Application of NiCu Magnetic Nanoparticles

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

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