Development of monoclonal antibodies for the rapid identification of epizootic <i>Vibrio</i> species

Hanna, Peter; Altmann, Klaus; Chen, D.; Smith, Arthur A.; Cosic, S.; Moon, Park Jong; Hammond, Laurie

doi:10.1111/j.1365-2761.1992.tb00637.x

Cited by 14 publications

(7 citation statements)

References 20 publications

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“…Serological assays indicated that the V. ordalii isolates were highly homogeneous because whole cells and 0 antigens produced strong slide agglutination and ELISA with antisera raised against isolate 1 and CECT-582. These two assays showed that the ten V. ordalii strains were antigenically homogeneous and no serotypes were detected, as described by other researchers (Toranzo et al, 1987;Hanna et al, 1991;Chen et al, 1992;Grisez and Ollevier, 1995;Austin et al, 1997).…”

Section: Discussionsupporting

confidence: 80%

See 1 more Smart Citation

Identification and Genotyping of Vibrio ordalii: A Comparison of Different Methods

Akayli¹,

Timur²,

Albayrak³

et al. 2010

Israeli Journal of Aquaculture - Bamidgeh

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As from January 2010 The Israeli Journal of Aquaculture-Bamidgeh (IJA) will be published exclusively as an on-line Open Access (OA) quarterly accessible by all AquacultureHub (http://www.aquaculturehub.org) members and registered individuals and institutions. Please visit our website (http://siamb.org.il) for free registration form, further information and instructions. This transformation from a subscription printed version to an on-line OA journal, aims at supporting the concept that scientific peer-reviewed publications should be made available to all, including those with limited resources. The OA IJA does not enforce author or subscription fees and will endeavor to obtain alternative sources of income to support this policy for as long as possible.

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Section: Discussionsupporting

confidence: 80%

“…Enzyme-linked immunosorbent assay (ELISA). An indirect ELISA was used to detect the antibody in the antirabbit serum samples against Vibrio species, as described by Arkoosh and Kaattari (1990) and Hanna et al (1991). Each bacterial suspension was added to each well of a 96-well microtiter plate.…”

Section: Methodsmentioning

confidence: 99%

Identification and Genotyping of Vibrio ordalii: A Comparison of Different Methods

Akayli¹,

Timur²,

Albayrak³

et al. 2010

Israeli Journal of Aquaculture - Bamidgeh

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“…Vibriosis is caused by species of Vibrio , namely by Vibrio anguillarum [ 7 – 9 ]. Others species of Vibrio , such as V. alginolitycus , V. anguillarum , V. carchariae , V. salmonicida , V. damsela , V. ordalii , V. parahemolyticus and V. vulnificus , causes also important infections in several species of fish [ 10 , 11 ]. Photobacteriosis is caused by Photobacterium damselae subsp.…”

Section: Introductionmentioning

confidence: 99%

Bacteriophages with Potential for Inactivation of Fish Pathogenic Bacteria: Survival, Host Specificity and Effect on Bacterial Community Structure

et al. 2011

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Phage therapy may represent a viable alternative to antibiotics to inactivate fish pathogenic bacteria. Its use, however, requires the awareness of novel kinetics phenomena not applied to conventional drug treatments. The main objective of this work was to isolate bacteriophages with potential to inactivate fish pathogenic bacteria, without major effects on the structure of natural bacterial communities of aquaculture waters. The survival was determined in marine water, through quantification by the soft agar overlay technique. The host specificity was evaluated by cross infection. The ecological impact of phage addition on the structure of the bacterial community was evaluated by DGGE of PCR amplified 16S rRNA gene fragments. The survival period varied between 12 and 91 days, with a higher viability for Aeromonas salmonicida phages. The phages of Vibrio parahaemolyticus and of A. salmonicida infected bacteria of different families with a high efficacy of plating. The specific phages of pathogenic bacteria had no detectable impact on the structure of the bacterial community. In conclusion, V. parahaemolyticus and A. salmonicida phages show good survival time in marine water, have only a moderated impact on the overall bacterial community structure and the desired specificity for host pathogenic bacteria, being potential candidates for therapy of fish infectious diseases in marine aquaculture systems.

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“…V. parahaemolyticus, indeed, is a human bacterial pathogen widely occurring in marine environments, frequently isolated from a variety of seafood, including bivalves, crustaceans, and fish [67]. Vibriosis is currently responsible for most disease outbreaks in aquaculture [31][32][33][34]68].…”

Section: Discussionmentioning

confidence: 99%

“…In highly anthropized marine environments, such as intensive or confined mariculture systems, the excessive release of excreta from farmed species and organic matter from uneaten feed create favorable conditions for pathogenic bacteria growth, especially Vibrio, responsible for diseases and high mortality in target species, with consequent economic losses [31][32][33][34][35][36][37]. To overcome this problem, the use of antibiotics has spread despite the increase in production costs and the negative consequences on farmed species and the surrounding environment.…”

Section: Introductionmentioning

confidence: 99%

Filtering Activity and Nutrient Release by the Keratose Sponge Sarcotragus spinosulus Schmidt, 1862 (Porifera, Demospongiae) at the Laboratory Scale

Trani

Corriero

Pinto

et al. 2021

JMSE

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Sponges are an important constituent of filter-feeder benthic communities, characterized by high ecological plasticity and abundance. Free bacteria constitute an important quota of their diet, making them excellent candidates in aquaculture microbial bioremediation, where bacteria can be a serious problem. Although there are studies on this topic, certain promising species are still under investigation. Here we report applied microbiological research on the filtering activity of Sarcotragus spinosulus on two different concentrations of the pathogenic bacterium Vibrio parahaemolyticus in a laboratory experiment. To evaluate the effects of the filtration on the surrounding nutrient load, the release of ammonium, nitrate, and phosphate was also measured. The results obtained showed the efficient filtration capability of S. spinosulus as able to reduce the Vibrio load with a maximum retention efficiency of 99.72% and 99.35% at higher and lower Vibrio concentrations, respectively, and remarkable values of clearance rates (average maximum value 45.0 ± 4.1 mL h−1 g DW−1) at the highest Vibrio concentration tested. The nutrient release measured showed low values for each considered nutrient category at less than 1 mg L−1 for ammonium and phosphate and less than 5 mg L−1 for nitrate. The filtering activity and nutrient release by S. spinosulus suggest that this species represents a promising candidate in microbial bioremediation, showing an efficient capability in removing V. parahaemolyticus from seawater with a contribution to the nutrient load.

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Development of monoclonal antibodies for the rapid identification of epizootic Vibrio species

Cited by 14 publications

References 20 publications

Identification and Genotyping of Vibrio ordalii: A Comparison of Different Methods

Identification and Genotyping of Vibrio ordalii: A Comparison of Different Methods

Bacteriophages with Potential for Inactivation of Fish Pathogenic Bacteria: Survival, Host Specificity and Effect on Bacterial Community Structure

Filtering Activity and Nutrient Release by the Keratose Sponge Sarcotragus spinosulus Schmidt, 1862 (Porifera, Demospongiae) at the Laboratory Scale

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