Identification and Genotyping of Vibrio ordalii: A Comparison of Different Methods

Akayli, Tülay; Timur, Gülşen; Albayrak, Gülruh; Aydemir, Birsen Çakir

doi:10.46989/001c.20577

Cited by 5 publications

(2 citation statements)

References 20 publications

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“…Vibrio ordalii, formerly Vibrio anguillarum biovar II (Schiewe et al 1981), is an important causative agent of vibriosis in several salmonid species cultured in the USA, Japan, Australia, New Zealand and Chile (Harrell et al 1976, Ransom et al 1984, Toranzo et al 1997, Colquhoun et al 2004, although it has also been reported as a pathogen for other fish species such as gilthead sea bream Sparus aurata in Turkey (Akayli et al 2010). This pathogen is a Gram-negative, motile, rod-shaped bacterium that is fermentative, catalase and oxidase positive, and sensitive to the vibriostatic agent O/129 (Farmer et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Iron assimilation and siderophore production by Vibrio ordalii strains isolated from diseased Atlantic salmon Salmo salar in Chile

Ruiz

Balado²,

Toranzo³

et al. 2016

Dis. Aquat. Org.

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Vibrio ordalii is the causative agent of vibriosis in several cultured salmonid species worldwide. Despite its impact on aquaculture, relatively little information is available about its virulence factors. The present study demonstrates for the first time that V. ordalii possesses different systems of iron acquisition, one involving siderophore synthesis and another one that uses direct binding of heme to use iron. Using 6 strains of V. ordalii from Atlantic salmon Salmo salar and the V. ordalii type strain, we could demonstrate that all strains could grow in presence of the chelating agent 2,2'-dipyridyl and produced siderophores in solid and liquid media. Cross-feeding assays among V. ordalii strains evidenced variability in the siderophores produced. Bioassays and PCR data suggest that V. ordalii could produce a siderophore with a structure similar to piscibactin, although the production of a second siderophore in certain strains cannot be discarded. Furthermore, all strains were able to use hemin and hemoglobin as the only iron sources, although the cell yield was higher when using hemoglobin. A hemin-binding assay indicated the presence of constitutive heme-binding molecules at the cell surface of V. ordalii. Virulence tests using rainbow trout as a model of infection revealed a clear relationship between iron-uptake ability and pathogenicity in V. ordalii.

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Section: Introductionmentioning

confidence: 99%

Iron assimilation and siderophore production by Vibrio ordalii strains isolated from diseased Atlantic salmon Salmo salar in Chile

Ruiz

Balado²,

Toranzo³

et al. 2016

Dis. Aquat. Org.

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“…Paillard et al recognized that the emergence of vibrios as etiological agents of diseases is likely to increase over the coming years due to ocean warming [9]. Classical vibriosis is generally characterized by lethargic movement of affected fish [10][11][12], the presence of various skin ulcerations [12][13][14][15], rotting fins [14,16,17], and pigmentation of the body [10,13,14,18]. Internal organs appear enlarged, hemorrhagic and congested, particularly the liver, kidney, and spleen [11,12,18].…”

Section: Introductionmentioning

confidence: 99%

The Isolation of Vibrio crassostreae and V. cyclitrophicus in Lesser-Spotted Dogfish (Scyliorhinus canicula) Juveniles Reared in a Public Aquarium

Tomasoni

Esposito

Mugetti

et al. 2022

JMSE

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The genus Vibrio currently contains 147 recognized species widely distributed, including pathogens for aquatic organisms. Vibrio infections in elasmobranchs are poorly reported, often with identifications as Vibrio sp. and without detailed diagnostic insights. The purpose of this paper is the description of the isolation and identification process of Vibrio spp. following a mortality event of Scyliorhinus canicula juvenile reared in an Italian public aquarium. Following investigations aimed at excluding the presence of different pathogens of marine fish species (parasites, bacteria, Betanodavirus), several colonies were isolated and subjected to species identification using the available diagnostic techniques (a biochemical test, MALDI-TOF MS, and biomolecular analysis). Discrepancies were observed among the methods; the limits of biochemistry as a unique tool for Vibrio species determination were detected through statistical analysis. The use of the rpoB gene, as a diagnostic tool, allowed the identification of the isolates as V. crassostreae and V. cyclotrophicus. Although the pathogenic role of these microorganisms in lesser-spotted dogfish juveniles has not been demonstrated, and the presence of further pathogens cannot be excluded, this study allowed the isolation of two Vibrio species in less-studied aquatic organisms, highlighting the weaknesses and strengths of the different diagnostic methods applied.

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Effects of a short pulse administration ofUlva rigidaon innate immune response and intestinal microbiota inSparus auratajuveniles

Abdala-Díaz

García‐Márquez

Rico

et al. 2021

Aquaculture Research

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Given the potential of algae as a new aquafeed ingredient, this study evaluated the effect of the tank-cultivated macroalgae Ulva rigida C. Agardh (Chlorophyta) on innate immune response, intestinal microbiota and their resistance against Photobacterium damselae subsp. piscicida in juvenile gilthead seabream (Sparus aurata L.) after a short pulse of administration. An algae-free diet was used as control, and an experimental diet was formulated containing 25% of U. rigida (UL-25). After 2, 7, 15 and 30 days biological samples were taken. No statistical significance (p > 0.05) was detected in respiratory burst activity. Diet UL-25 significantly enhanced alternative complement pathway activity respect to control diet from the beginning of the experiment (p < 0.05). When fish were challenged against P. damselae subsp. piscicida, UL-25-fed fish were more resistant against the pathogen than the fish fed control diet, obtaining a relative survival percentage of 54.55%. The ability of UL-25 diet to modify the intestinal microbiota was also confirmed. In conclusion, the use of a short pulse of dietary administration of 25% U. rigida in S. aurata diet modulates the immune response, intestinal microbiota and reduced the mortality rate during P. damselae subsp. piscicida infection.

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Identification and Genotyping of Vibrio ordalii: A Comparison of Different Methods

Cited by 5 publications

References 20 publications

Iron assimilation and siderophore production by Vibrio ordalii strains isolated from diseased Atlantic salmon Salmo salar in Chile

Iron assimilation and siderophore production by Vibrio ordalii strains isolated from diseased Atlantic salmon Salmo salar in Chile

The Isolation of Vibrio crassostreae and V. cyclitrophicus in Lesser-Spotted Dogfish (Scyliorhinus canicula) Juveniles Reared in a Public Aquarium

Effects of a short pulse administration ofUlva rigidaon innate immune response and intestinal microbiota inSparus auratajuveniles

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