Development of methodology for resistance screening of chickpea genotypes collected in Turkey to the root lesion nematode, Pratylenchus thornei Sher & Allen, 1953 (Tylenchida: Pratylenchidae)

Abstract

The root lesion nematode, Pratylenchus thornei Sher & Allen, 1953 (Tylenchida: Pratylenchidae) are considered economically important plant parasitic nematodes affecting chickpea [Cicer arietinum L. (Fabales: Fabaceae)] production. A major strategy to develop resistance to root lesion nematodes in chickpea is to assess and exploit their natural variation. Therefore, nine accessions of wild Cicer reticulatum Ladiz. (Fabales: Fabaceae), Cicer echinospermum P.H.Davis (Fabales: Fabaceae) and domesticate C. arietinum were assessed for resistance to P. thornei according to multiplication rate of the nematode. This study was conducted during 2014-2015 to detect the suitable initial inoculum density (150, 225 and 300) per plant duration of experiment (16 and 20 weeks) for resistance test to P. thornei in chickpea cultivars. There was no significant difference between growing times 16 and 20 weeks and between the initial inoculum density of 225 and 300 nematodes. The only significant difference was observed at a low initial inoculum density of 150 nematodes in all tested cultivars. Therefore, the initial inoculum density of 225 and the growing time of 16 weeks were selected to access of chickpea genotypes for resistance study to P. thornei.

Investigation of the development of root lesion nematodes, Pratylenchus spp. (Tylenchida: Pratylenchidae) in three chickpea cultivars

“…According to the results, there was a significant difference between nematode species in the root sample, and population density of nematodes differed between cultivars of C. echinospermum and C. reticulatum studied. Behmand et al (2020) indicated that the population density of root lesion nematodes was differed with species (P. neglectus and P. thornei) and Cicer species. The population density of P. thornei in C. echinospermum was higher than the population density in C. reticulatum.…”

“…One week after sowing, the seedlings were inoculated with number of 225 nematodes consisting of mixed development stages in 1 ml of water (Behmand et al, 2020).…”

“…Chickpea cultivars that were determined previously as resistant and susceptible to P. neglectus, P. penetrans and P. thornei by Behmand (2020) were screened against these root lesion nematodes (Table 1). These cultivars used in an experiment at Plant Protection Department, Çukurova University in 2018 -2019.…”