Development of Methodology Based on Commercialized SERS-Active Substrates for Rapid Discrimination of Poxviridae Virions

Alexander, Troy A.

doi:10.1021/ac702464w

Cited by 48 publications

(37 citation statements)

References 40 publications

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“…The chemical enhancement theory attributes the SERS enhancement effects to charge-transfer between adsorbed analyte molecules and roughened metal surface. Although SERS enhancement effect is a rather complicated phenomenon with many questions unresolved, SERS has a great promise as an analytical method in detecting and determining trace amounts of toxicant or prohibited drugs as indicated in some research reports over a diverse fields [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Application of surface enhanced Raman spectroscopy for analyses of restricted sulfa drugs

Lai

Zhai

Zhang

et al. 2011

Sens. & Instrumen. Food Qual.

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The presence of sulfonamide residues in muscle foods is an important concern for consumers and regulatory agencies since these residues may pose potential health risks and result in an increase of drug-resistant bacteria. Surface enhanced Raman spectroscopy (SERS) was applied to analyze three sulfa drugs including sulfamerazine, sulfamethazine and sulfamethoxazole with concentrations ranging from 10 ng mL -1 to 5 lg mL -1 . Partial least squares regression (PLS) and principal component analysis (PCA) were used for the spectral data analyses. The three sulfa drugs could be detected at concentration levels as low as 10 ng mL -1 . For the quantitative analyses, the R 2 values of actual sulfa drug concentrations versus their concentrations predicted by the PLS models ranged from 0.8149 to 0.9009. Plotting of principal components based upon PCA showed clear, separated clusters between different sulfonamides. This study indicated potential for detection and determination of trace amounts of prohibited or restricted drugs with SERS technology.

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Section: Introductionmentioning

confidence: 99%

Application of surface enhanced Raman spectroscopy for analyses of restricted sulfa drugs

Lai

Zhai

Zhang

et al. 2011

Sens. & Instrumen. Food Qual.

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“…Raman spectroscopy has also been employed to investigate nonviral protein structural variations (20). Recently, surface-enhanced Raman scattering (SERS) spectroscopy and tip-enhanced Raman scattering (TERS) spectroscopy have been employed to identify and discriminate different types of viruses, such as Poxviridae virions (21), measles viruses (22), rotavirus (23), respiratory syncytial viruses (24,25), tobacco mosaic virus (26), and avipoxvirus (27). Moreover, for nonenveloped viruses, Raman spectroscopy has made identification of single viral particles possible (26).…”

mentioning

confidence: 99%

Detection of Receptor-Induced Glycoprotein Conformational Changes on Enveloped Virions by Using Confocal Micro-Raman Spectroscopy

Liu

Benavides-Montaño

et al. 2013

J Virol

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Conformational changes in the glycoproteins of enveloped viruses are essential for membrane fusion, a critical step during both viral entry and the pathognomonic cell-cell fusion (syncytia) associated with many viral infections, such as those of the paramyxoviruses. However, technologies that detect glycoprotein conformational changes on actual enveloped virions are difficult and time-consuming for those viruses for which such detection is possible (1-6). Additionally, there is a great need for rapid identification and characterization of virions in medical, veterinary, or food samples. Having a method with these capabilities would advance the fields of virus diagnosis and analysis.Our model, Nipah virus (NiV), is an enveloped virus in the important Paramyxoviridae family, which comprises human and veterinary enveloped viruses such as measles virus, mumps virus, Newcastle disease virus, respiratory syncytial virus, canine distemper virus, the metapneumoviruses, the human parainfluenza viruses, Hendra virus (HeV), and NiV (6-8). NiV is an emerging zoonotic virus in the Henipavirus genus that causes severe illness in humans, characterized by encephalitis and respiratory disease associated with syncytium formation (7, 9). Although NiV causes 40 to 75% mortality in humans, there is no approved treatment; thus, NiV is classified as a biosafety level 4 agent and a priority pathogen in the NIH/NIAID agenda. Additionally, because paramyxoviruses are relatively stable in aerosols and NiV is capable of animal-to-animal, animal-to-human, and human-to-human transmission, NiV is considered a potential agro-and/or bioterrorism agent (7).Conformational changes of the viral glycoproteins are required for viral entry and cell-cell fusion. However, it is difficult to obtain X-ray crystal structural information from intact full-length glycoproteins because their hydrophobic transmembrane regions are embedded in a lipid membrane. Therefore, structural studies have been skewed toward ectodomain glycoprotein forms because it is relatively more straightforward to obtain structural information for them. Viral glycoprotein conformational changes have typically been observed either by analysis of viral glycoprotein soluble ectodomain forms (e.g., see references 10-12) or by analysis of full-length wild-type glycoproteins expressed on cell surfaces (e.g., see reference 13). Although soluble ectodomains normally bind their respective cell receptors, it is often not possible to assess how accurately their structures and structural changes compare with those of their membrane-bound full-length wild-type counterparts. Moreover, the tendency of soluble glycoprotein ectodomains to adopt postfusion conformations in many cases limits our ability to detect and characterize essential glycoprotein receptorinduced conformational changes (12,14). Analysis of receptorinduced conformational changes of full-length wild-type glycoproteins embedded in cellular or viral membranes is preferred, and even for such analyses, there may be differences in the role...

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“…Innovative detection mechanisms, such as using cicada nanopillar arrays as a substrate scaffold [246], are being developed. Commercially available substrates detect bovine papular stomatitis, pseudocowpox, and Yaba monkey tumor viruseswithout the need for reagents or labels and can be used to identify an unknown parapoxvirus [255]. While virus detection through SERS is moving to development using antigens [256] or nucleic acid, specific and sensitive whole virus detection is possible.…”

Section: Detection Of Other Biologically Relevant Nanoparticlesmentioning

confidence: 99%

Fundamentals and applications of SERS-based bioanalytical sensing

et al. 2017

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Abstract:Plasmonics is an emerging field that examines the interaction between light and metallic nanostructures at the metal-dielectric interface. Surface-enhanced Raman scattering (SERS) is a powerful analytical technique that uses plasmonics to obtain detailed chemical information of molecules or molecular assemblies adsorbed or attached to nanostructured metallic surfaces. For bioanalytical applications, these surfaces are engineered to optimize for high enhancement factors and molecular specificity. In this review we focus on the fabrication of SERS substrates and their use for bioanalytical applications. We review the fundamental mechanisms of SERS and parameters governing SERS enhancement. We also discuss developments in the field of novel SERS substrates. This includes the use of different materials, sizes, shapes, and architectures to achieve high sensitivity and specificity as well as tunability or flexibility. Different fundamental approaches are discussed, such as label-free and functional assays. In addition, we highlight recent relevant advances for bioanalytical SERS applied to small molecules, proteins, DNA, and biologically relevant nanoparticles. Subsequently, we discuss the importance of data analysis and signal detection schemes to achieve smaller instruments with low cost for SERS-based point-of-care technology developments. Finally, we review the main advantages and challenges of SERS-based biosensing and provide a brief outlook.

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Development of Methodology Based on Commercialized SERS-Active Substrates for Rapid Discrimination of Poxviridae Virions

Cited by 48 publications

References 40 publications

Application of surface enhanced Raman spectroscopy for analyses of restricted sulfa drugs

Application of surface enhanced Raman spectroscopy for analyses of restricted sulfa drugs

Detection of Receptor-Induced Glycoprotein Conformational Changes on Enveloped Virions by Using Confocal Micro-Raman Spectroscopy

Fundamentals and applications of SERS-based bioanalytical sensing

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