Development of Mesoporous Silica Nanoparticles of Tunable Pore Diameter for Superior Gemcitabine Drug Delivery in Pancreatic Cancer Cells

Saini, Kusum; Prabhuraj, R S; Bandyopadhyaya, Rajdip

doi:10.1166/jnn.2020.17381

Cited by 36 publications

(20 citation statements)

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“…The samples were designated as NH 2 -MSN (without decane) and NH 2 -MSN-dec (with decane). A detailed optimization process of internal pore diameter, obtained by varying the alkane/CTAB molar ratio, has been published by us recently …”

Section: Methodsmentioning

confidence: 99%

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Transferrin-Conjugated Polymer-Coated Mesoporous Silica Nanoparticles Loaded with Gemcitabine for Killing Pancreatic Cancer Cells

Saini

Bandyopadhyaya

2019

ACS Appl. Nano Mater.

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In order to achieve superior delivery of the anticancer drug gemcitabine to Mia PaCa-2 pancreatic cancer cell lines, we have synthesized transferrin (Tf)-conjugated, polymer-coated, mesoporous silica nanoparticles (MSNs), with optimization of multiple parameters. The following challenges were thereby addressed to improve the efficacy of gemcitabine delivery: (i) optimization of internal pore diameter (within the range 2.5–5.2 nm) and amine functionalization of MSN (NH2-MSN) to improve drug loading and its controlled release, (ii) coating of MSN with pH-sensitive polymers such as either chitosan or poly(d,l-lactide-co-glycolide) (PLGA), so as to prevent premature release of the drug at physiological pH 7.4 and to also achieve its controlled release at a lower pH 5.5 (extracellular cancer cell pH), and finally, (iii) conjugation of Tf ligand on this optimized, polymer-coated MSN, for better uptake of MSN by the MIA PaCa-2 cells, through ligand–receptor interactions. Consequently, the highest drug loading of 27.2% could be achieved for the sample having amine-functionalized MSN with larger pore diameter of 5.2 nm, compared to only 13.1% for small pore diameter of 2.5 nm, demonstrating better drug loading with larger pores. Furthermore, with coating of PLGA on MSN, a more controlled and desirable constant release rate of gemcitabine with time was achieved at pH 5.5, compared to a miniscule 3% of undesirable drug release at physiological pH 7.4. Finally, for Tf-conjugated, polymer-coated MSN, 70–75% of MIA PaCa-2 cells killing was achieved, as compared to 60% without Tf conjugation. This was due to improved uptake of nanoparticles by cancer cells, via ligand–receptor interactions. Thus, the above-optimized MSN-based gemcitabine delivery system can be a superior formulation to contain pancreatic cancer cell growth.

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Section: Methodsmentioning

confidence: 99%

“…A detailed optimization process of internal pore diameter, obtained by varying the alkane/CTAB molar ratio, has been published by us recently. 46 Drug Accommodation in NH 2 -MSN. NH 2 -MSN and NH 2 -MSNdec samples were loaded with gemcitabine using the method of immersion.…”

Section: Methodsmentioning

confidence: 99%

Transferrin-Conjugated Polymer-Coated Mesoporous Silica Nanoparticles Loaded with Gemcitabine for Killing Pancreatic Cancer Cells

Saini

Bandyopadhyaya

2019

ACS Appl. Nano Mater.

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“…These side effects are because some drugs that have low bioavailability, so they must be administered in high doses. For this reason, in the last decades, the challenge has been focused on the investigation of the targeted drug delivery systems [5][6][7][8][9][10][11][12][13]. There is a large variety of organic systems that have been considered as drug delivery systems, such as micelles, liposomes, and polymeric nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Dendrimers: Amazing Platforms for Bioactive Molecule Delivery Systems

Sandoval-Yáñez

Rodríguez

2020

Materials

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Today, dendrimers are the main nanoparticle applied to drug delivery systems. The physicochemical characteristics of dendrimers and their versatility structural modification make them attractive to applied as a platform to bioactive molecules transport. Nanoformulations based on dendrimers enhance low solubility drugs, arrival to the target tissue, drugs bioavailability, and controlled release. This review describes the latter approaches on the transport of bioactive molecules based on dendrimers. The review focus is on the last therapeutic strategies addressed by dendrimers conjugated with bioactive molecules. A brief review of the latest studies in therapies against cancer and cardiovascular diseases, as well as future projections in the area, are addressed.

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“…These advantages have attracted attention in the field of application of drug delivery systems (DDS) [6,7]. Among various mesoporous materials [8][9][10], the unique mesopore structures [11] and facile surface functional properties [12,13] of mesoporous silica nanoparticles (MSN) make it feasible for use in the design of diverse stimuli-responsive gatekeepers. These structures control the opening and closing of the mesopores for a triggered release of drugs loaded inside the porous particles [14,15].…”

Section: Introductionmentioning

confidence: 99%

pH and Redox Dual-Responsive MSN-S-S-CS as a Drug Delivery System in Cancer Therapy

Xiao

Chang

et al. 2020

Materials

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In order to achieve a controlled release drug delivery system (DDS) for cancer therapy, a pH and redox dual-responsive mesoporous silica nanoparticles (MSN)-sulfur (S)-S- chitosan (CS) DDS was prepared via an amide reaction of dithiodipropionic acid with amino groups on the surface of MSN and amino groups on the surface of CS. Using salicylic acid (SA) as a model drug, SA@MSN-S-S-CS was prepared by an impregnation method. Subsequently, the stability, swelling properties and drug release properties of the DDS were studied by x-ray diffraction, scanning electron microscopy, Fourier transform infrared microspectroscopy, size and zeta potential as well as Brunauer–Emmett–Teller surface area. Pore size and volume of the composites decreased after drug loading but maintained a stable structure. The calculated drug loading rate and encapsulation efficiency were 8.17% and 55.64%, respectively. The in vitro drug release rate was 21.54% in response to glutathione, and the release rate showed a marked increase as the pH decreased. Overall, double response functions of MSN-S-S-CS had unique advantages in controlled drug delivery, and may be a new clinical application of DDS in cancer therapy.

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Development of Mesoporous Silica Nanoparticles of Tunable Pore Diameter for Superior Gemcitabine Drug Delivery in Pancreatic Cancer Cells

Cited by 36 publications

References 0 publications

Transferrin-Conjugated Polymer-Coated Mesoporous Silica Nanoparticles Loaded with Gemcitabine for Killing Pancreatic Cancer Cells

Transferrin-Conjugated Polymer-Coated Mesoporous Silica Nanoparticles Loaded with Gemcitabine for Killing Pancreatic Cancer Cells

Dendrimers: Amazing Platforms for Bioactive Molecule Delivery Systems

pH and Redox Dual-Responsive MSN-S-S-CS as a Drug Delivery System in Cancer Therapy

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