Development of membranes based on carboxymethyl cellulose/acetylated arrowroot starch containing bromelain extract carried on nanoparticles and liposomes

Miranda, Ileana; Santana, Fernando Rocha; Camilloto, Geany Peruch; Detoni, Chaline; Souza, Fernanda Vidigal Duarte; Albuquerque, Elaine Christine de Magalhães Cabral; Alves, Sara Lima; Neco, Glaucia Lais; Lima, Flávia Oliveira de; Assis, Sandra Aparecida de

doi:10.1016/j.xphs.2021.02.031

Cited by 7 publications

(5 citation statements)

References 34 publications

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“…This process can be accelerated by using an ultrasound bath. The resulting large MLVs are subjected to sonication, finally obtaining small unilamellar vesicles (SUVs) [ 94 , 97 ].…”

Section: Preparation Methods Of Vesicular Nanosystemsmentioning

confidence: 99%

“…Thus, the thin film can be hydrated with the PBS HEs or PCs solution, such as Carpobrotus edulis [ 90 ] powder extract, anthocyanin complex [ 85 ], madecassoside [ 44 ], essential oil derivative alpha-bisabolol [ 99 ], with the ethanolic solution of the PC [ 74 ], with the aqueous solution of HEs ( Narcissus tazetta extract [ 84 ]), or with the HE itself (Danggui Buxue extract [ 92 ], Salvia aramiensis extract [ 58 ], or Aloe vera leaf gel extract [ 91 ]). In some other cases, the extract can be added at the end and stirred with the vesicles dispersion ( Moringa oleifera extract [ 29 ] and bromelain extract [ 94 ]).…”

Section: Preparation Methods Of Vesicular Nanosystemsmentioning

confidence: 99%

“…For chronic wounds and those with impaired healing, such as bedsores, venous ulcers, or diabetic foot ulcers, it has been found that wound dressings’ application has significantly reduced the cost of the treatment. Modern dressings can incorporate and deliver active substances, such as antimicrobial, anti-inflammatory, or analgesic agents, at the wound site [ 94 , 113 , 114 ]. Including VNs in modern dressings can be an interesting field of research, because this may provide targeted and sustained delivery of constant concentrations of the drug on the wound site together with the improved penetration of the drug, swelling in the presence of wound exudates, better protection of active ingredients, and thereby favor the wound healing [ 112 ].…”

Section: Topical Delivery Systems Containing Herbal Extractsmentioning

confidence: 99%

“…In addition, other studies describing the incorporation of lipid vesicles in modern dressings for wound healing have shown promising results [ 117 , 118 , 119 , 120 ]. Although, a thorough search of the relevant literature yielded only a limited number of studies in which VNs with PCs were included in polymeric dressings for wound healing [ 31 , 94 ]. Table 6 presents VNs bearing HEs or PCs incorporated in topical systems.…”

Section: Topical Delivery Systems Containing Herbal Extractsmentioning

confidence: 99%

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Vesicular Nanocarriers for Phytocompounds in Wound Care: Preparation and Characterization

2022

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The need to develop wound healing preparations is a pressing challenge given the limitations of the current treatment and the rising prevalence of impaired healing wounds. Although herbal extracts have been used for many years to treat skin disorders, due to their wound healing, anti-inflammatory, antimicrobial, and antioxidant effects, their efficacy can be questionable because of their poor bioavailability and stability issues. Nanotechnology offers an opportunity to revolutionize wound healing therapies by including herbal compounds in nanosystems. Particularly, vesicular nanosystems exhibit beneficial properties, such as biocompatibility, targeted and sustained delivery capacity, and increased phytocompounds’ bioavailability and protection, conferring them a great potential for future applications in wound care. This review summarizes the beneficial effects of phytocompounds in wound healing and emphasizes the advantages of their entrapment in vesicular nanosystems. Different types of lipid nanocarriers are presented (liposomes, niosomes, transferosomes, ethosomes, cubosomes, and their derivates’ systems), highlighting their applications as carriers for phytocompounds in wound care, with the presentation of the state-of-art in this field. The methods of preparation, characterization, and evaluation are also described, underlining the properties that ensure good in vitro and in vivo performance. Finally, future directions of topical systems in which vesicle-bearing herbal extracts or phytocompounds can be incorporated are pointed out, as their development is emerging as a promising strategy.

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“…This process can be accelerated by using an ultrasound bath. The resulting large MLVs are subjected to sonication, finally obtaining small unilamellar vesicles (SUVs) [ 94 , 97 ].…”

Section: Preparation Methods Of Vesicular Nanosystemsmentioning

confidence: 99%

Section: Preparation Methods Of Vesicular Nanosystemsmentioning

confidence: 99%

Section: Topical Delivery Systems Containing Herbal Extractsmentioning

confidence: 99%

Section: Topical Delivery Systems Containing Herbal Extractsmentioning

confidence: 99%

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Vesicular Nanocarriers for Phytocompounds in Wound Care: Preparation and Characterization

2022

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“…In addition, Bromelain application resulted in significantly increased fibroblasts by the 5 th day after treatment, which again decreased by the 7 th day after treatment. On the other hand, Miranda et al (2021) [108] developed membranes composed of carboxymethyl cellulose/acetylated arrowroot starch incorporated with bromelain extract-loaded nanoparticles (NPs) and liposomes (LIPs). In the healing potential assessments, no edema was revealed in the histological analysis of the animals treated with these membranes incorporated with bromelain-loaded NPs and LIPs on the 14 th day after treatment.…”

Section: Wound Healing Activity In Vitromentioning

confidence: 99%

Biochemical Characteristics and Healing Activity of Bromelia laciniosa Leaf Protease

Silva¹,

Nerys²,

Silva³

et al. 2022

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Macambira plant (Bromelia laciniosa Mart. Ex Shult. & Schult. f.) is a species belonging to the family Bromeliaceae, which is distributed widely in the hinterlands of northeastern Brazil, from Bahia to Piauí states. The plants of this species contain an enzyme complex named bromelain, which has great potential for therapeutic applications. In the present study, bromelain derived from Bromelia laciniosa leaves was assessed for its thermodynamic properties and in vitro cytotoxic and healing activities. Bromelain was extracted from the leaves and partitioned using ethanol. The partially purified bromelain was then characterized to determine the optimum pH and temperature and the kinetic and thermodynamic parameters. The partially purified bromelain derived from macambira leaves exhibited its highest activity at 47°C and pH 7, when the Km and Vmax values of 2.26 µM and 2.083 U/mL, respectively. The half-life of this bromelain at 47°C was 5.78 h. At 47°C, the Gibbs's free energy, enthalpy, and entropy values reached 89, 53.54, and –0.11 kJ/mol K, respectively. In the SDS-PAGE analysis, bromelain appeared as the main protein band, with an estimated molecular mass of 33 kDa. The in vitro cytotoxic activity and wound healing effects were determined using the MTT assay and Scratch assay, respectively. These assays were performed on the non-neoplastic cell lines L-929 and PBMC and the human tumor cell lines MCF-7, HCT-116, and NCI-H292. Bromelain exhibited a positive healing activity with a change rate similar to that observed for the control. In addition, bromelain exhibited cytotoxic activity against NCI-H292 tumor cells and chemopreventive potential. These results indicate that bromelain derived from macambira leaves may be utilized as a potential ingredient in pharmaceutical formulations, for various healing and chemopreventive purposes and as an alternative to the bromelain extracted from pineapple. In addition, the biochemical characteristics of this bromelain could find application in the food industry. Future studies should, therefore, focus on deciphering the action mechanisms underlying the activities of bromelain observed in the present study to further confirm and enhance its application potential for therapeutic purposes.

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Polysaccharide hydrogel platforms as suitable carriers of liposomes and extracellular vesicles for dermal applications

Pourtalebi Jahromi,

Rothammer,

Fuhrmann

2023

Advanced Drug Delivery Reviews

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Development of membranes based on carboxymethyl cellulose/acetylated arrowroot starch containing bromelain extract carried on nanoparticles and liposomes

Cited by 7 publications

References 34 publications

Vesicular Nanocarriers for Phytocompounds in Wound Care: Preparation and Characterization

Vesicular Nanocarriers for Phytocompounds in Wound Care: Preparation and Characterization

Biochemical Characteristics and Healing Activity of Bromelia laciniosa Leaf Protease

Polysaccharide hydrogel platforms as suitable carriers of liposomes and extracellular vesicles for dermal applications

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