Development of magnetic solid phase extraction platform for the purification of bioactive γ-glutamyl peptides from garlic (Allium sativum)

Yu, Xi; Lim, Cerise Yin Xin; Dong, Bingxue; Hadinoto, Kunn

doi:10.1016/j.lwt.2020.109410

Cited by 18 publications

(15 citation statements)

References 23 publications

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“…The most representative extraction method in immunocapture is magnetic bead extraction, and the main advantage is that magnetic beads provide a large surface area for exosome capture to improve the extraction efficiency. Because magnetic bead extraction can separate exosomes from specific cells, and this method is highly specific, the purity of the product is high and is suitable for marker detection and clinical diagnosis of exosomes ( 68 , 69 ). However, its application is limited to samples with a certain surface target for immune adsorption and the cost is high.…”

Section: Extraction Methods Of Breast Milk Exosomesmentioning

confidence: 99%

Recent Advances on the Function and Purification of Milk Exosomes: A Review

Zhang

et al. 2022

Front. Nutr.

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Exosomes are nano-scale extracellular vesicles, which can be used as drug carriers, tumor treatment, intestinal development and immune regulator. That is why it has great potential in pharmacy, functional foods, nutritional supplements, especially those for infants, postoperative patients, chemotherapy patients and the elderly. In addition, abnormal exosome level is also related to diseases such as cardiovascular diseases, tumor, diabetes, neurodegenerative and autoimmune diseases, as well as infectious diseases. Despite its high biological significance, pharmaceutical and nutritional value, the low abundancy of exosomes in milk is one of the bottlenecks restricting its in-depth research and real-life application. At present, there is no unified standard for the extraction of breast milk exosomes. Therefore, choosing the proper extraction method is very critical for its subsequent research and development. Based on this, this paper reviewed the purification techniques, the function and the possible applications of milk exosomes based on 47 latest references. Humble advices on future directions, prospects on new ideas and methods which are useful for the study of exosomes are proposed at the end of the paper as well.

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Section: Extraction Methods Of Breast Milk Exosomesmentioning

confidence: 99%

Recent Advances on the Function and Purification of Milk Exosomes: A Review

Zhang

et al. 2022

Front. Nutr.

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“…Hydrophobic alkyl chains, molecularly imprinted polymers, and carbon-based material such as graphene oxide are used to modify the MNPs to achieve better analysis efficiency. Noticeably, certain pretreatment steps, such as grinding and ultrasonic cell disruption, are needed prior to the extraction with MNPs to release the organic pollutants from where they are originally . And liquid–solid extraction is used sometimes to dissolve the target analyte and transfer them into a solution so that MSPE can be carried out .…”

Section: Applicationmentioning

confidence: 99%

“…Such characteristics can be applied as a powerful tool for easily controllable capture and release of low abundancy target analytes in bulk samples. In addition, the MNP surface can be modified with polymeric properties, which present broad application prospects in analytical science, food safety monitoring, biomedicine, immunoassay, magnetic resonance imaging, immobilization enzymes, environmental engineering, and proteomics, among many other fields. − Moreover, most MNPs are also environmentally friendly, which often can be recollected and used multiple times to avoid pollution. And a large number of food safety analysis methods, typified by magnetic solid-phase extraction (MSPE), have been developed based on these magic magnetic nanohelpers. , …”

Section: Introductionmentioning

confidence: 99%

Design, Preparation, and Application of Magnetic Nanoparticles for Food Safety Analysis: A Review of Recent Advances

Zhong

Zhang

et al. 2021

J. Agric. Food Chem.

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This review (with 126 references) aims at providing an updated overview of the recent developments and innovations of the preparation and application of magnetic nanoparticles for food safety analysis. During the past two decades, various magnetic nanoparticles with different sizes, shapes, and surface modifications have been designed, synthesized, and characterized with the prospering development of material science. Analytical scientists and food scientists are among the ones who bring these novel materials from laboratories to commercial applications. Powerful and versatile surface functional groups and high surface to mass ratios make these magnetic nanoparticles useful tools for high-efficiency capture and preconcentration of certain molecules, even when they exist in trace levels or complicated food matrices. This is why more and more methods for sensitive detection and quantification of hazards in foods are developed based on these magic magnetic tools. In this review, the principles and superiorities of using magnetic nanoparticles for food pollutant analysis are first introduced, like the mechanism of magnetic solid phase extraction, a most commonly used method for food safety-related sample pretreatment. Their design and preparation are presented afterward, alongside the mechanisms underlying their application for different analytical purposes. After that, recently developed magnetic nanoparticle-based methods for dealing with food pollutants such as organic pollutants, heavy metals, and pathogens in different food matrices are summarized in detail. In the end, some humble outlooks on future directions for work in this field are provided.

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“…In the MSPE technique, a magnetic adsorbent was used to adsorb the target analyte (extraction process). The extraction process can be done by suspending magnetic adsorbents with a sample solution by mixing [4]. After the extraction process, the magnetic adsorbent is separated using an external magnet (a separation process).…”

Section: Introductionmentioning

confidence: 99%

“…The magnetic nanoparticle can be functionalized with organic, inorganic, and metal-organic framework compounds [10][11][12]. At present, there are many developments in magnetic solidphase extraction, including the development of MSPE for the separation of active substances in biological samples [13], purification in extracts [4], pesticides in fruit and vegetables [14]. This review aims to inform about the magnetic nanoparticle functionalization and an application of solid magnetic phase extraction to separate analytes in various types of samples.…”

Section: Introductionmentioning

confidence: 99%

Review: Application of Magnetic Solid-Phase Extraction (Mspe) in Various Types of Samples

Holik

2021

Int J App Pharm

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This review aimed to inform about the magnetic nanoparticle functionalization and solid magnetic phase extraction application to separate analytes in various types of samples. The review was conducted by analyzing several articles published in 2010 until 2021 obtained through search engines, such as Science Direct, Google Scholar and PubMed, using the keyword “magnetic phase extraction” and “magnetic nanoparticle”. The magnetic nanoparticle can be functionalized with organic, inorganic, and metal-organic framework compounds to obtain good selectivity and extraction capability. The Magnetic Solid Phase Extraction (MSPE) can be applied to separate analytes in biological, food, environmental samples. The MSPE can be used in various biological, food, and environmental samples resulting in high enrichment factor value, good recovery, and the magnetic adsorbent has excellent reusability.

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Development of magnetic solid phase extraction platform for the purification of bioactive γ-glutamyl peptides from garlic (Allium sativum)

Cited by 18 publications

References 23 publications

Recent Advances on the Function and Purification of Milk Exosomes: A Review

Recent Advances on the Function and Purification of Milk Exosomes: A Review

Design, Preparation, and Application of Magnetic Nanoparticles for Food Safety Analysis: A Review of Recent Advances

Review: Application of Magnetic Solid-Phase Extraction (Mspe) in Various Types of Samples

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