Development of magnetic nanoparticle based calorimetric assay for the detection of bovine mastitis in cow milk

Chinnappan, Raja; Attas, Sana Al; Kaman, Wendy E.; Bikker, Floris J.; Zourob, Mohammed

doi:10.1016/j.ab.2017.02.009

Cited by 25 publications

(15 citation statements)

References 30 publications

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“…The bovine mammary epithelial cell participates in the innate immune response by producing pro‐ and anti‐inflammatory factors, antimicrobial peptides, and nitric oxide (Rainard and Riollet, ; Schukken et al, ). In mastitis, the concentration and bioactivity of inflammatory cytokines such as ILs and TNF‐α increases (Persson Waller, Colditz, Lun, & Ostensson, ) These inflammatory responses result in deterioration of milk proteins and affect milk protein composition and quantity changes in negative way (Chinnappan, Al Attas, Kaman, Bikker, & Zourob, ). A previous study reported that LPS induced innate inflammation in bovine mammary gland cells and increased the levels of IL‐1β , IL‐8 , and TNF‐α mRNAs (Strandberg et al, ).…”

Section: Discussionmentioning

confidence: 99%

Bifunctional role of ephrin A1‐Eph system in stimulating cell proliferation and protecting cells from cell death through the attenuation of ER stress and inflammatory responses in bovine mammary epithelial cells

Kang

Jeong

Bae

et al. 2017

Journal Cellular Physiology

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Structural and functional development of the mammary gland is constant in the mammary gland life cycle. Eph receptors and their ligands, ephrins, control events through cell-to-cell interactions during embryonic development, and adult tissue homeostasis; however, little information on participation of ephrin A1, a representative ligand of the Eph receptor, in the development and function of normal mammary glands is known. In this study, we demonstrated functional effects of the ephrin A1-Eph system and mechanisms of its action on bovine mammary epithelial (MAC-T) cells. The in vitro cultured MAC-T cells expressed the ephrin A1 ligand and EphA1, A2, A4, A7, and A8 among the eight members of the Eph A family. Our results revealed that ephrin A1 induced MAC-T cell cycle progression and stimulated cell proliferation with abundant expression of nucleic PCNA and cyclin D1 proteins. Additionally, ephrin A1 induced activation of intracellular signaling molecules involved in PI3 K/AKT and MAPK signaling, and the proliferation-stimulating effect of ephrin A1 was mediated by activation of these pathways. Furthermore, ephrin A1 influenced expression and activation of various ER stress-related proteins and protected MAC-T cells from stress-induced cell death. Finally, ephrin A1 alleviated LPS-induced cell death through down-regulation of inflammatory cytokines. In conclusion, the results of this study suggest that the Eph A-ephrin A1 system is a positive factor in the increase and maintenance of epithelial cells in mammary glands of cows; the signaling system contributes to development, remodeling, and functionality of normal mammary glands and could overcome mastitis in cows and other mammals.

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Section: Discussionmentioning

confidence: 99%

Bifunctional role of ephrin A1‐Eph system in stimulating cell proliferation and protecting cells from cell death through the attenuation of ER stress and inflammatory responses in bovine mammary epithelial cells

Kang

Jeong

Bae

et al. 2017

Journal Cellular Physiology

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“…Recently, several other paper colorimetric biosensors based on the proteolytic activity of the target have also been reported to detect food contaminants in complex foods and milk. 253,254 However, in recent decades, many MNP-colorimetric sensors have been reported based on the intrinsic peroxidase mimetic activity of MNPs, which is described separately in section 5.3.…”

Section: Colorimetry and Fluorescence Detectionmentioning

confidence: 99%

Superparamagnetic nanoarchitectures for disease-specific biomarker detection

et al. 2019

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“…The conjugation of magnetic beads with the aptamers was confirmed by a shift in the IR vibration frequency of the carbonyl group from 1725 cm −1 to 1650 cm −1 . After conjugation, the carboxylic acids were converted to amide and the IR absorption at 1725 cm −1 and 1650 cm −1 corresponded to the carbonyl group in the carboxylic acid and amide, respectively [ 26 ] ( Figure S1 ).…”

Section: Resultsmentioning

confidence: 99%

Anti-VCAM-1 and Anti-IL4Rα Aptamer-Conjugated Super Paramagnetic Iron Oxide Nanoparticles for Enhanced Breast Cancer Diagnosis and Therapy

et al. 2020

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The surface protein overexpressed on cancer cells can be used as biomarkers for early detection of specific diseases. Anti-VCAM-1 and anti-IL4Rα DNA aptamers specific to VCAM-1 and IL4Rα receptors that are overexpressed in 4T1 tumor-bearing mice could be used as potential biomarker for both diagnostic and therapeutic applications in cancer biology. Cell Viability and luciferase assay of 4T1-Luc2 cancer cells in the presence of anti-VCAM-1 ssDNA or anti-IL4Rα RNA aptamers was assessed by monitoring the changes in the absorbance and the fluorescence of Alamar blue dye. The aptamer-conjugated SPIO magnetic beads, used for the selective targeting to tumor sites, were monitored using noninvasive MRI and Bioluminescence imaging (BLI). Cell viability and luciferase assays showed that both anti-VCAM-1 and anti-IL4Rα aptamers favor the depletion of cancer cells and limit tumor progression. Microscopic analyses confirmed that the target specific aptamers significantly trigger tumor cell apoptosis and limit cancer cell growth in vitro. The intravenous injection of SPIO nanoparticle-conjugated aptamers were further confirmed using noninvasive MRI and Bioluminescence imaging. Anti-VCAM1 and anti-IL4Rα aptamers, specific to VCAM-1 and IL4Rα receptors overexpressed in 4T1-Luc2 tumor-bearing mice, were used as diagnostic and therapeutic tools.

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Development of magnetic nanoparticle based calorimetric assay for the detection of bovine mastitis in cow milk

Cited by 25 publications

References 30 publications

Bifunctional role of ephrin A1‐Eph system in stimulating cell proliferation and protecting cells from cell death through the attenuation of ER stress and inflammatory responses in bovine mammary epithelial cells

Bifunctional role of ephrin A1‐Eph system in stimulating cell proliferation and protecting cells from cell death through the attenuation of ER stress and inflammatory responses in bovine mammary epithelial cells

Superparamagnetic nanoarchitectures for disease-specific biomarker detection

Anti-VCAM-1 and Anti-IL4Rα Aptamer-Conjugated Super Paramagnetic Iron Oxide Nanoparticles for Enhanced Breast Cancer Diagnosis and Therapy

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