Development of light-evoked changes of the interphotoreceptor matrix in normal and RCS rats with inherited retinal dystrophy

Uehara, F; Yasumura, Douglas; LaVail, Matthew M.

doi:10.1016/0014-4835(91)90144-4

Cited by 14 publications

(13 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This characteristic pattern of staining conforms to the previous studies in which white-light-adapted rat retinas showed a similar banded binding with colloidal iron and WGA [5][6][7], By contrast, a uniform stain ing of the IPM throughout the interstitial zone of the OS was found when the animals were exposed to red light. This observeration is compatible with that in dark-adapted rather than light-adapted retinas in the previous studies [5][6][7], Two types of morphologically and func tionally distinct photoreceptors exist in the rat retina, i.e., typical rods and photopicfunctioning cone-like photoreceptors [10,11]; this has also been evident in histochemical studies using a lectin, peanut agglutinin, which binds preferentially to the IPM associ ated with the cones or cone-like cells [6,[12][13][14]. The intensity of the red light employed in the present experiment was comparable to the green light when evaluated in terms of the photopic vision, while it was much less effective for the scotopic or rod vision.…”

Section: Discussionsupporting

confidence: 67%

“…Recent studies of the IPM using histochemical probes including colloidal iron, lectins and antibodies against interphotoreceptor retinoid binding protein have demonstrated that its distribution is changeable with the lighting condition before preparation of tissues; in the dark, the IPM surrounding the interstitial zone of photore ceptor outer segments (OS) is uniformly bound by the probes, whereas in the light, the apical and basal zones of OS and the apical zone of the inner segment (IS) show a distinct staining [5,6]. The changeable distribution of the IPM with the lighting condition is likely to represent not a circadian rhythm but local retinal effects [5], and the use of lectins spe cific for cones or rods suggests a predominant contribution of rod photoreceptors to the light-evoked changes of 1PM [7], Previous studies used white light to evoke the light pat tern of IPM distribution [5][6][7], This study used various colored lights to determine whether the light-evoked changes of IPM are associated with the wavelength of light illuminated.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Interphotoreceptor Matrix in the Colored-Light-Adapted Rat

Nakashima

Uehara²,

Ohba³

1992

Ophthalmic Res

Self Cite

View full text Add to dashboard Cite

The interphotoreceptor matrix (IPM) was examined histochemically using colloidal iron, wheat germ agglutinin and Ricinus communis agglutinin-1 for rats adapted to blue, green or red light before tissue preparation. In blue- and green-light-adapted animals, IPM was intensely stained in the apical zone of photoreceptor outer segments and in the outer and inner segment junction, conforming to the light pattern of IPM distribution in previous studies. In red-light-adapted animals, the IPM was prominent and uniform in the interstitial zone of photoreceptor outer segments, consistent with the dark pattern. The results indicate that rod photoreceptors are predominantly responsible for light-evoked changes in IPM.

show abstract

Section: Discussionsupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Interphotoreceptor Matrix in the Colored-Light-Adapted Rat

Nakashima

Uehara²,

Ohba³

1992

Ophthalmic Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…An accompanying increase in fluorescein leakage of RPE was observed [Sollom and Adlakha, 1968] indicating the breakdown of the epithelial barrier of the RPE. An accumulation of interphotoreceptor matrix proteins at the basal outer segment zone occurs [Uehara et al, 1991]. As a consequence, apoptosis of photoreceptor cells is initiated [Tso et al, 1994] and microglial cells are attracted from the ganglion cell layer [Thanos, 1992;Roque et al, 1996].…”

Section: Retinal Dystrophy In Rcs Ratsmentioning

confidence: 99%

The Royal College of Surgeons Rat: An Animal Model for Inherited Retinal Degeneration with a Still Unknown Genetic Defect

Strauß

Stumpff²,

Mergler³

et al. 1998

Cells Tissues Organs

View full text Add to dashboard Cite

The Royal College of Surgeons (RCS) rat is the first known animal with inherited retinal degeneration. Despite the fact that the genetic defect is not known, the RCS rat is widely used for research in hereditary retinal dystrophies. This review tries to summarize observations which have been made in the RCS rat and to make an attempt to formulate candidate genes which may the cause for the retinal degeneration in this rat strain. The genetic defect in RCS rats causes the inability of the retinal pigment epithelium (RPE) to phagocytose shed photoreceptor outer segments. In normal rats or humans, this circadian process is regulated by both the cyclic adenosine monophosphate (cAMP) and the calcium/ inositol phosphate systems. The calcium/inositol phosphate system seems to be linked to the phagocytosis receptors which recognize photoreceptor outer membranes to initialize phagocytosis. The cAMP system appeared as modulator of the regulation of phagocytosis. An increase in the intracellular cAMP concentration is an ‘off’ signal for phagocytosis. In RPE cells from RCS rats many observations have been made which indicate a changed second messenger metabolism concerning both the cAMP and the calcium/inositol phosphate systems. The genetic defect seems to concern a protein which is involved in the initialization of a second messenger pathway. We conclude that the genes coding for the phagocytosis receptor or for proteins which are linked to receptors (for example G proteins) are good candidates for defective genes in RCS rats.

show abstract

“…PNA and anti-C6S an tibody binding is conspicuously absent from corresponding regions of 1PM associated with rods [6][7][8]. Relatively little is known about the function of the cone matrix sheath, although the matrix potentially mediates adhesion be tween the photoreceptors and the pigment epithelium [9][10][11], Recent immunohistochemical analyses have confirmed that dynamic changes in mo lecular distribution arc evoked in the 1PM by dark/light adaptation [12,13]. In addition, Yamamoto et al [14] and Yamamoto and Steinberg [15][16][17] described light/dark changes in pH near the IPM using H+-sclective microelectrodes.…”

Section: Introductionmentioning

confidence: 99%

pH-Dependent Changes in interphotoreceptor Matrix Domains Surrounding Cone Photoreceptors

et al. 1996

View full text Add to dashboard Cite

The cone matrix sheath is a biochemcially and structurally distinct domain of the retinal interphotoreceptor matrix (IPM). In the present study, the insoluble components of the cone matrix sheath were extracted from bovine retina, and examined for pH-dependent morphological changes by light and electron microscopy. After soaking unfixed retinas in 25 mM HEPES-buffered solution (pH 2.0), sheets of aqueous-insoluble IPM containing cone matrix sheaths were physically dissociated from the inner retina. The insoluble IPM preparations were treated with 1.0% Triton X-100 to extract contaminating photoreceptor outer segment membrane, and exposed to HEPES-buffered solutions at pH 2.0,4.5,7.0, or 9.5. The cone matrix sheath, specifically stained with peanut agglutinin, appeared finely granular at pH 2.0, but was markedly condensed at pH 4.5. Cone sheath morphology became dispersed and appeared as a network of thick fibrils at pH 7.0, and further dispersed fibrillar and granular elements at pH 9.5. These findings suggest that pH changes in the interphotoreceptor space can alter the molecular organization of the IPM and influence the function of the IPM in the transport of nutrients and metabolites, and in retinal adhesion.

show abstract

Development of light-evoked changes of the interphotoreceptor matrix in normal and RCS rats with inherited retinal dystrophy

Cited by 14 publications

References 21 publications

Interphotoreceptor Matrix in the Colored-Light-Adapted Rat

Interphotoreceptor Matrix in the Colored-Light-Adapted Rat

The Royal College of Surgeons Rat: An Animal Model for Inherited Retinal Degeneration with a Still Unknown Genetic Defect

pH-Dependent Changes in interphotoreceptor Matrix Domains Surrounding Cone Photoreceptors

Contact Info

Product

Resources

About