Development of label-free optical diagnosis for sensitive detection of influenza virus with genetically engineered fusion protein

Park, Tae Jung; Lee, Seok Jae; Kim, Do-Kyun; Heo, Nam Su; Park, Jung Youn; Lee, Sang Yup

doi:10.1016/j.talanta.2011.12.021

Cited by 64 publications

(39 citation statements)

References 31 publications

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“…A metal binding polypeptide (GBP)-fusion protein was bound onto the gold substrates by means of specific interactions allowing immobilization of proteins in bioactive forms onto the gold surface suitable for immunosensors applications. Following this procedure, an ultrasensitive detection of avian influenza virus was successfully achieved up to 1 pg mL −1 [51]. …”

Section: Nanomodified Spr-abbs Applications For In Vitro Diagnosticsmentioning

confidence: 99%

Nanotechnology-Based Surface Plasmon Resonance Affinity Biosensors forIn VitroDiagnostics

Antiochia

Bollella

Favero

et al. 2016

International Journal of Analytical Chemistry

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In the last decades, in vitro diagnostic devices (IVDDs) became a very important tool in medicine for an early and correct diagnosis, a proper screening of targeted population, and also assessing the efficiency of a specific therapy. In this review, the most recent developments regarding different configurations of surface plasmon resonance affinity biosensors modified by using several nanostructured materials for in vitro diagnostics are critically discussed. Both assembly and performances of the IVDDs tested in biological samples are reported and compared.

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Section: Nanomodified Spr-abbs Applications For In Vitro Diagnosticsmentioning

confidence: 99%

Nanotechnology-Based Surface Plasmon Resonance Affinity Biosensors forIn VitroDiagnostics

Antiochia

Bollella

Favero

et al. 2016

International Journal of Analytical Chemistry

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“…These glycoNPs were able to distinguish between human (a-2,6 binding) and avian (a-2,3 binding) RG14 (H5N1) influenza viruses [78]. The GBP-fusion method allows the proteins to be immobilized in their bioactive forms, facilitating the study of antigen-antibody interactions [80]. AuNPs additionally provide an intense signal that is visible with the naked eye [24].…”

Section: Aunp-based Aiv Biosensorsmentioning

confidence: 99%

Advanced nanotechnologies in avian influenza: Current status and future trends – A review

Moulick

Richtera

Milosavljević

et al. 2017

Analytica Chimica Acta

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In the last decade, the control of avian influenza virus has experienced many difficulties, which have caused major global agricultural problems that have also led to public health consequences. Conventional biochemical methods are not sufficient to detect and control agricultural pathogens in the field due to the growing demand for food and subsidiary products; thus, studies aiming to develop potent alternatives to conventional biochemical methods are urgently needed. In this review, emerging detection systems, their applicability to diagnostics, and their therapeutic possibilities in view of nanotechnology are discussed. Nanotechnology-based sensors are used for rapid, sensitive and cost-effective diagnostics of agricultural pathogens. The application of different nanomaterials promotes interactions between these materials and the virus, which enables researchers to construct portable electroanalytical biosensing analyser that should effectively detect the influenza virus. The present review will provide insights into the guidelines for future experiments to develop better techniques to detect and control influenza viruses.

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“…In BLI assay II, a genetically engineered GBP-Fab anti-CFP-10 Ab was employed as a target Ag recognition molecule, which was bound to the tip surface via an Au NP-assisted method. The GBP region has a specific and strong affinity for the Au (1 1 1) planes [23,24], which is highly advantageous for not only stable and reliable analysis but also facile protein conjugation [25][26][27]. To take advantage of the GBP-fusion Abs, 25-nm Au NPs were attached to the APS biosensor tip in advance.…”

Section: Evaluation Of the 3 Ab Immobilization Strategiesmentioning

confidence: 99%

Clinical immunosensing of tuberculosis CFP-10 antigen in urine using interferometric optical fiber array

Kim¹,

Hong²,

Hong³

et al. 2015

Sensors and Actuators B: Chemical

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Development of label-free optical diagnosis for sensitive detection of influenza virus with genetically engineered fusion protein

Cited by 64 publications

References 31 publications

Nanotechnology-Based Surface Plasmon Resonance Affinity Biosensors forIn VitroDiagnostics

Nanotechnology-Based Surface Plasmon Resonance Affinity Biosensors forIn VitroDiagnostics

Advanced nanotechnologies in avian influenza: Current status and future trends – A review

Clinical immunosensing of tuberculosis CFP-10 antigen in urine using interferometric optical fiber array

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